Polymorphism for PCR-analyzed microsatellites between the inbred mouse strains LG and SM |
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Authors: | E. J. Routman J. M. Cheverud |
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Affiliation: | (1) Department of Anatomy and Neurobiology, Washington University School of Medicine, 63110 St. Louis, Missouri, USA;(2) Present address: Department of Biology, San Francisco State University, 1600 Holloway Avenue, 94132 San Francisco, CA, USA |
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Abstract: | Using agarose gel electrophoresis, we surveyed four strains of inbred mice (AKR/J, C57BL/J, LG/J, and SM/J) for 472 microsatellite loci. Agarose electrophoresis proved to be extremely efficient in separating alleles differing by six or more base pairs and detected a majority of allelic differences of between two and six base pairs. Overall, 64.4% of loci showed polymorphism among the four strains, and pairwise comparisons ranged from 42.1% to 48.1%. Microsatellite polymorphism for strains LG/J and SM/J has not been previously described and was sufficiently high (47.1%) to make these size-divergent strains excellent candidates for quantitative trait loci (QTL) analysis of normal growth. |
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