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Changes in the electric characteristics of the inner surface of Escherichia coli cytoplasmic membrane after the binding of microdoses of copper to its outer surface |
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| Authors: | L A Volodina L M Baider A B Shapiro D Sh Burbaev |
| Institution: | (1) Institute of Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskii pr. 38/2, Moscow, 117829, Russia;(2) Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 117334, Russia;(3) Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 117334, Russia |
| Abstract: | The electric characteristics of the surface structures of intact Escherichia coli spheroplasts and the spheroplasts with the outer membrane modified by microdoses of copper were studied by electron spin resonance using the positively charged spin probes of KATn type and the newly synthesized KAT15. The experimental results were analyzed using the Gouy-Chapman formalism. It was found that microdoses of copper ions bound to the outer surface of the cytoplasmic membrane influenced the charge density on the inner surface of the membrane. |
| Keywords: | Escherichia coli spin probes charge density of membranes electron spin resonance |
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