Activation of High-Affinity Uptake of Glutamate by Phorbol Esters in Primary Glial Cell Cultures |
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Authors: | Mariano Casado Francisco Zafra Carmen Aragón Cecilio Giménez |
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Institution: | Centro de Biología Molecular, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain. |
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Abstract: | The effects of 12-O-tetradecanoylphorbol 13-acetate (TPA), a potent activator of protein kinase C, on high-affinity Na(+)-dependent glutamate transport were investigated in primary cultures of neurons and glial cells from rat brain cortex. Incubation of glial cells with TPA led to concentration- and time-dependent increases in the glutamate transport that could be completely suppressed by the addition of the protein kinase C (PKC) inhibitor 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine. The TPA effects could be mimicked by oleoylacetylglycerol and by the diacylglycerol kinase inhibitor R59022. The effects of TPA were potentiated by the Ca2+ ionophore A23187. Under the chosen experimental conditions TPA had no effect on glutamate transport in neurons. We conclude that PKC activates the sodium-dependent high-affinity glutamate transport in glial cells and that it has dissimilar effects on neurons and glial cells. |
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Keywords: | Glutamate uptake Phorbol esters Glial cells Casado |
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