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A trickle-bed system employing inert matrices of vermiculite or polyurethane foam packed in the downcomer section of a split-flow
air-lift reactor has been developed for hybridoma culture to enhance antibody productivity. This quiescent condition favoured
occlusion and allowed the cells to achieve densities twelve fold greater (12.8×106 cells/ml reactor for polyurethane foam) than in free cell suspension.
The reactor was operated in a cyclic batch mode whereby defined volumes of medium were periodically withdrawn and replaced
with equal volumes of fresh medium. The pH of the medium was used as the indicator of the feeding schedule. Glucose, lactate
and ammonia concentrations reached a stationary value after 5 days. With vermiculite packing, a monoclonal antibody (MAb)
concentration of 2.4 mg/l was achieved after 12 days. The MAb concentration declined then increased to a value of 1.8 mg/l.
In the polyurethane foam average monoclonal antibody (MAb) concentrations reached a stationary value of 1.1 mg/l in the first
20 days and increased to a new stationary state value of 2.1 mg/l for the remainder of the production. MAb productivity in
the trickle-bed reactor was 0.3 mg/l·d (polyurethane foam) and 0.18 mg/l.d (vermiculite) in comparison to 0.12 mg/l·d for
free cell suspension. This trickle-bed system seems to be an attractive way of increasing MAb productivity in culture. 相似文献
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