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1.
Carmen de Sena-Tomás Mónica Navarro-González Ursula Kües José Pérez-Martín 《Genetics》2013,195(1):47-57
The fungal fruiting body or mushroom is a multicellular structure essential for sexual reproduction. It is composed of dikaryotic cells that contain one haploid nucleus from each mating partner sharing the same cytoplasm without undergoing nuclear fusion. In the mushroom, the pileus bears the hymenium, a layer of cells that includes the specialized basidia in which nuclear fusion, meiosis, and sporulation occur. Coprinopsis cinerea is a well-known model fungus used to study developmental processes associated with the formation of the fruiting body. Here we describe that knocking down the expression of Atr1 and Chk1, two kinases shown to be involved in the response to DNA damage in a number of eukaryotic organisms, dramatically impairs the ability to develop fruiting bodies in C. cinerea, as well as other developmental decisions such as sclerotia formation. These developmental defects correlated with the impairment in silenced strains to sustain an appropriated dikaryotic cell cycle. Dikaryotic cells in which chk1 or atr1 genes were silenced displayed a higher level of asynchronous mitosis and as a consequence aberrant cells carrying an unbalanced dose of nuclei. Since fruiting body initiation is dependent on the balanced mating-type regulator doses present in the dikaryon, we believe that the observed developmental defects were a consequence of the impaired cell cycle in the dikaryon. Our results suggest a connection between the DNA damage response cascade, cell cycle regulation, and developmental processes in this fungus. 相似文献
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《Saudi Journal of Biological Sciences》2020,27(6):1573-1579
Supplementation of the growing substrate by nitrogenous additives has been known to improve the production of oyster mushroom (Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm. (1871)). However, the application of nano-additives has not been reported in such cultivation yet. The study investigated the effect of nano-urea added in two different doses (3 g and 5 g per kg substrate), once (at spawning or after first flush) or twice (at spawning and after first flush) to the growing substrate consisting of wheat straw and spent oyster substrate (1:1, w/w). Results showed that the application of nano-urea once has induced the highest number of mushroom flushes (four flushes) despite the dose applied. Contrarily to early findings, where high doses of nitrogen have caused inhibition of mushroom growth and production, nano-urea application has had better effects when applied twice. With 5 g/kg, it induced the shortest period between the first and the third flush (15 days). With 3 g/kg, it resulted in the highest biological and economic yields at the third flush (332.7 g/bag and 283.1 g/bag respectively), in total (973.4 g/bag and 854.0 g/bag respectively), the highest biological efficiency (109.6%), and pileus diameter/stipe length ratio (2.8). Experimental findings of the current study may be potentially applied at commercial scale. 相似文献
4.
Rice straw decomposition in rice-field soil 总被引:1,自引:0,他引:1
Rice straw, buried in a rice-field during the dry season decomposed at a rate of 0.0075 day-1. Seventy five percent of the biomass, 70 percent carbon, 50 percent nitrogen and 30 percent phosphorus remained after 139 days of decomposition. Rice straw decomposition furnished 33% N and 8% P of the total nitrogen and phosphorus provided by man. 相似文献
5.
稻草—凤尾菇—蚯蚓—肉鸡食物链的氮流与能流研究 总被引:4,自引:0,他引:4
稻草养菇、菇渣喂蚓、以蚂喂鸡是通过营养关系将三个生产过程有机地连接起来,形成农业生态系统特有的食物链。关于这三个环节各自的经济效益及栽培技术方面的研究工作已经做了很多,而作为一条食物链的整体状况,主要是能流和物流状况。尚未见到更深入具体的报道。作者试图通过实验定量分析和评价该食物链中每一链节上以及整体上的氮流和能流状况,进而对其生产应用价值做一初步探讨。 相似文献
6.
S. G. Nugroho J. Lumbanraja H. Suprapto Sunyoto H. Haraguchi M. Kimura 《Plant and Soil》1996,181(2):287-293
Yearly and seasonal (rainy and dry seasons) variations of CH4 emission from a Sumatra paddy field were measured for 3 years. The mean CH4 emission rates during the growth period were in the range of 16.0–26.1 mg CH4 m-2 h-1 for the chemical fertilizer plots and 23.3–34.9 mg CH4 m-2 h-1 for the plots with rice straw application, respectively. The increase in the amounts of CH4 emission by rice straw application were from 1.3 to 1.6 times. There was no significant difference in the mean CH4 emission rates between rainy and dry seasons.Total amounts of CH4 emitted during the period of rice growth were in the ranges of 29.5–48.2 and 43.0–64.6 g CH4 m-2 for the plots applied with chemical fertilizer and those with rice straw application, respectively. Nearly the same amounts of CH4 were emitted in the first and second half of the growth period, irrespective of rice straw application. 相似文献
7.
Mushroom poisonings caused by amatoxins are mostly lethal. Information about mycetisms caused by white species ofAmanita is scarce. The present paper describes a case of mushroom poisoning caused byA. virosa. A prolongated latency period (6–10 hours), followed by cholera-like, improvement and visceral complication phases confirmed the amatoxin poisoning. The consumption of about 3 pounds of the toadstool by seven persons caused the death of five. Two patients survive the ingestion. 相似文献
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麦秸覆盖夏玉米对其苗期生长发育的生化他感作用研究初报 总被引:8,自引:0,他引:8
麦秸覆盖夏玉米对其苗期生长发育的生化他感作用研究初报张玉铭,马永清(中国科学院石家在农业现代化研究所,050021)AllelopathicEffectofWheatStrawMulchingonSeedlingGrowthandDevelopmen... 相似文献
10.
5mg/L—10mg/L的茶碱在0℃─10℃变温条件下诱导野生毛尖蘑形成了实体原基。分析证明,此条件下毛尖蘑菌丝体呼吸强度提高,体内可溶性蛋白质增加,而DNA与RNA总量却稍有下降之势。本研究为珍品毛尖蘑人工驯化做了先导性工作。 相似文献