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1.
Electrical recording and ultrastructure of stylet penetration by the greenhouse whitefly 总被引:2,自引:0,他引:2
J. A. M. Janssen W. F. Tjallingii J. C. van Lenteren 《Entomologia Experimentalis et Applicata》1989,52(1):69-81
Earlier studies have indicated that interior (physical and/or chemical) properties of a plant may be responsible for feeding-site selection by the greenhouse whitefly, Trialeurodes vaporariorum (Westw.). In order to study the process of feeding-site selection further, stylet-penetration activities and the pathway followed by the stylets in host-plant tissue were investigated using a DC electrical recording method and transmission electron microscopy (TEM). Penetrating whiteflies attached to a gold wire were included in an electrical circuit to record electrical penetration graphs (EPGs). Seven EPG patterns have been distinguished, five of which could be correlated with components of the stylet-penetration process: 1) one with penetration of the leaf surface, 2) one with intercellular penetration and salivary-sheath secretion, 3) one with sieve element penetration and ingestion, 4) one with short penetration of a cell, and 5) one with xylem penetration. The stylet pathway is almost completely intercellular before the phloem is reached and in contrast to aphids, brief symplast punctures are very rare. In general, it takes T. vaporariorum more than half an hour from the start of a penetration to reach a sieve element. Rejection of feeding sites occurs within a few minutes of penetration by adult whiteflies, a time span in which stylets are presumed to penetrate just beyond the epidermis. Properties of the apoplast close to the leaf surface seem therefore to play a major role in feeding-site selection.
Résumé De précédentes études ont montré que les propriétés internes (physiques et/ou chimiques) d'une plante peuvent induire la sélection du site de nutrition de la mouche blanche de serres, Trialeurodes vaporariorum (Westw.). Afin d'étudier plus avant le processus de sélection du site de nutrition, les activités de piqûre et le chemin suivi par le stylet dans les tissus de la plante-hôte ont été étudiés par une méthode d'enregistrement électrique en courant continu ainsi que par microscopie à transmission d'électrons (TEM). Des aleurodes en activité de piqûre attachées à un fil d'or ont été incluses dans un circuit électrique pour enregistrer des graphes de pénétration électriques (EPG). Sept motifs d'EPG ont été distingués, dont cinq peuvent être corrélés aux composantes du processus de pénétration du stylet: 1) un avec pénétration de la surface foliaire, 2) un avec pénétration interecellulaire et sécrétion d'une gaine de salive, 3) un avec pénétration du phloème, 4) un avec courte pénétration d'une cellule, et 5) un avec pénétration du xylème. Le parcours du stylet est presque entièrement intercellulaire avant que le phloème soit atteint. Contrairement aux pucerons, les ponctions brèves dans le symplasme sont rares. Généralement, T. vaporariorum met plus d'une demi-heure, à partir du début d'une piqûre, pour atteindre un vaisseau. Le rejet des sites de nutrition par les aleurodes adultes se passe quelques minutes après la pénétration du stylet; pendant ce laps de temps, le stylet pénétrerait juste sous l'épiderme. Le rôle des propriétés de l'apoplaste près de la surface foliaire semble donc majeur dans la sélection des sites de nutrition.相似文献
2.
Biological control of thrips [Thysanoptera: Thripidae] on greenhouse cucumber byAmblyseius cucumeris
D. R. Gillespie 《BioControl》1989,34(2):185-192
The predatory miteAmblyseius cucumeris (Oudemans) [Acarina: Phytoseiidae] was evaluated as a biological control forThrips tabaci Lindeman andFrankliniella occidentalis (Pergande) [Thysanoptera: Thripidae] on greenhouse grown seedless cucumber.A. cucumeris spread throughout the greenhouses and provided control of both species of thrips.A. cucumeris adults persisted on plants for 7 weeks in the virtual absence of prey, and increased numerically in response to increases
in prey population. On the basis of these resultsA. cucumeris is recommended as a useful biological control forT. tabaci andF. occidentalis on greenhouse cucumber.
Publication No. 361, Agassiz Research Station, Agriculture Canada, Agassiz, B.C. Canada VOM 1AO. 相似文献
3.
