全文获取类型
收费全文 | 4622篇 |
免费 | 194篇 |
国内免费 | 376篇 |
出版年
2023年 | 30篇 |
2022年 | 30篇 |
2021年 | 59篇 |
2020年 | 69篇 |
2019年 | 66篇 |
2018年 | 84篇 |
2017年 | 61篇 |
2016年 | 83篇 |
2015年 | 83篇 |
2014年 | 132篇 |
2013年 | 230篇 |
2012年 | 147篇 |
2011年 | 156篇 |
2010年 | 128篇 |
2009年 | 180篇 |
2008年 | 190篇 |
2007年 | 186篇 |
2006年 | 263篇 |
2005年 | 292篇 |
2004年 | 277篇 |
2003年 | 294篇 |
2002年 | 273篇 |
2001年 | 255篇 |
2000年 | 188篇 |
1999年 | 155篇 |
1998年 | 167篇 |
1997年 | 140篇 |
1996年 | 148篇 |
1995年 | 107篇 |
1994年 | 130篇 |
1993年 | 110篇 |
1992年 | 76篇 |
1991年 | 71篇 |
1990年 | 41篇 |
1989年 | 27篇 |
1988年 | 45篇 |
1987年 | 29篇 |
1986年 | 23篇 |
1985年 | 21篇 |
1984年 | 15篇 |
1983年 | 11篇 |
1982年 | 17篇 |
1981年 | 17篇 |
1980年 | 12篇 |
1978年 | 8篇 |
1977年 | 10篇 |
1976年 | 11篇 |
1975年 | 9篇 |
1974年 | 10篇 |
1973年 | 13篇 |
排序方式: 共有5192条查询结果,搜索用时 15 毫秒
1.
Disease resistance, stress response and effects of triploidy in growth hormone transgenic coho salmon 总被引:2,自引:0,他引:2
Diploid and triploid coho salmon Oncorhynchus kisutch transgenic for growth hormone (GH) and control coho salmon were compared for differences in disease resistance and stress response. Resistance to the bacterial pathogen Vibrio anguillarum was not affected in transgenic fish relative to their non‐transgenic counterparts when they were infected at the fry stage, but was lower in transgenic fish when infected near smolting. Vaccination against vibriosis provided equal protection to both transgenic and non‐transgenic fish. Triploid fish showed a lower resistance to vibriosis than their diploid counterparts. Diploid transgenic fish and non‐transgenic fish appeared to show similar physiological and cellular stress responses to a heat shock. These studies provide information useful for both performance and ecological risk assessments of growth‐accelerated coho salmon. 相似文献
2.
Jerry W. McClure 《Phytochemistry》1974,13(7):1065-1069
Five-day-old etiolated barley shoots respond to brief illumination with red light by increasing their level of PAL ca 50% within 5 hr. When assayed s 相似文献
3.
4.
The impact of hybrids between genetically modified crop plants and their related species: introgression and weediness 总被引:4,自引:0,他引:4
H. DARMENCY 《Molecular ecology》1994,3(1):37-40
Assessing the impact of hybrids between transgenic plants and nontarget wild species involves answering several questions such as: (i) what are the hybridization and introgression rates; (ii) what is the behaviour of a transgene in a wild population; and (iii) what will be the consequences of the expression of a transgene in a wild population? These issues are discussed using results from experiments on oilseed rape and wild related Brassiceae. Evidence is given of large variations in the estimates of cross-fertilization probabilities. The first stage of introgression into wild populations is demonstrated to occur spontaneously through back-crossing. Population analysis may also be valuable to detect traces of past introgression. Data from the literature on weed biology, and especially herbicide resistance, are used to illustrate the behaviour of a new gene in weed populations. The need for computer models simulating the introgression process is stressed. 相似文献
5.
