Understanding plasmid effect on hyaluronic acid molecular weight produced by Streptococcus equi subsp. zooepidemicus |
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| Authors: | Esteban Marcellin Wendy Y. Chen Lars K. Nielsen |
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| Affiliation: | 1. Laboratoire Réaction et Génie des Procédés, C.N.R.S.-U.M.R.-7274, plateforme SVS, 13 rue du bois de la Champelle, F-54500 Vand?uvre-lès-Nancy, France;2. Université de Lorraine, U.M.R.-7274, 2 avenue de la forêt de Haye, F-54505 Vand?uvre-lès-Nancy, France;3. Teoxane Geneva, 105 rue de Lyon Les Charmilles, CH1203 Geneva, Switzerland;1. MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China;2. National Research Center of Veterinary Biologicals engineering and Technology, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, China;3. Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, China;4. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China;1. National Institute of Laser Enhanced Sciences, Cairo University, 12613 Giza, Egypt;2. Department of Biochemistry, Faculty of Agriculture, Cairo University, 12613 Giza, Egypt;3. Department of Agricultural Engineering, Faculty of Agriculture, Cairo University, 12613 Giza, Egypt;4. Faculty of Science, Department of Chemistry, Taif University, Taif 21974, Saudi Arabia |
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| Abstract: | Hyaluronic acid is a biopolymer with valuable applications in the pharmaceutical and cosmetic industries. Streptococcus equi subspecies zooepidemicus cells transformed with a nisin-inducible, empty plasmid control displayed higher molecular weight. This increase in molecular weight is independent of the nisin promoter or antibiotic resistance. Using 2D DIGE followed by mass spectrometry, we identified up-regulation of the last step in UDP-N-acetyl-glucosamine biosynthesis (GlmU) and down-regulation of the first step in peptidoglycan biosynthesis (MurA) as possible mechanism for the plasmid effect. Over-expression of GlmU to further increase activity had no effect on UDP-N-acetyl-glucosamine levels or molecular weight, while over-expression of MurA reduced UDP-N-acetyl-glucosamine levels and molecular weight. Global transcriptional analysis revealed that differential regulation of GlmU and MurA activity was not reflected in transcription levels. This results, suggest that regulation is at a translational or post-translational level. Differential expression of two clp proteases may explain this effect as well as the small but significant changes in transcription levels of nearly 300 genes. |
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