全文获取类型
收费全文 | 5778篇 |
免费 | 697篇 |
国内免费 | 1737篇 |
专业分类
8212篇 |
出版年
2024年 | 58篇 |
2023年 | 195篇 |
2022年 | 261篇 |
2021年 | 302篇 |
2020年 | 354篇 |
2019年 | 395篇 |
2018年 | 335篇 |
2017年 | 328篇 |
2016年 | 344篇 |
2015年 | 295篇 |
2014年 | 361篇 |
2013年 | 365篇 |
2012年 | 301篇 |
2011年 | 304篇 |
2010年 | 280篇 |
2009年 | 338篇 |
2008年 | 363篇 |
2007年 | 337篇 |
2006年 | 299篇 |
2005年 | 257篇 |
2004年 | 272篇 |
2003年 | 232篇 |
2002年 | 206篇 |
2001年 | 164篇 |
2000年 | 185篇 |
1999年 | 141篇 |
1998年 | 130篇 |
1997年 | 91篇 |
1996年 | 89篇 |
1995年 | 88篇 |
1994年 | 59篇 |
1993年 | 58篇 |
1992年 | 54篇 |
1991年 | 53篇 |
1990年 | 31篇 |
1989年 | 30篇 |
1988年 | 44篇 |
1987年 | 26篇 |
1986年 | 29篇 |
1985年 | 30篇 |
1984年 | 28篇 |
1983年 | 9篇 |
1982年 | 24篇 |
1981年 | 10篇 |
1980年 | 22篇 |
1979年 | 10篇 |
1978年 | 6篇 |
1977年 | 7篇 |
1976年 | 6篇 |
1973年 | 4篇 |
排序方式: 共有8212条查询结果,搜索用时 0 毫秒
1.
2.
Abstract We have analyzed the sequence downstream of rpoN from Zcinetobacter calcoaceticus and identified an open reading frame encoding a protein with high similarity to UDP- N -acetylgucosamine 1-carboxyvinyl-transferase (MurZ). Multicopy plasmids encoding this enzyme conferred phosphomycin resistance to A. calcoaceticus . The polar effect of a rpoN mutation on the phosphomycin resistance level suggests that murZ is, in part, cotranscribed with rpoN . These observations confirm that A. calcoaceticus represents the first exceptin from a conserved genetic context of rpoN observed in several other Gram-negative bacteria. 相似文献
3.
Surface sediment diatoms from the east coast of Lake Tanganyika were analysed using ordination and classification techniques, and compared with assemblages previously described from the northern part of the lake. Grain-size analyses were performed on subsamples. Four groups of diatom assemblages were recognised. The first group clusters samples taken in the north, far from the Rusizi river mouth. The second group comprises samples taken on silty sediment along the Tanzanian coast, including one sample taken near the mouth of the Malagarazi river and those from the northernmost part of the lake. The third group comprises surface sediments along the Burundian coast (near Ramba and Magara), and the fourth is characterised by epipsammic taxa. A sample taken near the central arm of the Malagarazi river is included in the latter group. The impact of small rivers on the diatom assemblages in the surface sediments is restricted to the mouth area. 相似文献
4.
The morphology of dissociated single cerebellar Purkinje cells obtained from wild-type P1 CD1 mice was assessed in the absence
and in the presence of glia. A dedicated noninvasive technique based on optical microscopy was developed. Image processing
algorithms were implemented to extract metrical features characterizing cell structure and dendritic arborization. The morphological
features were analyzed in order to identify quantitative differences in Purkinje cell morphology due to interactions with
astrocytes. 相似文献
5.
Location within a tree was analyzed as a source of variation in Sequoia sempervirens leaf monoterpenes. No differences were found for quantitative composition or total yield/dry wt among lower, middle and upper canopy positions. The awlshaped, spirally arranged leaves of vigorous upper shoots showed small quantitative compositional differences, but not differences in total yield. The intermediate leaf form of young sprouts had the most different monoterpene quantitative composition and about three times the total yield of the above two leaf forms. Analysis of a clonal ring of 17 adult trees resulted in coefficients of variation similar to those for samples collected from different canopy levels of the same shoot. Results revealed the sources and magnitudes of experimental error in comparative studies of this species' leaf monoterpenes, and did not support the concept that somatic mutation provides an important source of variation in a large, long-lived organism such as coast redwood. 相似文献
6.
7.
8.
The nocturnally active weakly electric fish Gnathonemus petersii is known to employ active electrolocation for the detection of objects and for orientation in its environment. The fish emits pulse‐type electric signals with an electric organ and perceives these signals with more than 3,000 epidermal electroreceptor organs, the mormyromasts, which are distributed over the animal's skin surface. In this study, we measured the metric dimensions of the mormyromasts from different body regions to find structural and functional specialization of the various body parts. We focused on the two foveal regions of G. petersii, which are located at the elongated and movable chin (the Schnauzenorgan; SO) and at the nasal region (NR), the skin region between the mouth and the nares. These two foveal regions were compared to the dorsal part (back) of the fish, which contains typical nonfoveal mormyromasts. While the gross anatomy of the mormyromasts from all skin regions is similar, the metric dimensions of the main substructures differed. The mormyromasts at the SO are the smallest and contain the smallest receptor cells. In addition, the number of receptor cells per organ is lowest at the SO. In contrast, at the back the biggest receptor organs with the highest amount of receptor cells per organ occur. The mormyromasts at the NR are in several respects intermediate between those from the back and the SO. However, mormyromasts at the NR are longer than those at all other skin regions, the canal leading from the receptor pore to the inner chambers were the longest and the overlaying epidermal layers are the thickest. These results show that mormyromasts and the epidermis they are embedded in at both foveal regions differ specifically from those found on the rest of the body. The morphological specializations lead to functional specialization of the two foveae. J. Morphol., 2012. © 2012 Wiley Periodicals, Inc. 相似文献
9.
A nationwide health card recording system for dairy cattle was introduced in Norway in 1975 (the Norwegian Cattle Health Services). The data base holds information on mastitis occurrences on an individual cow basis. A reduction in mastitis frequency across the population is desired, and for this purpose risk factors are investigated. In this paper a Bayesian proportional hazards model is used for modelling the time to first veterinary treatment of clinical mastitis, including both genetic and environmental covariates. Sire effects were modelled as shared random components, and veterinary district was included as an environmental effect with prior spatial smoothing. A non-informative smoothing prior was assumed for the baseline hazard, and Markov chain Monte Carlo methods (MCMC) were used for inference. We propose a new measure of quality for sires, in terms of their posterior probability of being among the, say 10% best sires. The probability is an easily interpretable measure that can be directly used to rank sires. Estimating these complex probabilities is straightforward in an MCMC setting. The results indicate considerable differences between sires with regards to their daughters disease resistance. A regional effect was also discovered with the lowest risk of disease in the south-eastern parts of Norway. 相似文献
10.