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1.
STEPHEN T. TRUMBO 《Ecological Entomology》2016,41(6):737-740
1. Small, vertebrate carrion is a significant input into terrestrial ecosystems. The relative importance of different recyclers of carrion is poorly understood because of seasonal and habitat variation and methodological differences among studies. Many studies, in particular, do not account adequately for the activity of burying beetles (Nicrophorus spp.). 2. The present work, utilising a previously unstudied woodland, addresses this concern by: placing only two carcasses in the field at one time, using microsites only once, and providing a means to easily recover successful burying beetles. 3. Burying beetles buried 77.5% of experimental mouse carcasses over their breeding season, and typically did so within three activity periods after placement (30 of 31 burials). 4. The results and a brief review suggest that burying beetles are the dominant summer competitor for small, vertebrate carrion in mid‐ and northern latitude woodlands. Information on the age of carcasses utilised by burying beetles also provides an ecological context for laboratory studies of behaviour, life history, and microbial interactions. 相似文献
2.
The multivariate phenetic approach to the classification of Salmo spp. samples from Serbia and adjacent regions of western Balkans for 22 continuous external morphological characters suggests the occurrence of the following distinct stocks: West Danubian (Crno Osoje Stream and upper Zeta River) Salmo taleri , marble trout Salmo marmoratus (Trebuščica River), hatchery-reared Atlantic Salmo trutta , Mlava River drainage (Mlava and Krupaja rivers and Buk Stream) trout Salmo cf. trutta , Velika Morava River system (Godljevača, Bela and Resava rivers) trout S. cf. trutta , Ohrid Lake belvica Salmo ohridana and Aegean coastal drainage Salmo macedonicus (Božica River). In contrast to the phenetic similarity, the phylogenetic reconstruction places the Lake Ohrid belvica as part of an unresolved polytomy with other trout groups. Salmo cf. trutta in the Mlava River appears to form the basal group for the trout species in the region. The position of marble trout implies its independent and more recent origin from the West Danubian trout stock. 相似文献
3.
M. D. Furones † D. J. Alderman D. Bucke T. C. Fletcher ‡§ D. Knox ‡ A. White ‡§ 《Journal of fish biology》1992,41(6):1037-1041
Rainbow trout fed diets containing 7, 86 or 806 mg vitamin E kg−1 for 22 weeks were exposed to virulent Yersinia ruckeri by bath or injection. Mortalities were always least among those fed the highest concentration of vitamin E but serum antibody production was not affected by vitamin E levels. 相似文献
4.
Prey intake by Atlantic salmon Salmo salar and brown trout Salmo trutta was measured across different riparian vegetation types: grassland, open canopy deciduous and closed canopy deciduous, in upland streams in County Mayo, Western Ireland. Fishes were collected by electrofishing while invertebrates were sampled from the benthos using a Surber sampler and drifting invertebrates collected in drift traps. Aquatic invertebrates dominated prey numbers in the diets of 0+ year Atlantic salmon and brown trout and 1+ year Atlantic salmon, whereas terrestrial invertebrates were of greater importance for diets of 1+ and 2+ year brown trout. Terrestrial prey biomass was generally greater than aquatic prey for 1+ and 2+ year brown trout across seasons and riparian types. Prey intake was greatest in spring and summer and least in autumn apart from 2+ year brown trout that sustained feeding into autumn. Total prey numbers captured tended to be greater for all age classes in streams with deciduous riparian canopy. Atlantic salmon consumed more aquatic prey and brown trout more terrestrial prey with an ontogenetic increase in prey species richness and diversity. Atlantic salmon and brown trout diets were most similar in summer. Terrestrial invertebrates provided an important energy subsidy particularly for brown trout. In grassland streams, each fish age class was strongly associated with aquatic, mainly benthic invertebrates. In streams with deciduous riparian canopy cover, diet composition partitioned between conspecifics with older brown trout associated with surface drifting terrestrial invertebrates and older Atlantic salmon associated with aquatic invertebrates with a high drift propensity in the water column and 0+ year fish feeding on benthic aquatic invertebrates. Deciduous riparian canopy cover may therefore facilitate vertical partitioning of feeding position within the water column between sympatric Atlantic salmon and brown trout. Implications for riparian management are discussed. 相似文献
5.
