蔗糖脂肪酸酯对高压静电场与过氧化氢酶作用的影响

研究了蔗糖脂肪酸酯对高压静电场与过氧化氢酶作用的影响 ,结果表明 :在强度为 3× 10 3 v cm的电场作用下 ,蔗糖脂肪酸酯在 3.2mmol L的高浓度以上 ,可以保护过氧化氢酶不受高压静电场的影响。这可能是蔗糖脂肪酸酯使得溶液介电常数增大 ,使得作用在过氧化氢酶分子上的电场强度减小所致 ;在 1.4～ 1.6mmol L的浓度区间中 ,高压静电场使得过氧化氢酶的活力先增加后下降 ;在 0 .4～ 0 .7mmol L时 ,高压静电场使得过氧化氢酶的活力单调下降 ;在0 .0 0 8～ 0 .2 8mmol L的浓度范围内 ,高压静电场使过氧化氢酶的活力先下降后上升 ,可能使酶达到了一种新稳定态。蔗糖脂肪酸酯使得高压静电场对过氧化氢酶的作用变快 ,2～ 4min酶活力开始发生变化 ,而对照需要 2 0min以上。

The Effects of Sucrose Fatty Ester(SFE) on Interaction between High Voltage Electrostatic Field and Catalase

This article studied the effects of SFE on interaction between high voltage electrostatic field and catalase. The results indicate that:at 3×10 3v/cm,SFE concentration is higher than 3.2mmol/L,catalase can be protected by SFE from high voltage electrostatic field,the activity of catalase does not vary. This probably results from the rise of dielectric constant with SFE,because the higher dielectric constant makes the decrease of electric field strength. When the SFE concentration is from 1.4mmol/L to 1.6mmol/L,the activity of catalase increases and then decreases; And 0.4mmol/L to 0.7mmol/L,the activity of catalase decreases only;and 0.008mmol/L to 0.28mmol/L,the activity of catalase decreases and then increases. This is probably for catalase going to a new stable state.