The effect of alkanes on the physiology and metabolism of the entomopathogenic fungus,Isaria fumosorosea |
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| Authors: | Shaukat Ali Muhammad Hamid Bashir Shunxiang Ren |
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| Institution: | 1. Engineering Research Center of Biological Control, Ministry of Education, College of Natural Resource and Environment, South China Agricultural University, Wushan Road, Guangzhou City, China;2. Department of Agricultural Entomology, University of Agriculture, Faisalabad, Pakistan |
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| Abstract: | The influence of different alkanes on spore morphology, glyoxlate pathway enzyme activities, total lipid contents and fatty acid composition of Isaria fumosorosea were investigated under laboratory conditions. Fungal spores grown on different alkanes showed higher germination and mycelial growth when compared to control. A strong induction of glyoxlate cycle enzymes in cell-free extracts was observed for cells grown on different alkanes when compared to glucose and control. Higher activities of glyoxlate cycle enzymes were observed for cells grown on alkanes when compared to other treatments. Even numbered fatty acids accounted for the majority of fatty acid production with a significant increase in relative amounts of linoleic acid and palmatic acid observed for conidia grown on alkanes. These results indicate that addition of alkanes to culture media can be a tool to pre-induce metabolic adaptations that can facilitate successful infection of insect host by entomopathogenic fungi. |
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| Keywords: | entomopathogenic fungi alkane growth fatty acids glyoxylate tricarboxylic acid cycle |
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