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MOGENS MADSEN GUNNER HØI SØRENSEN SØREN ACHIM NIELSEN † 《Medical and veterinary entomology》1991,5(4):421-429
The summer mastitis pathogens Actinomyces pyogenes, Peptococcus indolicus, Bacteroides melaninogenicus ss. levii, Fusobacterium necrophorum and Streptococcus dysgalactiae were isolated from the polyphagous symbovine dipterans Hydrotaea irritans (Fallén) and Morellia sp. caught around dairy heifers on pasture, but not from the haematophagous species Haematobia irritans (L.), Haematobosca stimulans (Meigen), Culicoides sp. and Simulium sp. Secretions from clinical cases of summer mastitis proved to be sources of summer mastitis bacteria for more than 3 weeks despite antibiotic treatment and teat amputation. Taking into account the seasonal activity pattern of Hydrotaea irritans and its topographical distribution on grazing cattle, it appears evident that this fly may play a central role in the establishment and maintenance of the bacterial contamination with summer mastitis pathogens on the teats of healthy cattle. In the present study the survival of A.pyogenes and P.indolicus for 7 days in experimentally infected Hydrotaea irritans, as demonstrated by the recovery of these microorganisms from agar plates exposed to live infected flies, is described. However, experimental transmission of summer mastitis from sick to healthy heifers by Hydrotaea irritans proved unsuccessful. 相似文献
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Kyaw Kyaw Moe Takahisa Yano Kazuhiro Misumi Chikara Kubota Kazumi Nibe Wataru Yamazaki Michio Muguruma Naoaki Misawa 《Microbiology and immunology》2010,54(6):338-346
Bovine digital epidermitis involves different pathologies, including PDD, interdigital dermatitis, and foot rot. Bacteriological and molecular biological studies suggest that these are multimicrobial infections. During our study on the isolation of treponemes from biopsies of PDD, colonies producing black pigment were isolated frequently from the primary cultures, suggesting that Porphyromonas species were present. Moreover, 16S rRNA genes of Fusobacterium necrophorum and Porphyromonas levii‐like species were detected in the lesions. We therefore determined whether an immunological response could be elicited by a P. levii‐like organism isolated from a PDD lesion, as well as two subspecies of F. necrophorum in the sera from cows with and without PDD. A total of 151 serum samples were collected from 85 cows with PDD lesions and 33 cows without lesions on 12 PDD‐positive farms and from 33 cows on two PDD‐free farms. ELISA data showed that IgG antibody levels against antigens of P. levii‐like species and F. necrophorum subsp. necrophorum were significantly higher in cows on PDD‐positive farms than in cows on PDD‐free farms, regardless of the presence of PDD lesions in the cows on the PDD‐positive farms. However, F. necrophorum subsp. funduliforme was present at low levels in both groups. The ELISA results were confirmed by western blot analysis. Furthermore, antigens of these bacteria were detected in PDD‐biopsy sections examined by immunohistochemical staining. F. necrophorum subsp. necrophorum and P. levii‐like species may be involved in the pathogenesis of PDD. 相似文献
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