全文获取类型
收费全文 | 1577篇 |
免费 | 147篇 |
国内免费 | 49篇 |
出版年
2024年 | 5篇 |
2023年 | 30篇 |
2022年 | 37篇 |
2021年 | 69篇 |
2020年 | 69篇 |
2019年 | 86篇 |
2018年 | 73篇 |
2017年 | 66篇 |
2016年 | 55篇 |
2015年 | 61篇 |
2014年 | 93篇 |
2013年 | 176篇 |
2012年 | 53篇 |
2011年 | 57篇 |
2010年 | 50篇 |
2009年 | 83篇 |
2008年 | 77篇 |
2007年 | 54篇 |
2006年 | 57篇 |
2005年 | 39篇 |
2004年 | 46篇 |
2003年 | 46篇 |
2002年 | 42篇 |
2001年 | 25篇 |
2000年 | 20篇 |
1999年 | 37篇 |
1998年 | 26篇 |
1997年 | 33篇 |
1996年 | 23篇 |
1995年 | 16篇 |
1994年 | 13篇 |
1993年 | 12篇 |
1992年 | 15篇 |
1991年 | 24篇 |
1990年 | 17篇 |
1989年 | 10篇 |
1988年 | 8篇 |
1987年 | 4篇 |
1986年 | 8篇 |
1985年 | 12篇 |
1984年 | 11篇 |
1983年 | 2篇 |
1982年 | 8篇 |
1981年 | 8篇 |
1980年 | 5篇 |
1979年 | 4篇 |
1978年 | 2篇 |
1976年 | 2篇 |
1973年 | 1篇 |
1972年 | 1篇 |
排序方式: 共有1773条查询结果,搜索用时 31 毫秒
1.
V. G. Borkhvardt 《Russian Journal of Developmental Biology》2000,31(3):154-161
The development of the fin and limb buds involves a balance of centrifugal (active) and centripetal (passive) mechanical forces,
the first of which acts to move the walls of these structures away from each other and the second of which holds them together.
When the volume of the mesodermal core increases, the generated force meets with the resistance of the basal membrane, and
as a result, the limb bud has a tendency to acquire a cylindrical shape. Collagen fibers, individual mesenchymal cells, and
their groups hold together the dorsal and the ventral wall of the limb bud, prevent the movement of these walls away from
each other, and in this way direct bud growth along the proximodistal and the anteroposterior axes. The balance of the forces
which stretch the ectodermal layer and those which constrain it has also been observed in the development of other body parts. 相似文献
2.
E. G. Korvin-Pavlovskaya I. V. Neklyudova L. V. Beloussov 《Russian Journal of Developmental Biology》2006,37(2):77-84
Circumferential and radial components of the yolk cell surface movements were measured in the loach embryos at the late blastula stage within 40–50 min after puncture or indentation by an obliquely directed glass rod. The yolk cell surface was preliminarily marked by coal particles. It was shown that even closely located regions of the surface differed markedly in the rate and direction of their movements. In the vicinity of puncture, the yolk cell surface at first contracted in both circumferential and radial directions and then widened, but did not reach the initial values. In more remote areas, this surface continued to contract in the circumferential direction, but was extended in the radial direction. The degree of its contraction along different radii was unequal. The reaction to oblique indentation was anisotropic: the closest area of the yolk cell surface, located along the direction of indentation, contracted in both circumferential and radial directions and formed a fold “leaking” onto the rod, while the opposite area contracted in the circumferential direction, but extended in the radial direction. A conclusion was drawn that the yolk cell surface is a multivariant mechanosensitive system. Its active responses to mechanical influences obey the same patterns as multicellular embryonic tissues. 相似文献
3.
