全文获取类型
收费全文 | 3686篇 |
免费 | 282篇 |
国内免费 | 181篇 |
专业分类
4149篇 |
出版年
2024年 | 6篇 |
2023年 | 98篇 |
2022年 | 114篇 |
2021年 | 182篇 |
2020年 | 128篇 |
2019年 | 141篇 |
2018年 | 149篇 |
2017年 | 118篇 |
2016年 | 136篇 |
2015年 | 172篇 |
2014年 | 187篇 |
2013年 | 314篇 |
2012年 | 158篇 |
2011年 | 129篇 |
2010年 | 144篇 |
2009年 | 145篇 |
2008年 | 134篇 |
2007年 | 165篇 |
2006年 | 140篇 |
2005年 | 157篇 |
2004年 | 131篇 |
2003年 | 114篇 |
2002年 | 105篇 |
2001年 | 69篇 |
2000年 | 70篇 |
1999年 | 54篇 |
1998年 | 60篇 |
1997年 | 49篇 |
1996年 | 57篇 |
1995年 | 61篇 |
1994年 | 44篇 |
1993年 | 39篇 |
1992年 | 34篇 |
1991年 | 28篇 |
1990年 | 24篇 |
1989年 | 23篇 |
1988年 | 28篇 |
1987年 | 39篇 |
1986年 | 26篇 |
1985年 | 35篇 |
1984年 | 30篇 |
1983年 | 21篇 |
1982年 | 31篇 |
1981年 | 15篇 |
1980年 | 11篇 |
1979年 | 13篇 |
1978年 | 6篇 |
1977年 | 3篇 |
1976年 | 4篇 |
1973年 | 3篇 |
排序方式: 共有4149条查询结果,搜索用时 8 毫秒
1.
Elena C. Guzman Alfonso Jimenez-Sanchez Elisha Orr Robert H. Pritchard 《Molecular & general genetics : MGG》1988,212(2):203-206
Summary A temperature shift-up accompanied by a reduction in RNA polymerase activity in Escherichia coli causes an increased rate of initiation leading to a 1.7- to 2.2-fold increase in chromosome copy number. A temperature shift-up without a reduction in polymerase activity induces only a transient non-scheduled initiation of chromosome replication caused by heat shock with no detectable effect on chromosome copy number. 相似文献
2.
3.
A simple apparatus was developed to allow 12 petri plates to be poured simultaneously by hand. It was used when screening
bacterial isolates from sewage and dog feces for their ability to detect phages from these sources. This was done to assess
the ease with which source-specific phage hosts can be isolated from these sources of fecal pollution. Host bacteria that
consistently detected phages from sewage were easily isolated from sewage. These bacterial isolates did not detect phages
from dog feces. Host bacteria were not isolated from dog feces even after screening hundreds of colonies from fecal samples
from six dogs. Journal of Industrial Microbiology & Biotechnology (2000) 24, 124–126.
Received 06 July 1999/ Accepted in revised form 05 November 1999 相似文献
4.
Rad52 recruitment is DNA replication independent and regulated by Cdc28 and the Mec1 kinase 下载免费PDF全文
Rodney Rothstein 《The EMBO journal》2009,28(8):1121-1130
Recruitment of the homologous recombination machinery to sites of double‐strand breaks is a cell cycle‐regulated event requiring entry into S phase and CDK1 activity. Here, we demonstrate that the central recombination protein, Rad52, forms foci independent of DNA replication, and its recruitment requires B‐type cyclin/CDK1 activity. Induction of the intra‐S‐phase checkpoint by hydroxyurea (HU) inhibits Rad52 focus formation in response to ionizing radiation. This inhibition is dependent upon Mec1/Tel1 kinase activity, as HU‐treated cells form Rad52 foci in the presence of the PI3 kinase inhibitor caffeine. These Rad52 foci colocalize with foci formed by the replication clamp PCNA. These results indicate that Mec1 activity inhibits the recruitment of Rad52 to both sites of DNA damage and stalled replication forks during the intra‐S‐phase checkpoint. We propose that B‐type cyclins promote the recruitment of Rad52 to sites of DNA damage, whereas Mec1 inhibits spurious recombination at stalled replication forks. 相似文献
5.
