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排序方式: 共有567条查询结果,搜索用时 0 毫秒
1.
J.J. Virgen-Ortíz V. Ibarra-Junquera P. Escalante-Minakata J.A. Osuna-Castro J. de J. Ornelas-Paz N.A. Mancilla-Margalli R.L. Castañeda-Aguilar 《Analytical biochemistry》2013
This work presents a rapid and simple freeze centrifugation method to concentrate dilute protein solutions for detection by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) Coomassie blue staining. Moreover, a simple way to assemble a cryoconcentration device is presented, and its use is discussed. Commercial purified protein standard and an enzyme with high fructosyltransferase (FTase) activity, coming from target fractions obtained by chromatographic separation, were used as an example. FTase, coming directly from the chromatographic fractions, was difficult to view through SDS–PAGE analysis; however, it was easily visualized, and its activity was enhanced, after the application of the freeze centrifugation protocol presented here. 相似文献
2.
R Schmutzler J F van Uem K Elkind-Hirsch D Hahn J L McGuire D R Danforth G D Hodgen 《Journal of medical primatology》1988,17(5):281-285
Preliminary data indicate the potential utility of an implantable subcutaneous device that facilitates chronic intravenous infusion of pulsatile gonadotropin-releasing hormone (GnRH) for ovulation induction. GnRH distribution curves were congruent in control monkeys and those with implanted devices. Tissue tolerance was good in this brief trial. These findings suggest that use of this or a similar implantable device be considered for chronic GnRH administration in human pulse therapy. 相似文献
3.
We designed an Integrated Media Preparation System (IMPS) for continuous, on-line preparation of cell culture media and delivery to intermediate storage vessels or directly to a bioreactor. Key components of the IMPS include: a high precision, continuous fluid mixing device; formulation-specific liquid medium concentrates; validated process controls and membrane filtration; and automated dispensing into large volume flexible plastic containers. The IMPS system is designed to produce sterile, single-strength liquid medium from common raw materials at a delivery rate of 1000–3000 liters per hour and will manufacture homogenous batches from several thousand liters to over 60,000 liters. Fortified nutrient media prepared from multi-component 50X concentrates have been demonstrated to accelerate bioreactor seed chains, increase product yield, and reduce the overall manufacturing cost of nutrient medium. A productivity matrix will analyze the fully-loaded costs and contrast alternative methods for media preparation against projected biological yield.Abbreviations IMPS
Integrated Media Preparation System
- 50X
Nutrient fluid components formulated at fifty-fold final use concentration
- 1X
Nutrient fluid formulated at final, single-strength use concentration
- cGMP
Current Good Manufacturing Practices
- SCADA
Supervisory Control and Data Acquisition
- PLC
Process Logic Controller
- LTI
Life Technologies, Inc.
- WFI
Water for Injection
- CIP
Clean in place
- SIP
Sterilize in place
- HPLC
High performance liquid chromatography
- DMEM
Dulbecco's Modified Eagle's Medium 相似文献
4.
热带林茎流收集及计算方法探索 总被引:5,自引:0,他引:5
热带林茎流收集及计算方法探索周光益,吴仲民,李意德,陈步峰(中国林科院热带林业研究所,广州510520)CollectionandCalculationMethodsforStemflowinTropicalForest.¥ZhouGuangyi;W... 相似文献
5.
Wang Zheng-yi 《Biotechnic & histochemistry》1993,68(1):38-41
A new antiroll device has been developed to replace the antiroll guide plate for cryostat wax sectioning. With this device, a continuous ribbon of 3-4 μm sections can be obtained. The sections are flat, uncreased. and compression is reduced to a minimum. 相似文献
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7.
Activities of Phosphorylase, glyceraldehyde-3 -phosphate dehydrogenase, lactate dehydrogenase, malate dehydrogenase and succinate
dehydrogenase in the rat endometrial tissue are significantly inhibited by an intrauterine copper device, while it stimulated
glucose-6-phosphate dehydrogenase activity. The copper device decreased the lactate/pyruvate ratio in the tissue; pyruvate
utilizationin vitro by the rat endometrium is also blocked by copper. These findings suggested that the normal carbohydrate metabolism of the
tissue may be affected in presence of copper, thus resulting in a change of the endometrial function, which may be one of
the factors responsible for the contraceptive and pharmacological action of an intrauterine copper device. 相似文献
8.
9.
Joey O Brien Declan Browne Des Earls Clare Lodge 《Biology of sport / Institute of Sport》2022,39(3):499
The aim of this study was to investigate the effect of four different inertial loads (0.025, 0.050, 0.075, and 0.100 kg· m²) on concentric (CON) power, eccentric (ECC) power, and ECC overload in the flywheel Romanian deadlift (RDL). Fourteen recreationally trained males (27.9 ± 6.4 years, 90 ± 10.7 kg, 180.7 ± 5.5 cm) volunteered for the study. They had a minimum of two years of resistance training experience, although none had experience in flywheel inertia training (FIT). All participants performed the flywheel RDL on a flywheel device (kBox 3, Exxentric, AB TM, Bromma, Sweden). Each set was performed using different inertial loads, those being 0.025, 0.050, 0.075, and 0.100 kg·m². For CON, ECC power, and ECC overload, there was a significant difference (p < 0.001) between inertial loadings. In conclusion, results highlight that lower inertial load leads to higher peak CON and ECC power values, precisely 0.025 kg· m². Regarding ECC overload, medium to higher loads (0.050, 0.075, and 0.100 kg·m²) will lead to higher values. 相似文献
10.