能化时线粒体内膜脂双分子层结构多相动态

本文报道了鼠肝线粒体内膜体，在琥珀酸氧化或ＡＴＰ水解建立跨膜质子电化学梯度时，膜脂双分子层中ＤＰＨ荧光偏振值出现多个时相动态；ｒ值先迅速下降，再缓慢上升，最终达到高于能化前的ｒ值的恒稳态，表明能化时线粒体内膜脂双分子层有序性在经历短暂下降后，逐渐增大，最终达到更大的结构有序性。在相同时程内呼吸链细胞色素类也经历了相应的多相变化，本文讨论了这两者相关的可能机制，并为呼吸链电子传递机制的动态聚集模型提

Multi-phase Dynamics of the Membrane Lipid Bilayer of Rat Liver Mitoplast Upon Energization

This paper deals with the studies on the relationship of the structural dynamics of lipid bilayer and redox enzyme activities of energized mitoplast membrane.Upon succinate oxidation or ATP hydrolysis by rat liver mitoplast,the time course of changes in fluorescence anisotropy value(r)of DPH incorporated into mitoplast membrane showed a multi-phase dynamies of r value,that is,a rapid phase of r value decrease(fluidity increase)followed by a slow phase of r value increase (fiuidity decrease),and a final steady state phase with much higher r value than that before energization.The changes in redox enzyme activities of the respiratory chain were monitored by both the redox difference spectroscopy and the two vavelength redox difference spectroscopy.A similar pattern of multi-phase dynamics in redox activities of the respiratory chain was also observed and basically synchronized with the multi-phase dynamies of r value of the lipid bilayer.These results imply that the multi-phase dynamics of DPH r value was associated with the respiratory chain activities.A possible explanation for the molecular mechanism of the phenomena observed has been presented.