Somaclonal variation as a tool to develop pest resistant plants of Torenia fournieri ‘Compacta Blue’
Callus cultures of Torenia fournieri Compacta Blue were initiated on a modified Murashige and Skoog salt medium (MS) with 2.26 M 2,4-dichlorophenoxyacetic acid. Shoots were regenerated from these cultures using MS medium amended with 2.46 M indolebutyric acid and 8.88 M benzyladenine. These shoot cultures were subjected to two-spotted spidermite (Tetranychus urticae Koch.) and the greenhouse whitefly [Trialeurodes vaporariorum (Westwood)]. Pests were allowed to feed until such time that their populations started to decrease due to lack of food. The remaining live tissue of the Torenia was placed on MS medium amended with 2.28 M zeatin to induce new adventitious shoots and plantlets. Newly regenerated plantlets were acclimated to greenhouse conditions and evaluated for resistance to the pest to which they were subjected in vitro. Highly significant differences in pest numbers were found in somaclones for both the two-spotted spidermite and greenhouse whitefly when compared to control plants. A wide range of variability was observed among the somaclonal population. There were significantly fewer mite eggs laid on plants regenerated from in vitro cultures screened with two-spotted spidermites than on seed sown controls. Regenerants from cultures screened with whiteflies in vitro had fewer eggs, immatures and live adults than controls.Abbreviations BA
benzyladenine
- IBA
indolebutyric acid
- 2,4-d
2,4-dichlorophenoxyacetic acid
- MS
Murashige and Skoog salt medium
Storrs Agricultural Research Station Scientific Publication 1641. 相似文献
4.
Acclimation of photosynthesis to increasing atmospheric CO2: The gas exchange perspective 总被引:9,自引:0,他引:9
Rowan F. Sage 《Photosynthesis research》1994,39(3):351-368
The nature of photosynthetic acclimation to elevated CO2 is evaluated from the results of over 40 studies focusing on the effect of long-term CO2 enrichment on the short-term response of photosynthesis to intercellular CO2 (the A/Ci response). The effect of CO2 enrichment on the A/Ci response was dependent on growth conditions, with plants grown in small pots (< 5 L) or low nutrients usually exhibiting a reduction of A at a given Ci, while plants grown without nutrient deficiency in large pots or in the field tended to exhibit either little reduction or an enhancement of A at a given Ci following a doubling or tripling of atmospheric CO2 during growth. Using theoretical interpretations of A/Ci curves to assess acclimation, it was found that when pot size or nutrient deficiency was not a factor, changes in the shape of A/Ci curves which are indicative of a reallocation of resources within the photosynthetic apparatus typically were not observed. Long-term CO2 enrichment usually had little effect or increased the value of A at all Ci. However, a minority of species grown at elevated CO2 exhibited gas exchange responses indicative of a reduced amount of Rubisco and an enhanced capacity to metabolize photosynthetic products. This type of response was considered beneficial because it enhanced both photosynthetic capacity at high CO2 and reduced resource investment in excessive Rubisco capacity. The ratio of intercellular to ambient CO2 (the Ci/Ca ratio) was used to evaluate stomatal acclimation. Except under water and humidity stress, Ci/Ca exhibited no consistent change in a variety of C3 species, indicating no stomatal acclimation. Under drought or humidity stress, Ci/Ca declined in high-CO2 grown plants, indicating stomata will become more conservative during stress episodes in future high CO2 environments.Abbreviations A
net CO2 assimilation rate
- Ci (Ca)
intercellular (ambient) partial pressure of CO2
- operational Ci
intercellular partial pressure of CO2 at a given ambient partial pressure of CO2
- gs
stomatal conductance
- normal CO2
current atmospheric mole fraction of CO2 (330 to 355 mol mol–1)
- Rubisco
ribulose-1,5-bisphosphate carboxylase/oxygenase 相似文献
5.
Blue light controls solar tracking by flowers of an alpine plant 总被引:2,自引:0,他引:2
In at least 18 plant families, leaves or flowers can maintain a specific orientation with respect to diurnal movements of the sun. Previous work on heliotropic leaves has demonstrated that blue light (400–500nm) provides the cue for their tracking response. Floral heliotropism occurs in several families of arctic and alpine plants, but the spectral sensitivity of the response has not been studied previously. Moreover, no studies on the spectral sensitivity of any heliotropism have been conducted on wild plants growing in their natural habitat. Working under field conditions, we used coloured acrylic filters to determine whether heliotropism by flowers of the snow buttercup (Ranunculus adoneus) is responsive to broad-band blue or red light. Flowers were able to orient towards the sun under boxes made entirely of blue-transmitting filters and in red-transmitting boxes having a single blue side that faced the sun. In these treatments, solar tracking ability was not significantly different from that observed in adjacent control flowers. In contrast, the precision of solar orientation was significantly reduced in red-transmitting boxes and red boxes with a single blue side oriented away from the sun. In the early morning, flowers covered by red-transmitting boxes failed to orient in the direction of sunrise, suggesting that this floral response, unlike that seen in some heliotropic leaves, lacks a residual‘memory’ for previous solar movements. 相似文献
6.
