日本血吸虫中国大陆株基因多态性研究

对日本血吸虫中国大陆株湖南、湖北、江西、安徽、四川、云南隔离群以及一个实验室传代品系从基因水平进行了多态性研究。首先,在用ＰＣＲ－ＳＳＷＣＰ技术分析了２８Ｓ ｒＤＮＡ－Ｄ２高变区基础上,测定了该区安徽和云南隔离群的ＤＮＡ序列;其次,用ＰＣＲ获得了含有ＩＴＳ的ｒＤＮＡ片段,并对其进行了酶切点重复序列的多态性分析;最后,用ＲＡＰＤ技术分析了全基因组ＤＮＡ的多态性。结果表明,安徽与云南隔离群的２８Ｓ ｒ     

Effect of environmental factors on Fonsecaea pedrosoi morphogenesis with emphasis on sclerotic cells induced by propranolol

The influence of growth conditions, as well as of propranolol on Fonsecaea pedrosoi morphogenesis was established using the chemically defined media of Czapeck-Dox (CD) and Butterfield (BF). Mycelial growth of F. pedrosoi in both media was obtained at room temperature (25 °C) for 14 days, without shaking, whereas conidia formed at 37 °C, for 4 days, in shaken cultures and could be isolated free from the mycelium by filtration in gauze. At low pH (2.5–3.0), there appeared sclerotic cells attached to normal hyphae. When propranolol was added to the CD medium moniliform hyphae were observed, whereas this drug in the BF medium induced formation of sclerotic cells. Ultrastructural examination revealed that the propranolol-induced sclerotic cells were very similar to those observed in infected tissues.