Screening and selection of stress resistant Lactobacillus spp. isolated from the marine oyster (Crassostrea gigas) |
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| Authors: | Hae-In Lee Min Hee Kim Kwan Young Kim Jae-Seong So |
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| Affiliation: | 1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China;3. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, 266000 Qingdao, China;1. Departamento de Microbiología y Parasitología, CIBUS-Facultad de Biología, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain;2. Instituto Tecnolóxico para o Control do Medio Mariño de Galicia (INTECMAR), 36611, Vilagarcía de Arousa, Spain;3. Consello Regulador da Denominación de Orixe Protexida do Mexillón de Galicia, 36600 Vilagarcía de Arousa, Spain;1. University of Bremen, Bremen Marine Ecology (BreMarE), P.O. Box 330440, 28359 Bremen, Germany;2. Alfred Wegener Institute for Polar and Marine Research (AWI), Am Handelshafen 12, 27550 Bremerhaven, Germany;3. Bremerhaven University of Applied Sciences, Applied Marine Biology, An der Karlstadt 8, 27568 Bremerhaven, Germany;1. Aquaculture Research Group, Laboratory of Feeds and Feeding Management, School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia;2. Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, Kuala Terengganu, Terengganu, Malaysia;3. Faculty of Fisheries and Aqua-Industry, Universiti Malaysia Terengganu, Kuala Terengganu, Terengganu, Malaysia;4. Department of Fisheries, Government of Sindh, Pakistan |
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| Abstract: | We attempted to isolate Lactobacillus spp. from the marine oyster (Crassostrea gigas) and select stress resistant strains for development of a future marine aquaculture feed adjuvant. A total of 83 lactobacilli strains were isolated from oyster. They were all Gram-positive, rod-shaped and catalase-negative. By performing a stress resistance assay, we selected eighteen isolates. Based on 16S rRNA gene sequencing, Lactobacillus paracasei was the most prevalent species among the selected isolates. The in vitro antagonistic effect of the selected strains against fish pathogens was assayed by measurement of inhibition diameters. Except for MH44, MH51, MH53 and MH62, most of the isolates showed inhibition of Vibrio alginolyticus and Vibrio proteolyticus (diameters over 15 mm). Lactobacillus rhamnosus MH22 was selected as the most stress resistant strain showing the MICs of 1.8 M NaCl, 14% ethanol and 0.014% hydrogen peroxide. L. rhamnosus MH22 isolated from oyster has a potential to be applied as a microbial feed adjuvant for marine aquaculture. |
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