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1.
Extracellular matrix (ECM) modulates the EGF-induced migration of liver epithelial cells in serum-free,hormone-supplemented medium 总被引:2,自引:0,他引:2
Summary The influence of the extracellular matrix (ECM) glycoproteins collagen, IV laminin (LN), and fibronectin (FN) on the in vitro
migration of epithelial cells was studied using the ECM migration track method (4) with preparations immunostained for LN
and FN. The locomotion of rat liver epithelial cells stimulated to migrate in serum-free medium by epidermal growth factor
(EGF) in the presence of the protein per cm2. Neither LN nor collagen IV decreased the number of migrating cells, indicating that the inhibition is a specific effect
of fibronectin. The data also indicate that the FN-mediated inhibition of migration is an additional and not alternative mechanism
to the well-established contact inhibition of locomotion (1) which also occurs in liver epithelial cell cultures. The system
is being used for a further analysis of the factors that influence migration of normal and neoplastic epithelial cells and
the biochemical mechanisms underlying the migration reaction.
Editor’s Statement This paper describes new and heretofore neglected aspects of EGF and fibronectin action on the migratory
behavior of cultured cells. Gordon H. Sato 相似文献
2.
3.
Gintaras Malmiga Maja Tarka Thomas Alerstam Bengt Hansson Dennis Hasselquist 《Journal of avian biology》2021,52(1)
A wide variety of the barrier crossing strategies exist among migrating songbirds, ranging from strict nocturnal flights to non‐stop flights over a few days. We evaluate barrier crossing strategies in a nocturnally migrating songbird crossing the Mediterranean Sea and the Sahara Desert, the great reed warbler, exploring variation between the sexes and within individuals. We used data from 31 year‐round light‐level geolocators tracks from 26 individuals (13 males and 13 females), with four individuals tracked for 2–3.5 consecutive years. Almost all individuals (25 of 26) prolonged their flights into the day at least on one occasion. The mean duration of these prolonged flights was 19.9 h and did not differ between sexes or seasons. Fifteen birds performed non‐stop flights during more than one full day and night (≥ 24 h; mean = 31.9 h; max = 55 h) in autumn and/or spring, but these flights were generally too short to cross an entire barrier (such as the Sahara Desert) in one non‐stop flight. Patterns of prolonged flights showed considerable within‐individual variation in females between seasons (autumn versus spring) and in both males and females between years, suggesting high individual flexibility in migration strategy. Significantly more males than females performed prolonged flights during autumn migration, but not spring, possibly reflecting sex‐specific carry‐over effects. We conclude that great reed warblers have the ability to conduct prolonged continuous flights for up to several nights and days, which potentially would allow them to cross the Sahara Desert in one non‐stop flight. However, they typically use a mixed strategy of several nocturnal flights with intermittent stopovers in combination with 1–3 prolonged flights. Prolonged flights covered less than half (44%) of the total flight time across the barriers, and the diurnal parts of the flights covered only 18% of this time. 相似文献
4.
Erik M. Solhaug Rahul Roy Rodney T. Venterea Clay J. Carter 《The Plant journal : for cell and molecular biology》2021,105(3):580-599
Floral nectar is a sugary solution produced by nectaries to attract and reward pollinators. Nectar metabolites, such as sugars, are synthesized within the nectary during secretion from both pre-stored and direct phloem-derived precursors. In addition to sugars, nectars contain nitrogenous compounds such as amino acids; however, little is known about the role(s) of nitrogen (N) compounds in nectary function. In this study, we investigated N metabolism in Cucurbita pepo (squash) floral nectaries in order to understand how various N-containing compounds are produced and determine the role of N metabolism in nectar secretion. The expression and activity of key enzymes involved in primary N assimilation, including nitrate reductase (NR) and alanine aminotransferase (AlaAT), were induced during secretion in C. pepo nectaries. Alanine (Ala) accumulated to about 35% of total amino acids in nectaries and nectar during peak secretion; however, alteration of vascular nitrate supply had no impact on Ala accumulation during secretion, suggesting that nectar(y) amino acids are produced by precursors other than nitrate. In addition, nitric oxide (NO) is produced from nitrate and nitrite, at least partially by NR, in nectaries and nectar. Hypoxia-related processes are induced in nectaries during secretion, including lactic acid and ethanolic fermentation. Finally, treatments that alter nitrate supply affect levels of hypoxic metabolites, nectar volume and nectar sugar composition. The induction of N metabolism in C. pepo nectaries thus plays an important role in the synthesis and secretion of nectar sugar. 相似文献
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6.
