Light-independent accumulation of essential chlorophyll biosynthesis- and photosynthesis-related proteins in <Emphasis Type="Italic">Pinus mugo</Emphasis> and <Emphasis Type="Italic">Pinus sylvestris</Emphasis> seedlings |
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Authors: | K Breznenová V Demko A Pavlovi? E Gálová R Balá?ová J Hudák |
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Institution: | 1.Department of Plant Physiology, Faculty of Natural Sciences,Comenius University,Bratislava,Slovak Republic;2.Department of Genetics, Faculty of Natural Sciences,Comenius University,Bratislava,Slovak Republic |
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Abstract: | Dark-grown seedlings of Pinus mugo Turra and Pinus sylvestris L. accumulate chlorophyll (Chl) and its precursor protochlorophyllide (Pchlide). Pchlide reduction is a key regulatory step
in Chl biosynthesis. In the dark, Pchlide is reduced by light-independent Pchlide oxidoreductase (DPOR) encoded by three plastid
genes chlL, chlN, and chlB (chlLNB). To investigate the differences in chlLNB gene expressions, we compared the dark-grown and 24-h illuminated seedlings of P. mugo and P. sylvestris. Expression of these genes was found constitutive in all analyzed samples. We report light-independent accumulation of important
proteins involved in Chl biosynthesis (glutamyl-tRNA reductase) and photosystem formation (D1 and LHCI). Chl and Pchlide content
and plastid ultrastructure studies were also performed. |
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