An E. coli 5S rRNA Deletion Mutant Useful for the Study of 5S rRNA Structure/Function Relationships |
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Authors: | David Ammons Joanne Rampersad |
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Institution: | (1) School of Veterinary Medicine, University of the West Indies, Mt. Hope, Trinidad, West Indies,;(2) Trinidad and Tobago Aquaproducts, 8A College Trace, St. Augustine, Trinidad, West Indies, |
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Abstract: | We report on the construction of a novel strain of E. coli that can be useful for studies on the structure/function relationship of 5S rRNAs. The bacterial strain is deficient in six
of the eight naturally occurring 5S rRNA genes (operons B, D, H, G, E) and demonstrates a greatly reduced growth rate that
can be compensated by the plasmid-encoded expression of 5S rRNA. The relatively large difference in growth rate between compensated
and non-compensated mutants provides the basis for a quick and simple assaying system for both the evaluation and mass screening
of divergent 5S rRNA sequences for function. We describe the construction of the 5S rRNA deletion mutant BDHGE and characterize
the usefulness and limitations of the system for evaluating structure/function relationships of 5S rRNA sequence.
Received: 20 August 2000 / Accepted: 2 January 2001 |
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