In vitro inhibitory activity of human vaginal lactobacilli against pathogenic bacteria associated with bacterial vaginosis in Kenyan women |
| |
| Authors: | Martin N Matu George O Orinda Eliud NM Njagi Craig R Cohen Elizabeth A Bukusi |
| |
| Institution: | 1. Department of Biochemistry and Biotechnology, Kenyatta University, P.O. Box 43844, 00100, Nairobi, Kenya;2. Kenya Medical Research Institute, Centre for Microbiology Research, P.O. Box 19464, 00202 Nairobi, Kenya;3. Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, USA;1. Shamakhy Astrophysical Observatory named after N.Tusi, ANAS, Baku, Azerbaijan;2. Qafqaz University, Qafqaz University Campus, Khirdalan c/Baku, Azerbaijan;1. Magee-Womens Research Institute, 204 Craft Avenue, Pittsburgh, PA 15213, USA;2. University of Pittsburgh Department of Obstetrics, Gynecology and Reproductive Sciences, 300 Halket Street, Pittsburgh, PA 15213, USA;1. Winclove Probiotics, 11 Hulstweg, 1032 LB Amsterdam, The Netherlands;2. KU Leuven, Centre of Microbial and Plant Genetics, Kasteelpark Arenberg 20, 3001 Leuven, Belgium;3. University of Antwerp, Department of Bioscience Engineering, Antwerp, Belgium;4. McMaster University, Department of Family Medicine, 100 Main Street West Hamilton, ON L8P 1H6, Canada;5. University Medical Center Groningen, Department of Biomedical Engineering, Antonius Deusinglaan 1, 9713AV Groningen, The Netherlands;6. Human Microbiology and Probiotics, Lawson Health Research Institute, 268 Grosvenor Street, London, Ontario, N6A 4V2, Canada;7. Departments of Microbiology & Immunology, and Surgery, The University of Western Ontario, London, Ontario, N6A 5C1, Canada;1. MEDIOGEN, Co., Ltd, Seoul, 04157, Republic of Korea;2. Department of Biological Engineering, Inha University, Incheon 22212, Republic of Korea;1. HIV/STD Unit, Institute of Tropical Medicine, Antwerp, Belgium;2. HIV/STI Reference Laboratory, Institute of Tropical Medicine, Antwerp, Belgium;3. Division of Infectious Diseases and HIV Medicine, University of Cape Town, Cape Town, South Africa |
| |
| Abstract: | Lactobacilli have been shown to inhibit in vitro growth of many pathogens and have been used as probiotics to treat a broad range of gastrointestinal and/or vaginal disorders. We sought to determine the in vitro inhibitory potential of lactobacilli of vaginal origin to some bacteria associated with bacterial vaginosis (BV), to characterize the inhibitory substances produced by these lactobacilli and to assess H2O2 production. Vaginal specimens were obtained by swabbing the lateral vaginal walls from 107 women two months following BV treatment. One hundred and fifty eight Lactobacillus spp. were isolated in 82 of the 107 women. Lactobacillus jensenii was the predominant strain isolated among these women (29/158; 18.4%). Among 158 culture supernatants tested for antibacterial activity against BV-associated bacteria, none inhibited the growth of Bacteroides fragilis while 23% (37/158), 28% (45/158) and 29% (46/158) inhibited the growth of Prevotella bivia, Gardnerella vaginalis and Mobiluncus spp. respectively. The lactobacilli produced supernatants with a pH range between 2.62 and 6.71; the highly acidic (pH 2–3.99) supernatants were more inhibitory to the indicator strains. There was significant reduction in the mean zones of inhibition following chemical and physical treatment of the supernatants (p = 0.0025). Acid, bacteriocins and H2O2 demonstrated potential for antagonism of the bacterial pathogens. These substances may augment each other rather that each working independently on the pathogens. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
