A novel antifungal analog peptide derived from protaetiamycine |
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Authors: | Juneyoung Lee Hyun Joo Hong Jin-Kyoung Kim Jae-Sam Hwang Yangmee Kim Dong Gun Lee |
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Institution: | (1) College of Agronomy, Jilin Agricultural University, 2888 Xincheng Road, Changchun, 130118, Jilin Province, China |
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Abstract: | Previously, the 9-mer analog peptides, 9Pbw2 and 9Pbw4, were designed based on a defensin-like peptide, protaetiamycine isolated
from Protaetia brevitarsis. In this study, antifungal effects of the analog peptides were investigated. The antifungal susceptibility testing exhibited
that 9Pbw4 contained more potent antifungal activities than 9Pbw2. A PI influx assay confirmed the effects of the analog peptides
and demonstrated that the peptides exerted their activity by a membrane-active mechanism, in an energy-independent manner.
As the noteworthy potency of 9Pbw4, the mechanism(s) of 9Pbw4 were further investigated. The membrane studies, using rhodamine-labeled
giant unilamellar vesicle (GUV) and fluorescein isothiocyanate (FITC)-dextran loaded liposome, suggested that the membrane-active
mechanism of 9Pbw4 could have originated from the poreforming action and the radii of pores was presumed to be anywhere from
1.8 nm to 3.3 nm. These results were confirmed by 3D-flow cytometric contour-plot analysis. The present study suggests a potential
of 9Pbw4 as a novel antifungal peptide. |
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