Summer habitat use by sympatric Arctic charr Salvelinus alpinus, young Atlantic salmon Salmo salar and brown trout Salmo trutta was studied by two methods, direct underwater observation and electrofishing, across a range of habitats in two sub-arctic rivers. More Arctic charr and fewer Atlantic salmon parr were observed by electrofishing in comparison to direct underwater observation, perhaps suggesting a more cryptic behaviour by Arctic charr. The three species segregated in habitat use. Arctic charr, as found by direct underwater observation, most frequently used slow (mean ±s .d . water velocity 7·2 ± 16·6 cm s−1) or often stillwater and deep habitats (mean ±s .d . depth 170·1 ± 72·1 cm). The most frequently used mesohabitat type was a pool. Young Atlantic salmon favoured the faster flowing areas (mean ±s .d . water velocity 44·0 ± 16·8 cm s−1 and depth 57·1 ± 19·0 cm), while brown trout occupied intermediate habitats (mean ±s .d . water velocity 33·1 ± 18·6 cm s−1 and depth 50·2 ± 18·0 cm). Niche overlap was considerable. The Arctic charr observed were on average larger (total length) than Atlantic salmon and brown trout (mean ±s .d . 21·9 ± 8·0, 10·2 ± 3·1 and 13·4 ± 4·5 cm). Similar habitat segregation between Atlantic salmon and brown trout was found by electrofishing, but more fishes were observed in shallower habitats. Electrofishing suggested that Arctic charr occupied habitats similar to brown trout. These results, however, are biased because electrofishing was inefficient in the slow-deep habitat favoured by Arctic charr. Habitat use changed between day and night in a similar way for all three species. At night, fishes held positions closer to the bottom than in the day and were more often observed in shallower stream areas mostly with lower water velocities and finer substrata. The observed habitat segregation is probably the result of interference competition, but the influence of innate selective differences needs more study. 相似文献
6.
Culture conditions for arresting and stimulating the proliferation of a rainbow trout fibroblast cell line,RTG-2 总被引:1,自引:0,他引:1
L. E. J. Lee A. Martinez N. C. Bols 《In vitro cellular & developmental biology. Plant》1988,24(8):795-802
Summary Conditions for arresting and stimulating the proliferation of the rainbow trout fibroblast cell line RTG-2 have been examined
and the time course of events after stimulation determined. Quiescent populations were achieved in two ways. Cultures grown
to confluency without a medium change for at least 7 d had fewer than 5% of the cells in S phase and few mitotic figures.
Cultures deprived of serum, which could be done for up to 3 d without a loss in cell number, also achieved quiescence. After
3 d without serum, less than 1% of cells were in S phase and mitotic figures were infrequent. Addition to these cultures of
fresh serum-containing medium brought about the synchronous entry of cells into S phase and mitosis. For cultures in which
either the medium had been changed after 7 d without a change or serum-containing medium had been added after 3 d of serum
deprivation, DNA synthesis increased after a lag period of 20 to 24 h, was pronounced between 30 and 45 h, and then declined.
This was followed by a peak in the mitotic index. These protocols for arresting and subsequently stimulating RTG-2 proliferation
should allow the G1-S transition to be studied in a representative of teleosts.
This research was supported by Natural Sciences and Engineering Research Council of Canada grant to N. C. B. 相似文献
7.
Neuropeptide Y (NPY) has been isolated from brain extracts of the rainbow trout (Oncorhynchus mykiss) and subjected to structural analyses. Plasma desorption mass spectroscopy estimated the molecular mass of the purified peptide as 4303.9 Da. Automated Edman degradation unequivocally established the sequence of a 36 amino acid residue peptide as: Tyr-Pro-Pro-Lys-Pro-Glu-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Pro-Glu-Glu-Leu-Ala-Lys-Tyr-Tyr-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gln-Arg-Tyr. The molecular mass calculated from this sequence (4304 Da) is consistent with that obtained by mass spectroscopy. The presence of a C-terminal amide was established by radioimmunoassay. Rainbow trout NPY is identical in primary structure to coho salmon (Oncorhynchus kisutch) pancreatic polypeptide (PP). These data may indicate that, in this group of salmonid fishes, a single member of the NPY/PP peptide family is expressed in both neurons and peripheral endocrine cells. 相似文献
8.