The critical weed-free period in organically-grown winter wheat 总被引:1,自引:0,他引:1
J P WELSH H A J BULSON C E STOPES R J FROUD-WILLIAMS A J MURDOCH 《The Annals of applied biology》1999,134(3):315-320
Two experiments were conducted in central southern England between September 1994 and August 1996 to identify the critical weed-free period in organically grown winter wheat (Triticum aestivum, cv. Mercia). In competition with a mixed weed infestation of predominately Alopecurus myosuroides and Tripleurospermum inodorum it was found that wheat yield decreased as the duration of the weed-infested period increased and that the crop needed to be kept free of weeds from sowing in order to completely avoid any yield loss. Also, weeds emerging in the wheat crop (predominately T. inodorum) during the growing season had a significant and detrimental effect on yield. The existence of the critical period, therefore, depends on the imposition of an acceptable yield loss. If a 5% yield loss gives a marginal benefit compared with the cost of weed control, the critical period will begin at 506°C days after sowing (November) and end at 1023°C days after sowing (February). This information could be used by farmers to target mechanical weeding operations to control weeds at a time that will have maximum benefit to the crop. 相似文献
4.
The cell surface is a mechanobiological unit that encompasses the plasma membrane, its interacting proteins, and the complex underlying cytoskeleton. Recently, attention has been directed to the mechanics of the plasma membrane, and in particular membrane tension, which has been linked to diverse cellular processes such as cell migration and membrane trafficking. However, how tension across the plasma membrane is regulated and propagated is still not completely understood. Here, we review recent efforts to study the interplay between membrane tension and the cytoskeletal machinery and how they control cell form and function. We focus on factors that have been proposed to affect the propagation of membrane tension and as such could determine whether it can act as a global or local regulator of cell behavior. Finally, we discuss the limitations of the available tool kit as new approaches that reveal its dynamics in cells are needed to decipher how membrane tension regulates diverse cellular processes. 相似文献
5.
Olga A. Grishina Irina V. Kirillova Olga E. Glukhova 《Computer methods in biomechanics and biomedical engineering》2016,19(3):297-305
The biomechanical model of human coronary arteries was modified for improving the quality of diagnosis and surgical treatment for coronary heart disease. The problem of hemodynamics in the left coronary artery with multivessel bed disease – 45% stenosis of the anterior descending branch and 75% stenosis of the circumflex branch – was particularly considered. Numerical simulation of the coronary arterial bypass of the main trunk was carried out to estimate the functional condition of the coronary arteries after restoring myocardial blood supply by surgery. 相似文献
6.
The function of the epidermis in auxinmediated elongation growth of maize (Zea mays L.) coleoptile segments was investigated. The following results were obtained: i) In the intact organ, there is a strong tissue tension produced by the expanding force of the inner tissues which is balanced by the contracting force of the outer epidermal wall. The compression imposed by the stretched outer epidermal wall upon the inner tissues gives rise to a wall-pressure difference which can be transformed into a water-potential difference between inner tissues and external medium (water) by removal of the outer epidermal wall. ii) Peeled segments fail to respond to auxin with normal growth. The plastic extensibility of the inner-tissue cell walls (measured with a constant-load extensiometer using living segments) is not influenced by auxin (or abscisic acid) in peeled or nonpeeled segments. It is concluded that auxin induces (and abscisic acid inhibits) elongation of the intact segment by increasing (decreasing) the extensibility specifically in the outer epidermal wall. In addition, tissue tension (and therewith the pressure acting on the outer epidermal wall) is maintained at a constant level over several hours of auxin-mediated growth, indicating that the inner cells also contribute actively to organ elongation. However, this contribution does not involve an increase of cell-wall extensibility, but a continuous shifting of the potential extension threshold (i.e., the length to which the inner tissues would extend by water uptake after peeling) ahead of the actual segment length. Thus, steady growth involves the coordinated action of wall loosening in the epidermis and regeneration of tissue tension by the inner tissues. iii) Electron micrographs show the accumulation of striking osmiophilic material (particles of approx. 0.3 m diameter) specifically at the plasma membrane/cell-wall interface of the outer epidermal wall of auxin-treated segments. iv) Peeled segments fail to respond to auxin with proton excretion. This is in contrast to fusicoccin-induced proton excretion and growth which can also be readily demonstrated in the absence of the epidermis. However, peeled and nonpeeled segments show the same sensitivity to protons with regard to the induction of acid-mediated in-vivo elongation and cell-wall extensibility. The observed threshold at pH 4.5–5.0 is too low to be compatible with a second messenger function of protons also in the growth response of the inner tissues. Organ growth is described in terms of a physical model which takes into account tissue tension and extensibility of the outer epidermal wall as the decisive growth parameters. This model states that the wall pressure increment, produced by tissue tension in the outer epidermal wall, rather than the pressure acting on the inner-tissue walls, is the driving force of growth.Abbreviations and symbols
E
el, E
pl
elastic and plastic in-vitro cell-wall extensibility, respectively
-
E
tot
E
el+E
pl
- FC
fusicoccin
- IAA
indole-3-acetic acid
- IT
inner tissue
- ITW
inner-tissue walls
- OEW
outer epidermal wall
-
osmotic pressure
-
P
wall pressure
-
water potential 相似文献
7.
