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A new method for producing biologically active nanocomplexes by a noncovalent conjugation of proteins with viral particles |
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| Authors: | A. A. Yaroslavov I. B. Kaplan T. N. Erokhina S. Yu. Morozov A. G. Solovyev A. D. Leshchiner A. A. Rakhnyanskaya A. S. Malinin L. A. Stepanova O. I. Kiselev J. G. Atabekov |
| Affiliation: | 1. Moscow State University, Moscow, 119991, Russia 2. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia 3. Institute of Influenza, Ministry of Public Health and Social Development of the Russian Federation, St. Petersburg, Russia |
| Abstract: | A new method for noncovalent immobilization of a peptide epitope on the virion surface was developed to simplify and standardize the procedures for producing viral nanocomplexes. The efficacy of this approach was demonstrated by the example of a model system comprising the tobacco mosaic virus, synthetic cationic polymer poly-N-ethyl-4-vinylpyridinium bromide, and a model polypeptide. The principle of sequential adsorption, underlying production of the triple system virion-polycation-protein, was used for electrostatic immobilization of a recombinant hydrophilic fragment of the influenza virus hemagglutinin (Flu1-3) on the virion surface. The method provided for a significant increase in the immunogenic activity of this potential artificial vaccine protein. |
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