Adenovirus fiber protein (FP) functions as a mitogen and an adjuvant |
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Authors: | M Gibson P Tiensiwakul N Khoobyarian |
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Institution: | Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago, Illinois 60680 U.S.A. |
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Abstract: | Fiber protein (FP) from adenovirus serotype 12 and 2 was shown to be mitogenic for lymphocytes of normal BALB/c mice. Maximum increase in 3H]thymidine incorporation was observed with 50–75 μg/ml of adenovirus 12 FP after 48 hr of culture. Also, FP induced blast cell transformation of mouse lymphocytes. The mitogenic activity was abolished with corresponding antiserum. Enriched T cells were not activated by FP, while B cells from athymic nude mice were stimulated to levels of approximately those of whole spleen cells. The stimulatory activity of FP was amplified by the presence of an adherent cell population (probably macrophages). Furthermore, FP served as an adjuvant in vivo, increasing IgM synethesis to SRBCs in mice immunized with FP along with SRBC. The implications of these findings are discussed. |
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Keywords: | To whom reprint requests should be addressed |
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