Apolipophorin-III expression and low density lipophorin formation during embryonic development of the silkworm,Bombyx mori |
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| Authors: | Kozo Tsuchida Takeru Yokoyama Takashi Sakudoh Chihiro Katagiri Shuichiro Tsurumaru Naoko Takada Hirofumi Fujimoto Rolf Ziegler Hidetoshi Iwano Kunikatsu Hamano Toshinobu Yaginuma |
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| Affiliation: | 1. Division of Radiological Protection and Biology, National Institute of Infectious Diseases, Tokyo, Japan;2. Institute of Low Temperature Science, Hokkaido University, Sapporo, Japan;3. Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan;4. Department of Entomology, University of Arizona, Tucson, AZ85721, USA;5. College of Bioresource Sciences, Nihon University, Fujisawa, Japan;6. Department of International Environmental and Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Japan |
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| Abstract: | We examined the expression of apolipophorin-III (apoLp-III) during embryonic development of the silkworm Bombyx mori. ApoLp-III mRNA was first expressed 24 h after oviposition, which corresponds to the time of germ band formation. The amount of apoLp-III in the eggs increased from day 2, peaked on day 4, and then gradually decreased until hatching (on day 9.5). ApoLp-III was apparently synthesized during early embryogenesis, as radioactive amino acids were incorporated into newly synthesized apoLp-III in three-day-old eggs. Moreover, radioactive apoLp-III was found only in the embryo and not in the extraembryonic tissue. KBr density gradient ultracentrifugation of egg homogenates showed that apoLp-III was associated with low-density lipophorin (LDLp). These results suggest that LDLp is required for the delivery of lipids for organogenesis during embryogenesis. |
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