This study investigated the effect of adult associated cues of the multicolored Asian ladybird Harmonia axyridis Pallas (Coleoptera: Coccinellidae) on the foraging behavior of the aphid parasitoid, Aphidius gifuensis Ashmead (Hymenoptera: Aphididae) with three assays: 1) whole aphid leaf-disc with H. axyridis tracks; 2) aphid leaf-disc with only half contaminated with H. axyridis tracks; 3) aphid leaf-discs without H. axyridis tracks. Foraging behavior of A. gifuensis was recorded using the Observer® XT 11 and EthoVsion® XT 12. In addition, functional responses of A. gifuensis in patches with or without H. axyridis cues were also tested. Aphidius gifuensis females preferred oviposition in arenas where no adult tracks of H. axyridis were present. However, no significant difference between functional responses of A. gifuensis foraging in plants with and without H. axyridis cues was detected. Our study suggests that H. axyridis associated cues could influence the foraging behavior and activity of A. gifuensis under laboratory conditions. On the other hand, the foraging efficiency of A. gifuensis was not significantly affected by H. axyridis walking tracks in a single plant system. Aphidius gifuensis might exhibit flexible behavioral responses to predator associated cues. The risk of intraguild interactions and the possibility of mitigating such risks for parasitoids are important components for ultimately determining the compatibility of biological control agents. 相似文献
7.
In 1849, Isaac Lea named Sauropus primaevus for footprints from Mount Carbon, Pennsylvania, USA, then the oldest fossil vertebrate footprints reported. In 1902, O. P. Hay constructed a new ichnogenus Palaeosauropus for this ichnospecies. Palaeosauropus has been one of the most frequently reported Mississippian footprint ichnogenera in North America and remains a valid ichnotaxon. The holotype of Palaeosauropus (= “Sauropus”) primaevus (referred to as P. primaevus), consisting of a single manus/pes pair, is described and illustrated in Lea (1853) and is housed at the Academy of Natural Sciences in Philadelphia, Pennsylvania (ANS9752). Lea's large specimen of P. primaevus (approximately 86 cm by 53 cm), that included a trackway of six manus/pes pairs, described and illustrated in 1853 and 1855, was a combination of ANS9752 and a second specimen represented by a plaster cast housed at the National Museum of Natural History (USNM487148). Historical documents and examination of the Mauch Chunk Formation at Mount Carbon, Pennsylvania, enabled the identification of Lea's tracksite, originally reported to be a few hundred feet (about 75 m) from the former Mount Carbon Hotel. Our forensic evidence indicates the type locality for P. primaevus is approximately 90 m south from the southwest corner of Centre and Main Streets in Mount Carbon, Pennsylvania, with geographical coordinates of N 40° 40' 25.7”, W 76° 11' 14.9”. The type locality is within the middle member of the Mauch Chunk Formation, a fluvial sequence of late Mississippian (Visean) Age. 相似文献
8.
When a fossil vanishes to a private collection, it must be considered lost to science because, frequently, it is no longer available for study. Fortunately some fossils occasionally are regained. We had the opportunity to recoup an interesting footprint-bearing slab that was part of a private collection in Italy. The specimen, found in 1992 near Seligman, Arizona (USA) was described, before disappearing, as one of the best fossil examples of vertebrate (Chelichnus [Laoporus])-on-invertebrate (Octopodichnus) predation. After a careful re-examination of the slab, the primary conclusions of the former describers are demonstrably groundless. The reanalysis of the tracks, as well as peculiar sedimentary structures associated with the tracks, allowed obtaining new information about the depositional environment and the complex interactions between the type of substrate and trackmaker behavior. The re-examination of the specimen also revealed interesting aspects about trackmaker biomechanics. 相似文献
9.
We describe a new specimen of a supposed Paleozoic tetrapod body impression from the Lower Permian S?upiec Formation in the Intra-Sudetic Basin, Poland. The size, integument morphology of belly and part of tail imprints, and the morphology of a well-preserved pes track diagnose the specimen and readily distinguish it from other described specimens of body impressions of Paleozoic tetrapods. The eupelycosaur identity of this new specimen is based on the identification of the footprint Dimetropus leisnerianus (Geinitz, 1863), which is connected with the inferred body imprint. The morphology of integument impressions indicates the presence of the various-sized square or rectangular-shaped scales on the bottom part of the belly and tail of this eupelycosaurid trackmaker. 相似文献
10.
Trackways described as Batrachopus (Batrachopodidae Lull, 1904) from the Lower Jurassic of Europe are rare and in some cases different from the type trackways from North America. Differences may be in part attributable to preservation, but current evidence suggests that there is inherent variability in Batrachopodidae morphotypes, beyond that attributable to differential preservation. Type Batrachopus is a stout-toed form, with minimal digit divarication (i.e., a long foot), whereas Antipus describes slender-toed forms with a wider foot and wider digit divarications. Antipus is also similar to Crocodylopodus (ichnofamily Crocodylopodidae: Fuentes Vidarte and Meijide Calvo, 1999) from near the Jurassic-Cretaceous boundary in Spain. Crocodylopodus has a relatively large manus, and a less outwardly rotated trackway, but is not sufficiently different from Batrachopodidae to warrant its own ichnofamily. Manus-pes size (area) ratios (heteropody) may also be important in differentiating different crocodylomorph ichnotaxa, as is the case with other archosaurian ichnotaxa. However, heteropody may change with size, and be less pronounced in large individuals. Manus and pes rotation patterns, and trackway width are variable and may be of use for differentiation of ichnotaxa but may also be a function of speed. 相似文献