The effect of excitation on the rate of respiration in the liverwort Conocephalum conicum 总被引:4,自引:0,他引:4
Simultaneous measurements were taken of the electrical activity and the rate of respiration of thalli of Conocephalum conicum L. stimulated electrically and mechanically (by cutting). The measurements of the rate of respiration employed a modified Warburg apparatus for O2 consumption and an infra-red gas analyzer with computer recording and data processing for CO2 evolution. The action potential, produced by either a cut (a damaging stimulus) or an electrical stimulus (a non-damaging stimulus), caused a transient rise in the rate of respiration. The course of changes in the rate of respiration depends on the character of the excitation and the area of the thallus covered by it. If stimulation does not produce excitation, the increase in the rate of respiration does not take place, regardless of the magnitude and type of the stimulus applied. 相似文献
8.
Genetical and ecological differentiation across a hybrid zone 总被引:1,自引:0,他引:1
ABSTRACT.
- 1 There is a narrow hybrid zone between two chromosomal races of the grasshopper Podisma pedestris (L.) in the French Alps.
- 2 There are no gross differences in habitat across the zone, and a multivariate analysis of the vegetation composition detected no significant transition either.
- 3 The hatch and development rates of Podisma in hybrid populations were lower than those found in matched habitat types outside the zone.
- 4 The simplest explanation for the persistence of the parapatric distribution of the two races is that hybrid disadvantage is maintaining a tension zone between them.
9.
Penetrometer resistance,root penetration resistance and root elongation rate in two sandy loam soils
Root penetration resistance and elongation of maize seedling roots were measured directly in undisturbed cores of two sandy
loam soils. Root elongation rate was negatively correlated with root penetration resistance, and was reduced to about 50 to
60% of that of unimpeded controls by a resistance of between 0.26 and 0.47 MPa. Resistance to a 30° semiangle, 1 mm diameter
penetrometer was between about 4.5 and 7.5 times greater than the measured root penetration resistance. However, resistance
to a 5° semiangle, 1 mm diameter probe was approximately the same as the resistnace to root penetration after subtracting
the frictional component of resistance. The diameter of roots grown in the undisturbed cores was greater than that of roots
grown in loose soil, probably as a direct result of the larger mechanical impedance in the cores. 相似文献
10.
冷冻液温和季节对鼠尾过冷点的影响 总被引:3,自引:0,他引:3
为研究动物对寒冷的适应性,将鼠尾置于冷液浸冻,发现在一定条件下鼠尾组织可发生过冷现象。实验表明,鼠尾组织的过冷点和冷冻液温有关,同一季节冷冻液温越低过冷点越高;而不同季节相同冷冻条件下,冬季鼠尾组织的过冷点明显低于春季。 动物肢体组织的过冷特性是动物的抗寒冷特性,它和组织自身的物理化学性质有关。理沦证明,过冷度(△T)和表面张力(O)、摩尔质量(M),冰点(Ti)、密度(p)、摩尔凝固热(△H)及冰胚临界半径(rk)有关,其关系式为△T=26MTi/p△Hrk. 相似文献