Genetic Transformation of Brassica juncea var. tsatsi Induced by Ri T-DNA of Agrobacterium rhizogenes

Hairy roots of mustard (Brassica juncea var. tsatsi) cv. "Paoye' were obtained from in vitro inoculation of reversely inserted petioles with Agrobacteriurn rhizogenes strain LBA9402 harbouring the agropine-type Ri plasmid (pRi1855). The root inducing rate was 100%. Transformed roots grew rapidly on hormone-free MS medium and showed typical hairy root phenotype. Transformed plantlets regenerated from hairy roots on MS medium supplemented with BA 8.0 mg/L and NAA 0.6 mg/L. Opine analysis evidenced the integration and expression of TR-DNA, PCR analysis and Southern hybridization confirmed the integration of TL-DNA including 862 bp rol B sequence in the transformed plants.     

发根农杆菌的Ri T—DNA对茎用芥菜的遗传转化

茎用芥菜品种“泡叶”的倒插叶柄被携农杆碱型Ｒｉ质粒ｐＲｉ１８５５的发根农杆菌菌株ＬＢＡ９４０２感染后获得毛根,出根率为１００％。转化根在不含激素的培养基上快速生长,并表现出典型的生根性状。在含ＢＡ８．０ｍｇ／Ｌ的ＮＡＡ０．６ｍｇ／Ｌ的ＭＳ培养基上毛根再生成完整转化株,冠瘿碱分析表明ＴＲ－ＤＮＡ已在转化株中整合和表达,ＰＣＲ表达和Ｓｏｕｔｈｅｒｎ杂交证实内含８６２ｂｐ ｒｏｌＢ序列的ＴＬ－ＤＮＡ也已