STEPHEN T. TRUMBO 《Ecological Entomology》1992,17(3):289-298
Abstract.
- 1 To investigate the range of resource size that burying beetles (Nicrophorus) exploit, small (21–33 g), medium (50–90 g) and large (120–210g) carcasses, were placed in the field and then exhumed after 1, 4, 8, 12 or 18 days.
- 2 Nicrophorus attempts to utilize carcasses over this entire size range but has greater success on smaller carcasses.
- 3 Larger carcasses were more difficult to exploit because: (a) they took longer to conceal beneath the leaf litter; (b) they were less likely to be rounded into brood balls; (c) they were more likely to be utilized by dipterans; and (d) they were occupied by greater numbers of congeners.
- 4 Larger carcasses, however, did support greater numbers of larvae and contained broods of greater total mass than smaller carcasses.
- 5 Beetles sometimes bred communally on larger carcasses in the field and same-sex adults were observed to feed young.
- 6 Two follow-up experiments were conducted in the laboratory. On a large carcass N.defodiens, N.tomentosus or N.orbicollis can raise a maximum of 35–50 young. Nicrophorus pustulatus, in contrast, appears to be unique among Nicrophorus in that it can raise nearly 200 young on larger carcasses.
- 7 Nicrophorus orbicollis and Nicrophorus sayi are extremely dependent on parental regurgitations and young fail to survive to the second instar if parents are removed. Young of Nicrophorus defodiens, N.tomentosus and N.pustulatus can develop normally without parental regurgitations.
- 8 I discuss these results in the context of reproduction on carcasses of different size and hypothesize that this breeding system is facultatively quasisocial.
9.
Embryo survival and alevin emergence pattern of brown trout were studied in simulated redds with different homogeneous gravel sizes and different concentrations of peat material. Optimal survival (95%) occurred in 18 mm gravel and survival decreased with decreasing gravel size. High concentrations (40%) of peat material resulted in low survival (65%). The proportion of premature emerging alevins increased in finer gravels and at high peat concentrations. Premature alevins had a large yolk sac and are probably very vulnerable to predators. 相似文献
10.
Michael M. Lipsky Talia R. Sheridan Richard O. Bennett Eric B. May 《In vitro cellular & developmental biology. Plant》1986,22(6):360-362
Summary Comparisons were made of attachment and viability of rainbow trout (Salmo gairdneri) hepatocytes in short-term (2 days), primary culture on plastic, collagen-coated or extracellular matrix (ECM) coated dishes.
Hepatocyte isolation routinely yielded cells with good viability (96%). Cells plated on ECM attached with high efficiency
(93%) in contrast to cells cultured on plastic or collagen (∼20%). The cells plated on ECM flattened out and formed monolayers,
while the cells on plastic and collagen rounded up and formed multi-cell aggregates in suspension. Viability of cells in all
substrates remained high over the 2 day culture period. ECM is the first substrate to support trout-hepatocyte attachment
in primary culture. Differentiated liver function was maintained in cells cultured on ECM as evidence by the induction of
tyrosine aminotransferase by hydrocortisone (200%).
This work was supported in part by research grant R809599010 from the U. S. Environmental Protection Agency.
Editor's Statement This paper reports improved methods for culture of trout liver-derived cells that make in vitro investigations
of fish metabolism, carcinogenesis and chemical toxicity more feasible than previously applied techniques. Recent interest
in fish as models for study and indicators of effects of envionmental and food-related toxins make this work timely, poarticularly
since many of the compounds of interest are primarily metabolized by hepatocytes or act on liver as a major target. David
W. Barnes 相似文献