次氯酸钠离析法观测甘蓝叶片脉序的处理条件优化筛选

次氯酸钠（NaClO）离析法主要用于植物叶片表皮的观测,在研究过程中发现该法也可用于叶片脉序的观测。以甘蓝（Brassica oleracea var.capitata）为实验材料,采用二次正交设计方法对水煮时间、NaOH浓度、NaOH处理时间、NaClO浓度、NaClO处理温度和处理时间等各种处理条件进行优化筛选,以期得到适合于甘蓝叶片脉序观测的最佳处理条件组合。实验结果表明,新鲜甘蓝叶片水煮3分钟,10%的NaOH溶液60°C水浴处理2.5小时,3%的NaClO溶液40°C水浴离析2小时,叶片脉序的观测效果最佳。     

次氯酸钠离析法观测甘蓝叶片脉序的处理条件优化筛选

次氯酸钠(NaClO)离析法主要用于植物叶片表皮的观测, 在研究过程中发现该法也可用于叶片脉序的观测。以甘蓝(Brassica oleracea var. capitata)为实验材料, 采用二次正交设计方法对水煮时间、NaOH浓度、NaOH处理时间、NaClO浓度、NaClO处理温度和处理时间等各种处理条件进行优化筛选, 以期得到适合于甘蓝叶片脉序观测的最佳处理条件组合。实验结果表明, 新鲜甘蓝叶片水煮3分钟, 10%的NaOH溶液60°C水浴处理2.5小时, 3%的NaClO溶液40°C水浴离析2小时,叶片脉序的观测效果最佳。     

Autres applications de la MIBG : « applications de la scintigraphie de l’innervation myocardique à la 123-I-MIBG en neurologie »

Myocardial 123-I-metaiodobenzylguanidine (MIBG) imaging enables the assessment of postganglionic sympathetic cardiac innervation. Recently myocardial 123-I-MIBG scintigraphy has been found to be useful to distinguishing Parkinsońs disease (PD) from other akinetic rigid syndromes. The absence of published guidelines for cardiac 123-I-MIBG imaging makes standardization of procedures among individual users of this technique even more important. Cardiac MIBG study showed markedly decreased uptake in PD, dementia with Lewy bodies (DLB) and pure autonomic failure, which is a common feature of Lewy body disease. The cardiac MIBG uptake is severely impaired even in the early disease stage. Atypical parkinsonian syndromes, including Multiple System Atrophy, Progressive Supranuclear Palsy, and other, show modest reduction of cardiac MIBG uptake. The MIBG study can be used for differentiating patients with extrapyramidal signs and dementia, and might provide useful diagnostic information to discriminate DLB and Alzheime?s disease.     

Bone scintigraphy for cardiac amyloidosis imaging: Past,present and future

The aim of the article was to review the history of the emergence of ^99mTc-labeled phosphate agents in the exploration of cardiac amyloidosis and the give an overview of the technique, its accuracy and its application in clinical practice. Potential future directions are mentioned.     

A rapid protocol to evaluate coronary flow reserve with myocardial scintigraphy: A prospective study using Regadenoson

Cardiac positron emission tomography (PET) is superior to single-photon emission computed tomography (SPECT) thanks to its quantitative analysis capabilities, especially for measuring myocardial blood flow (MBF) and myocardial flow reserve (MFR). The recent development of new cadmium zinc telluride (CZT) gamma cameras has enabled dynamic acquisition, allowing measurement of MBF and MFR. This prospective study aimed to determine the fastest protocol, to analyse MBF and perfusion imaging.Methods and resultsFrom February to June 2018, 10 patients were referred to our centre and prospectively enrolled in our study to evaluate the MBF and MFR, with 5 different reconstruction list-mod. The primary endpoint was to assess the reproducibility of the different reconstruction, and time of acquisition. The secondary endpoint was the evaluation of the image quality of the MPI. The 6-min tests highlighted no significant difference in the flow measurement and MFR (P = 0,15). Differences become significant by decreasing acquisition time. The image quality did not differ significantly (P = 0.6).ConclusionWe determined a fast (12mn acquisition) reliable and reproducible protocol to evaluate MBF and MFR without any loss of MPI information.     

Cell therapy of heart failure

Together with angiogenesis and gene therapy, cell transplantation is one of the newest treatments that have been proposed to improve the still grim outcome of patients with cardiac failure. The underlying rationale is that implantation of contractile cells into fibrous post-infarction scar can functionally 'regenerate' these areas. Primarily for practical reasons, autologous skeletal myoblasts have been the first to be tested in a clinical trial but other cell types can be considered, among which bone marrow stromal and hematopoietic stem cells are of particular interest because of their autologous origin and their purported transdifferentiation potential into cardiac and/or endothelial cells. However, several key issues still need to be addressed, including (i) the optimal type of donor cells, (ii) the mechanism by which cell engraftment improves cardiac function, actively (i.e., by increasing contractility) or passively (i.e. by limiting infarct expansion and remodelling), (iii) the optimisation of cell survival, and (iiii) the potential benefits of cell transplantation in non-ischaemic heart failure. Parallel to the numerous experimental studies designed to address these issues, initial clinical trials are underway or in preparation and, if properly designed and conducted, should allow to know whether the hopes raised by cellular therapy are met by clinically meaningful improvements in the outcomes of patients with heart failure.     

Variations cytologiques des corps allates au cours du cycle reproducteur du Collembole Folsomia candida

In the parthenogenetic females of the Collembola, Folsomia candida, study of the nucleoplasmic ratio and fine structure of the corpora allata cells reveals an activity cycle synchronized with the ovarian cycle. The maximum cytoplasmic volume, connected with a relatively great development of the endoplasmic reticulum, is reached during the vitellogenic phase.The simultaneous control of the ovary by moulting hormones and by a corpus allatum hormone is discussed.