Aspergillus nidulans UDP-glucose-4-epimerase UgeA has multiple roles in wall architecture,hyphal morphogenesis,and asexual development |
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| Authors: | Amira M El-Ganiny Inder Sheoran David AR Sanders Susan GW Kaminskyj |
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| Institution: | 1. Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon SK, Canada S7N 5E2;2. Department of Chemistry, University of Saskatchewan, 110 Science Place, Saskatoon SK, Canada S7N 5C9;1. Department of Life Science, Chonbuk National University, Chonju 561-756, Republic of Korea;2. Department of Molecular Biology, Chonbuk National University, Chonju 561-756, Republic of Korea;3. Division of Life Sciences, Wonkwang University, Iksan 570-749, Republic of Korea;3. Department of Molecular and Cell Biology, University of California, Berkeley, California 94720;4. Department of Plant and Microbial Biology, University of California, Berkeley, California 94720;5. Department of Chemistry, University of California, Berkeley, California 94720;1. Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China;2. Department of Dermatology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210023, China;1. Division of Life Science, Daejeon University, Daejeon, 300-716, Republic of Korea;2. Biomedical Omics Team, Korea Basic Science Institute (KBSI), Ohcang, 368-883, Republic of Korea;3. Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, 305-764, Republic of Korea;4. Department of Bio-Analytical Science, University of Science and Technology, Daejeon, 305-333, Republic of Korea;5. Departments of Bacteriology and Genetics, The University of Wisconsin–Madison, Madison, WI, 53706, USA |
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| Abstract: | Aspergillus nidulans UDP-glucose-4-epimerase UgeA interconverts UDP-glucose and UDP-galactose and participates in galactose metabolism. The sugar moiety of UDP-galactose is predominantly found as galactopyranose (Galp, the six-membered ring form), which is the substrate for UDP-galactopyranose mutase (encoded by ugmA) to generate UDP-galactofuranose (Galf, the five-membered ring form) that is found in fungal walls. In A. fumigatus, Galf residues appear to be important for virulence. The A. nidulans ugeAΔ strain is viable, and has defects including wide, slow growing, highly branched hyphae and reduced conidiation that resemble the ugmAΔ strain. As for the ugmAΔ strain, ugeAΔ colonies had substantially reduced sporulation but normal spore viability. Conidia of the ugeAΔ strain could not form colonies on galactose as a sole carbon source, however they produced short, multinucleate germlings suggesting they ceased to grow from starvation. UgeA purified from an expression plasmid had a relative molecular weight of 40.6 kDa, and showed in vitro UDP-glucose-4-epimerase activity. Transmission electron microscope cross-sections of wildtype, ugeAΔ, and ugmAΔ hyphae showed they had similar cytoplasmic contents but the walls of each strain were different in appearance and thickness. Both deletion strains showed increased substrate adhesion. Localization of UgeA-GFP and UgmA-GFP was cytoplasmic, and was similar on glucose and galactose. Neither gene product had a longitudinal polarized distribution. Localization of a UgmA-mRFP in a strain that resembled the ugmAΔ strain was cytoplasmic and lacked a longitudinal polarized distribution. The roles of UgeA in A. nidulans growth and morphogenesis are consistent with the importance of Galf, and are related but not identical to the roles of UgmA. |
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