Molecular phylogenetic diversity of the emerging mucoralean fungus Apophysomyces: Proposal of three new species |
| |
Authors: | Eduardo Alvarez Alberto M Stchigel Josep Cano Deanna A Sutton Annette W Fothergill Jagdish Chander Valentina Salas Michael G Rinaldi Josep Guarro |
| |
Institution: | 1. Mycology Unit, Medical School and IISPV, Universitat Rovira i Virgili, Reus, Spain;2. Fungus Testing Laboratory, University of Texas Health Science Center, San Antonio, TX, USA;3. Government Medical College and Hospital, Chandigarh, India |
| |
Abstract: | BackgroundApophysomyces is a monotypic genus belonging to the order Mucorales. The species Apophysomyces elegans has been reported to cause severe infections in immunocompromised and immunocompetent people. In a previous study of Álvarez et al.3 J Clin Microbiol 2009;47:1650–6], we demonstrated a high variability among the 5.8S rRNA gene sequences of clinical strains of A. elegans.Material and methodsWe performed a polyphasic study based on the analysis of the sequences of the histone 3 gene, the internal transcribed spacer region of the rDNA gene, and domains D1 and D2 of the 28S rRNA gene, as well as by evaluation of some relevant morphological and physiological characteristics of a set of clinical and environmental strains of A. elegans.Results and conclusionsWe have demonstrated that A. elegans is a complex of species. We propose as new species Apophysomyces ossiformis, characterised by bone-shaped sporangiospores, Apophysomyces trapeziformis, with trapezoid-shaped sporangiospores, and Apophysomyces variabilis, with variable-shaped sporangiospores. These species failed to assimilate esculin, whereas A. elegans was able to assimilate that glycoside. Amphotericin B and posaconazole are the most active in vitro drugs against Apophysomyces. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|