《Bioscience, biotechnology, and biochemistry》2013,77(12):2762-2765
Comparative two-dimensional electrophoresis showed six proteins, which were significantly produced in the root of salt-tolerant barley. These proteins were identified as stress/defense-related proteins that do not scavenge reactive oxygen species directly, suggesting that salt-tolerant barley develops not only an antioxidative system, but also physical and biochemical changes to cope with salt stress. 相似文献
6.
7.
8.
Emergence and growth of barley was severely decreased by short periods (less than 24 hours) of pre-emergence waterlogging
at 20°C. The extent of damage depended on a combination of duration of waterlogging, soil water potential and aggregate size.
Potentials of less than—4kPa prevented loss of plants developing in aggregates of less than 2 mm diameter after a transitory
period of waterlogging although some shoot and root damage occurred. By comparison seeds growing in soil consisting of aggregates
greater than 2 mm in diameter were not damaged by transitory waterlogging even when drainage only occurred at−0.8kPa. The
severity of damage increased with the period of waterlogging. A criterion obtained as the product of mean size grade and water
potential gave a single value (−4NM−1) below which emergence was satisfactory. Waterlogging halfway through germination gave more severe damage than near sowing
date or near emergence. 相似文献
9.
For three acid soils from Santa Catarina, Brazil, lime application and time of incubation with lime had little effect on the
adsorption of added phosphorus. In two soils with high contents of exchangeable aluminium, solution P and isotopically exchangeable
P were decreased by incubating with lime for 1 month: phosphorus was probably adsorbing on freshly precipitated aluminium
hydrous oxides. In one soil with less exchangeable aluminium, P in solution was increased by liming. After 23 months lime
increased solution and exchangeable P possibly due to crystallization of aluminium hydrous oxides reducing the number of sites
for P adsorption. All these changes were however small.
In a pot experiment, lime and phosphorus markedly increased barley shoot and root dry matter and P uptake. Although liming
reduced P availability measured by solution P, isotopically exchangeable P and resin extractable P, it increased phosphorus
uptake by reducing aluminium toxicity and promoting better root growth. The soil aluminium saturation was reduced by liming,
but the concentration of aluminium in roots changed only slightly. The roots accumulated aluminium without apparently being
damaged. 相似文献
10.
Elizabeth A. Bray Satoshi Naito Nai-Sui Pan Edwin Anderson Philip Dubé Roger N. Beachy 《Planta》1987,172(3):364-370
Soybean (Glycine max (L.) Merr.) seeds contain the storage protein -conglycinin, encoded by a multigene family. -Conglycinin consists of three subunits; , , and . A genomic clone for a -subunit of -conglycinin has been characterized by restriction-enzyme mapping and hybrid selected in-vitro translation followed by immunoprecipitation. In order to determine the developmental regulation of this -subunit gene, its expression was studied in seeds of transgenic petunia (Petunia hybrida) and tobacco (Nicotiana tabacum L.) plants. The -subunit expressed in seeds of petunia and tobacco was recognized by anti--conglycinin serum at a relative molecular mass of 53 000, equivalent to that of the native protein. Separation of the petunia-seed proteins by isoelectric focusing followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analysis showed that multiple isoelectric forms of the -subunit were produced. There was approximately a twofold variation in the accumulation of the -subunit protein in the mature seeds of transgenic petunia plants, each containing a single -subunit gene. However, the level of protein accumulation in mature seeds and the amount of -subunit mRNA in developing seeds was not correlated. Accumulation of the -subunit protein in transgenic seeds was less than the -subunit protein that accumulated in transgenic petunia seeds containing a single -subunit gene and less than the amount of the -subunit in mature soybean seeds which contain 8–13 -subunit genes. In transgenic tobacco plants, the accumulation of the -subunit protein in seeds was generally well correlated with the number of genes that were incorporated in the different transformants.Abbreviations kb
kilobase
- kDa
kilodalton
- Mr
relative molecular mass
- SDS-PAGE
sodium dodecyl sulfate polyacrylamide gel electrophoresis 相似文献