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1.
Kleinow  W.  Röhrig  A. 《Hydrobiologia》1995,(1):171-174
A method is described by which the integument of Brachionus plicatilis, together with its intracellular lamina, is quickly dissolved before other parts or tissues of the animal are destroyed. After removing the integument several parts of the body can be separated and fractionated in a more or less intact state by centrifugation in a Percoll gradient. The measurement of enzyme activities has indicated that this procedure might provide a way of localizing enzymes within the rotifer body.  相似文献   
2.
Ultrastructural and protargol studies reveal that the trophont of Lagenophrys callinectes, though highly specialized, generally conforms to the basic peritrich structural pattern. Features described for L. callinectes trophonts which are unique for the genus are the fine structure and arrangement of the lorica and lips, the attachment organelle of the peristomial cytoplasm, its attachment to the loricastome walls, and the arrangement of the aboral kinetosomes of the trophont. Lack of a distinct scopularized region, and of a ventral lorica wall also characterize L. callinectes trophonts. The 4-row terminal peniculus, as revealed by protargol staining, differs from the 6-row terminal peniculus of L. nassa suggesting that the patterns of infundibular structure, as revealed by protargol, should be useful in future taxonomic studies of Lagenophrys species.  相似文献   
3.
ABSTRACT. Cell division in Acanthoeca spectabilis produces a "naked" motile daughter cell (juvenile) that settles onto a surface and deposits siliceous costal strips that are stored extracellularly in bundles. When complete, the bundles of strips are assembled in a single continuous movement to form a basket-like lorica. Assembly can be divided into four overlapping stages. Stage 1 entails the left-handed rotation of strips at the anterior end while the posterior end remains stationary. Stage 2 includes the posterior protrusion of the cell to form a stalk. Stage 3 involves the anterior extension of the spines, and Stage 4 the dilation of the lorica chamber and deposition of the organic investment. Scanning electron microscopic images reveal a one-to-one association between the moving bundles of strips and the anterior ring of lorica-assembling tentacles. Treatment with microtubule inhibitors produces "dwarf" cells that lack stalks, have their spines extended, and possess collars but lack flagella. Treatment with microfilament (actin) inhibitors prevents extension of the anterior spines. These experiments demonstrate that posterior cell extension is primarily mediated by microtubules whereas extension of the spines is controlled by the actin cytoskeleton. The processes of cytoskeletal rotation and extracellular costal strip movement are compared, respectively, with rotation of nuclei in animal embryos and movement of mammalian cells over surfaces.  相似文献   
4.
A new tintinnid ciliate, Tintinnopis estuariensis Zhang, Feng Yu, sp. nov. was found in the estuary of Yangtze River, China. It was most abundant in September. Its arenaceous lorica is cylindro-conical in shape,(120–180) μm×(50–75) μm in size, agglutinated with mineral particles, and open at both ends. Two characters distinguish it from others: the unique lateral flattened posterior portion with a vertical aboral opening and wider oral diameter. It occurred in water with surface temperature of 14–27°C and surface salinity of 3.9‰–29.8‰.  相似文献   
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Growth, turnover of silica and lorica morphology of Stephanoeca diplocostata Ellis have been investigated in batch cultures at 20 °C. Mean cell doubling times for separate experiments ranged from 9.1–13.8 h. During exponential growth, uptake of reactive silicate progressed steadily and throughout this phase the average amount of biogenic silicon per cell was 2.1 pg. Once growth declined, net dissolution of silica from loricae became apparent and progressed steadily throughout the stationary and death phases. The minor difference in solubility between loricae of living cells and costal strips cleaned with acid indicates that even if an organic component is associated with the silica of costal strips it does not inhibit silica dissolution. The first effects of silica dissolution on cells as revealed by electron microscopy are limited to corrosion on the surfaces and the hollowing out of the centres of costal strips but ultimately a consistent pattern of lorica disintegration occurs.  相似文献   
8.
Choanoflagellates are unicellular filter-feeding protozoa distributed universally in aquatic habitats. Cells are ovoid in shape with a single anterior flagellum encircled by a funnel-shaped collar of microvilli. Movement of the flagellum creates water currents from which food particles are entrapped on the outer surface of the collar and ingested by pseudopodia. One group of marine choanoflagellates has evolved an elaborate basket-like exoskeleton, the lorica, comprising two layers of siliceous costae made up of costal strips. A computer graphic model has been developed for generating three-dimensional images of choanoflagellate loricae based on a universal set of 'rules' derived from electron microscopical observations. This model has proved seminal in understanding how complex costal patterns can be assembled in a single continuous movement. The lorica, which provides a rigid framework around the cell, is multifunctional. It resists the locomotory forces generated by flagellar movement, directs and enhances water flow over the collar and, for planktonic species, contributes towards maintaining cells in suspension. Since the functional morphology of choanoflagellate cells is so effective and has been highly conserved within the group, the ecological and evolutionary radiation of choanoflagellates is almost entirely dependent on the ability of the external coverings, particularly the lorica, to diversify.  相似文献   
9.
ABSTRACT. A membranous closing apparatus shuts the lorica opening in disturbed tintinnids of six genera belonging to four families. The homology of the apparatuses is investigated, using data from the literature and Mediterranean tintinnids studied in vivo and by scanning electron microscopy. Morphological and functional similarities indicate that the foldable closing apparatus is not only a synapomorphy of the genera Codonella (Codonellidae) and Dictyocysta (Dictyocystidae), as suggested 80 years ago, but also of Codonaria (Codonellidae) and Codonellopsis (Codonellopsidae). In Codonaria, Codonella, and Dictyocysta, the apparatuses merge posteriorly into membranous lorica sacs, which probably represent homologous structures. The diagnoses of these genera are improved according to the new findings. The close relationship of Codonella, Codonellopsis, and Dictyocysta is also inferred from small subunit rRNA phylogenies and the ultrastructure of the capsules. It contradicts the current lorica‐based classification of the tintinnids. The assumption that the diaphragm‐like apparatus in the genera Salpingacantha and Salpingella is not homologous to the foldable ones in the genera mentioned above is supported by molecular and cytological features.  相似文献   
10.
Synchroma grande (Synchromophyceae, Heterokontophyta) is a marine amoeboid alga, which was isolated from a benthic habitat. This species has sessile cell stages (amoeboid cells with lorica and cysts) and non‐sessile cell stages (migrating and floating amoebae) during its life cycle. The different cell types and their transitions within the life cycle are described, as are their putative functions. Cell proliferation was observed only in cells attached to the substrate but not in free‐floating or migrating cells. We also characterised the phagotrophy of the meroplasmodium in comparison to other amoeboid algae and the formation of the lorica. The functional adaptations of S. grande during its life cycle were compared to the cell stages of other amoeboid algae of the red and green chloroplast lineages. S. grande was found to be highly adapted to the benthic habitat. One sexual and two asexual reproductive strategies (haplo‐diploid life cycle) support the ability of this species to achieve rapid diversification and high adaptivity in its natural habitat.  相似文献   
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