Linkage analysis of a fertility restoring mutant generated from CMS rice |
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Authors: | Y W Shen Z Q Guan J Lu J Y Zhuang K L Zheng M W Gao X M Wang |
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Institution: | (1) Institute of Genetics, Fudan University, Shanghai 200433, China, CN;(2) China National Rice Research Institute, Hangzhou 310006, China, CN;(3) Institute of Nuclear-Agricultural Sciences, Zhejiang Agricultural University, Hangzhou 310029, China, CN |
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Abstract: | DNA polymorphism between a cytoplasmic male-sterile rice line II-32A, the male-fertile maintainer counterpart II-32B, a fertile
revertant (T24), as well as two commercial indica restorers, was analyzed with randomly amplified polymorphic DNA (RAPD).
A very low degree of polymorphism was found between the revertant T24 and II-32A compared with that of indica rice varieties.
This result, together with agronomic and genetic evidence, suggests the revertant to be a product of a nuclear mutation. An
analysis of polymorphism between II-32A and the revertant T24 with 510 RAPD decamer primers identified the co-segregating
markers OPB07640 and OPB181000 to be linked to a sterile allele of the restoring locus in the revertant T24, at a distance of 5.3 cM. RAPD analysis of a
mapping population of Tesanai2/CB with primer OPB07 revealed linkage of OPB07640 with RG374 (10.8 cM) and RG394 (8.8 cM) on chromosome 1. Thus the restorer gene, designated Rf
5, was tentatively localized between RG374 and RG394 on chromosome 1 and appears to be independent of other mapped restorer
genes in rice.
Received: 11 November 1997 / Accepted: 17 December 1997 |
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Keywords: | Rice (Oryza sativa L) CMS Fertile revertant Restorer gene RAPD marker Mapping |
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