Environment-dependent mechanism of dehalogenation by Rhodococcus chlorophenolicus PCP-1 |
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Authors: | Jussi S Uotila Veikko H Kitunen Juha H A Apajalahti Mirja S Salkinoja-Salonen |
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Institution: | (1) Institute of Applied Chemistry and Microbiology, University of Helsinki, SF-00710 Viikki, Helsinki, Finland;(2) Finnish Forest Research Institute, PL 18, SF-01301 Vantaa, Finland;(3) Technology Centre, Cultor Ltd., SF-02460 Kantvik, Finland |
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Abstract: | Cells and cell-free preparations of a soil-bioremediating organism, Rhodococcus chlorophenolicus PCP-1, dehalogenated polychlorophenols both under aerobic and anaerobic conditions. Under aerobic conditions molecular O2 served as the source of oxygen for the dechlorinating para-hydroxylation reaction. Chlorophenols were dehalogenated and para-hydroxylated also under anaerobic conditions by a cyt P-450 enzyme. Water was used anaerobically as an oxygen source but the reaction required the presence of sulphite ions or iodosobenzene. When the dehalogenating enzyme was given a choice between molecular O2 and water in the presence of sulphite ions or iodosobenzene, the oxygen was preferably derived from water.Dedicated to the honour of Prof. Dr. Norberto Palleroni for the occasion of his 70th birthday
Correspondence to: J. S. Uotila |
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