Construction of a fusion protein carrying antigenic determinants of enteric clostridial toxins |
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Authors: | Belyi Iouri F Varfolomeeva Nina A |
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Affiliation: | Laboratory of Molecular Pathogenesis, Gamaleya Research Institute of Epidemiology and Microbiology, ulitsa Gamalei 18, Moscow 123098, Russia. belyi@riem.ru |
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Abstract: | Clostridium difficile and Clostridium perfringens type A are infectious agents of enteric diseases. The main virulence factors of these microorganisms include toxins A and B of C. difficile (ToxA and ToxB) and enterotoxin of C. perfringens (Cpe). In this study genetic constructions have been created for the expression of ToxA, ToxB and Cpe fragments either as individual components or as a hybrid multidomain (ToxA-ToxB-Cpe) protein. Rabbit monospecific sera raised against individual peptides reacted with the chimeric product indicating that the corresponding antigenic determinants were correctly expressed on the hybrid molecule. Furthermore, mice immunized with the fusion protein produced antibodies specific to each of the three separate components. These data suggest that the constructed three-domain molecule could be used in future studies for development of a vaccine against enteric clostridial diseases. |
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Keywords: | Enteric infection Clostridium Toxin Fusion protein Immunization |
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