Xiaoming Li Xiaopeng Duan Jiawei Qiao Shilin Li Yunhao Cai Jianqi Zhang Yuan Zhang Xiaotao Hao Yanming Sun 《Liver Transplantation》2023,13(1):2203044
The power conversion efficiencies (PCEs) of all-polymer solar cells (all-PSCs) have already exceeded 17%. However, the limited absorption range of an all-polymer system results in significantly reduced short-circuit current density (Jsc), which eventually influences the PCE improvement. To broaden the light absorption of polymer acceptors, herein, benzotriazole is introduced in the core unit of small molecule acceptors and thus two narrow-bandgap polymer acceptors named PTz-BO and PTz-C11 featuring the same molecular backbone and different side-chain length are synthesized. Compared with PTz-C11, the PTz-BO based-all PSCs deliver a slightly reduced Jsc, a large open-circuit voltage (Voc) and a low voltage loss below 0.50 V. Moreover, ternary all-PSCs are constructed by introducing PTz-C11 as a guest component. Benefiting from the reduced recombination, improved exciton generation and dissociation, and balanced charge transport, a high efficiency of 16.58% is obtained for the ternary all-PSCs, with a high Jsc over 25 mA cm−2 without sacrificing the Voc. Such result represents the highest efficiency reported for benzotriazole-based all-PSCs in the literature thus far. This work demonstrates the great potential of benzotriazole for the synthesis of efficient narrow-bandgap polymer acceptors. 相似文献
7.
Atlantic salmon survival in the R. Bush (N. Ireland) from egg to summer 0+ was inversely density-dependent on egg deposition ( P <0.05). A stock-recruitment relationship derived from egg deposition and summer 0+ abundance index data was compared to that derived from adult and smolt counts based on total trapping. Fitted Ricker curves indicated maximum recruitment at around 2.35 million eggs and 2.46 million eggs for 0+ index and smolt count methods, respectively. Salmon 0+ abundance index data from semi-quantitative electrofishing could be obtained with relatively little effort, and used to derive whole-river stock-recruitment relationships on rivers where only adult count or some other estimator of parental stock is available. The derivation and expression of spawning targets from stock/recruitment relationships is discussed with reference to the R. Bush data. 相似文献
8.
Yvonne M. van Houten Pam van Stratum Jan Bruin Alfred Veerman 《Entomologia Experimentalis et Applicata》1995,77(3):289-295
In Europe and North America the western flower thrips,Frankliniella occidentalis, is an important pest in various greenhouse crops, such as sweet pepper and cucumber. Two species of predatory mite are commercially
applied for biological control of this pest:Amblyseius cucumeris andA. barkeri. Thrips control is generally successful from March onwards. During winter, however, thrips control by these predatory mites
is less effective. An important reason for this is that the commercially applied strains of both mite species enter reproductive
diapause under short-day photoperiods, whereas the western flower thrips does not enter diapause. In this paper we report
on selection experiments for non-diapause in strains of both mite species, aimed at obtaining predators that do not enter
diapause under light- and temperature conditions prevailing in winter. Additional experiments were done to estimate the potential
of the selected lines as control agents ofF. occidentalis. Selection for non-diapause proved highly successful in both predatory mite species. In a New Zealand strain ofA. cucumeris diapause incidence decreased from 41% to 0% in about ten generations; in a Dutch strain ofA. barkeri diapause incidence decreased from 67% to 0% in about six generations. Furthermore, selection for non-diapause had no influence
on predator performance, measured as predation rate and oviposition rate on a diet of first instar thirps larvae. Rates of
predation and oviposition were the same for selected and unselected lines in both species; rates of predation and oviposition
were higher forA. cucumeris than forA. barkeri. After 18 months under non-diapause conditions, no less than 92% of a sample of the selected non-diapause line ofA. cucumeris did not enter diapause when tested under diapause-inducing conditions. This indicates that ‘non-diapause’ is a stable trait
in these predatory mites. Finally, a small-scale greenhouse experiment in a sweet pepper crop showed that the selected non-diapause
line ofA. cucumeris established successfully under diapause-inducing short-day conditions. 相似文献
9.
10.
This article examines an important class of information system that serves as the foundation for corporate energy and greenhouse gas (GHG) accounting: energy and carbon management systems (ECMS). Investors, regulators, customers, and employees increasingly demand that organizations provide information about their organizational energy use and GHG emissions. However, there is little transparency about how organizations use ECMS to meet such demands. To shed light on ECMS implementation and application, we collected extensive qualitative interview data from two service‐sector organizations: one that uses a spreadsheet‐based ECMS and another that implemented an ECMS provided by a third‐party vendor. Our analysis of collected data revealed numerous challenges in the areas of business processes, managerial capabilities, data capture and integration, and data quality. Though our study is built on only two organizations and requires confirmation in large‐sample surveys, we provide several recommendations for organizations regarding ECMS. We also provide suggestions for future studies to build on our tentative results. 相似文献