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| 截短的猪繁殖与呼吸综合征ORF5基因及重组蛋白的原核表达 | |
| 引用本文： | 李国娟,柳纪省,李宝玉,娄忠子,田永强.截短的猪繁殖与呼吸综合征ORF5基因及重组蛋白的原核表达[J].生物技术通报,2010(3). |
| 作者姓名： | 李国娟 柳纪省 李宝玉 娄忠子 田永强 |
| 作者单位： | 1. 中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室农业部草食动物疫病重点开放实验室,兰州,730046;兰州交通大学化工学院,兰州,730070 2. 中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室农业部草食动物疫病重点开放实验室,兰州,730046 3. 兰州交通大学化工学院,兰州,730070 |
| 摘 要： | 通过PCR方法从重组质粒pGEM-ORF5扩增得到缺失N端疏水序列的基因片段dORF5(deleting ORF5)。将dORF5克隆至原核高效表达载体pGEX-4T-1,在E.coliRosetta细胞中成功表达了重组蛋白GST-dORF5。用Western blotting鉴定表达蛋白,证明dORF5基因得到表达。本试验得到的重组蛋白,为进一步研究PRRS病毒结构蛋白的结构和功能奠定了基础。 |
| 关 键 词： | 猪繁殖与呼吸综合征 ORF5基因 原核表达 |
Prokaryotic Expression of Truncated ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus JXA1 |
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| Li Guojuan,Liu Jixing,Li Baoyu,Lou Zhongzi,Tian Yongqiang.Prokaryotic Expression of Truncated ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus JXA1[J].Biotechnology Bulletin,2010(3). | |
| Authors: | Li Guojuan Liu Jixing Li Baoyu Lou Zhongzi Tian Yongqiang |
| Institution: | Li Guojuan1,2 Liu Jixing1 Li Baoyu1 Lou Zhongzi1 Tian Yongqiang2(1Key Laboratory of Animal Virology of Ministry of Agriculture,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,2College of Chemical , Biological Engineering,Lanzhou Jiaotong University,Lanzhou 730070) |
| Abstract: | The truncated ORF5 gene segments of PRRS which deleted hydrophobic N-terminal sequence,amplified from recombinant plasmids p-GEM-ORF5 and were cloned into prokaryotic expression vector pGEX-4T-1.The recombinant proteins GST-dORF5 were highly expressed in Escherichia coli Rosetta.Western blotting analysis showed the recombinant protein could react with antibody.The result provided fundamental data and materials for the further study on the structure and function of GP5 protein of PRRSV. |
| Keywords: | Porcine reproductive and respiratory syndrome Gene ORF5 Prokaryotic expression |
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