Natural antibodies to interferon-gamma 总被引:3,自引:0,他引:3
Natural antibodies to interferon (IFN)-γ were detected in the serum of virus-infected patients and also, at a low titre, in
the serum of healthy subjects. The increased titre of antibodies to IFN-γ in the sera of virus-infected patients, and its
decrease with clinical resolution, indicate that these antibodies are related to viral infection and probably reflect IFN-γ
production as a result of antigenic stimulationin vivo. Natural antibodies to IFN-γ were affinity purified and studied for their capability to interferein vitro with the multiple activities of the lymphokine. Data obtained show that these human anti-IFN-γ antibodies have no inhibitory
effect on the antiviral and antiproliferative activity of IFN-γ and do not interfere with the binding of the lymphokine to
its specific cell receptor. Instead, they can inhibit the expression of HLA-DR antigens induced by IFN-γ on U937 cells and
interfere, in mixed lymphocyte culture, with the proliferation of lymphocytes and the generation of cytotoxic lymphocytes.
Experiments in animal models suggest that natural antibodies to IFN-γ may have a role in the immunoregulatory process limiting
the intensity and/or duration of immune response. As they can interfere only with the immunomodulating activities of IFN-γ,
these antibodies might open up new therapeutic approaches to diseases with evidence of activated cell-mediated immunity. 相似文献
6.
E. M. Karalova L. H. Hakobyan M. R. Tatoyan K. T. Sahakyan 《Biotechnic & histochemistry》2013,88(5):309-312
ABSTRACTWe present an easy test for rapid visualization of viral DNA assemblies in infected cell cytoplasm. We selected the best stains for nuclear staining: Nile blue A, Bismarck brown, gallocyanin chrome alum, methyl green pyronin and azure II. None of the staining techniques is fluorescent, which facilitates their use in everyday experiments. Methyl green is most promising for routine detection of viral DNA assemblies in the cytoplasm; the procedure enables ready detection of viral DNA accumulation in the cytoplasm. 相似文献
7.
8.
Prion colonization of secondary lymphoid organs (SLOs) is a critical step preceding neuroinvasion in prion pathogenesis. Follicular dendritic cells (FDCs), which depend on both tumor necrosis factor receptor 1 (TNFR1) and lymphotoxin β receptor (LTβR) signaling for maintenance, are thought to be the primary sites of prion accumulation in SLOs. However, prion titers in RML-infected TNFR1−/− lymph nodes and rates of neuroinvasion in TNFR1−/− mice remain high despite the absence of mature FDCs. Recently, we discovered that TNFR1-independent prion accumulation in lymph nodes relies on LTβR signaling. Loss of LTβR signaling in TNFR1−/− lymph nodes coincided with the de-differentiation of high endothelial venules (HEVs)—the primary sites of lymphocyte entry into lymph nodes. These findings suggest that HEVs are the sites through which prions initially invade lymph nodes from the bloodstream. Identification of HEVs as entry portals for prions clarifies a number of previous observations concerning peripheral prion pathogenesis. However, a number of questions still remain: What is the mechanism by which prions are taken up by HEVs? Which cells are responsible for delivering prions to lymph nodes? Are HEVs the main entry site for prions into lymph nodes or do alternative routes also exist? These questions and others are considered in this article. 相似文献
9.
10.
Summary Irradiation of Escherichia coli with UV light causes a transient inhibition of DNA replication. This effect is generally thought to be accounted for by blockage of the elongation of DNA replication by UV-induced lesions in the DNA (a cis effect). However, by introducing an unirradiated E. coli origin (oriC)-dependent replicon into UV-irradiated cells, we have been able to show that the environment of a UV-irradiated cell inhibits initiation of replication from oriC on a dimer-free replicon. We therefore conclude that UV-irradiation of E. coli leads to a trans-acting inhibition of initiation of replication. The inhibition is transient and does not appear to be an SOS function. 相似文献