全文获取类型
收费全文 | 510篇 |
免费 | 74篇 |
国内免费 | 22篇 |
出版年
2024年 | 5篇 |
2023年 | 3篇 |
2022年 | 5篇 |
2021年 | 5篇 |
2020年 | 19篇 |
2019年 | 16篇 |
2018年 | 16篇 |
2017年 | 28篇 |
2016年 | 24篇 |
2015年 | 15篇 |
2014年 | 23篇 |
2013年 | 40篇 |
2012年 | 10篇 |
2011年 | 31篇 |
2010年 | 11篇 |
2009年 | 17篇 |
2008年 | 37篇 |
2007年 | 29篇 |
2006年 | 24篇 |
2005年 | 21篇 |
2004年 | 18篇 |
2003年 | 13篇 |
2002年 | 19篇 |
2001年 | 10篇 |
2000年 | 11篇 |
1999年 | 17篇 |
1998年 | 8篇 |
1997年 | 6篇 |
1996年 | 11篇 |
1995年 | 7篇 |
1994年 | 14篇 |
1993年 | 12篇 |
1992年 | 8篇 |
1991年 | 9篇 |
1990年 | 5篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1987年 | 4篇 |
1986年 | 3篇 |
1985年 | 7篇 |
1984年 | 7篇 |
1983年 | 8篇 |
1982年 | 6篇 |
1981年 | 4篇 |
1980年 | 8篇 |
1979年 | 3篇 |
1978年 | 1篇 |
1976年 | 2篇 |
1973年 | 2篇 |
1971年 | 1篇 |
排序方式: 共有606条查询结果,搜索用时 15 毫秒
1.
(Ni-Sn-M_xO_y)/Pb改性复合电极电解合成L-半胱氨酸的反应 总被引:1,自引:0,他引:1
在(Ni-Sn)/Pb合金修饰电极中添加某些金属氧化物进行改性,用于电解合成L-半胱氨酸反应。结果证明,添加WO3后电极反应活性大大加强,反应同期转化率有较大提高,选择性也有所提高,添加稀土金属氧化物能有效降低反应阴极过电位,使反应选择性有所提高,但是电极稳定性还有待改善。 相似文献
2.
The binding of lipophilic ions to the membrane of envelope vesicles from Halobacterium halobium was examined in the absence and presence of membrane potential. The lipophilic ions used constitute a homologous series of (Phe)3-P+-(CH2)n-CH3 (n = 0–4) and tetraphenylphosphonium (TPP+). In the absence of membrane potential, the amounts of binding were proportional to the probe concentration in the medium when the concentration is dilute. Upon illumination, interior negative membrane potential is generated which induces the uptake of phosphonium cation probe. 2 μM were employed as the initial probe concentration. The real membrane potential was evaluated by means of extrapolation to the state of no binding: The values of
for various probes are plotted against the binding coefficient. Here, Ciapp is the apparent intra-vesicular concentration of the probes which is calculated without consideration of bound probes. The ordinate intercept of the plot gives the true concentration ratio, and from this the membrane potential is evaluated. The membrane potential-dependent binding was analysed with a model: the membrane is split into two halves, outer and inner half, and the amounts of bound probes in each region are governed by the concentration in the contiguous solution. We obtained a formula which describes amounts of binding as a function of the membrane potential. 相似文献
3.
T J Holmes J L Vennerstrom V John 《Biochemical and biophysical research communications》1984,123(1):156-162
Irreversible inhibition of soybean lipoxygenase-1 (SL-1) was accomplished via a controlled potential oxidative electrolysis of 1,5-dihydroxynaphthalene (1,5-DHN) at +0.8 V vs SCE. The inactivation of SL-1 with this known inhibitor was greatly enhanced under these electrolytic conditions to which the enzyme itself was stable. Electrolyses were run at 0 degree C in a 0.05 M phosphate buffer, pH 7.0, using graphite cloth electrodes. The rate of inactivation was observed to be limited by and dependent on the anodic oxidation of 1,5-DHN. The non-oxidizable (at this potential) inhibitor indomethacin was shown to protect the enzyme from irreversible inactivation, however, an external nucleophile (2-mercaptoethanol) had little effect. These initial studies support the capability of such electrochemical methods for the site-specific covalent modification (affinity labelling) of lipoxygenase, and perhaps other enzymes. 相似文献
4.
The preparation and use of a specific cDNA probe for quantitating mRNA by solution hybridization is described. Cloned DNA sequences are nick translated, denatured, hybridized to single-stranded M13 clones containing message strand (mDNA) sequences, and separated chromatographically on Bio-Gel A50 under first native and then denaturing conditions to yield a single-stranded cDNA probe. The details of a solution hybridization assay in which the single-stranded cDNA is used to quantitate mRNA in total nucleic acid samples are described. As little as 0.5 pg of mRNA can easily be detected within a day of sample isolation. Thus, the assay is both rapid and sensitive and can be used to measure RNAs complementary to any cloned DNA sequence. It is ideally suited to situations when accurate quantitation of multiple samples is anticipated. 相似文献
5.
Frank Thévenod Martine Dehlinger-Kremer Thomas P. Kemmer Anna-Luise Christian Barry V. L. Potter Irene Schulz 《The Journal of membrane biology》1989,109(2):173-186
Summary We have measured Ca2+ uptake and Ca2+ release in isolated permeabilized pancreatic acinar cells and in isolated membrane vesicles of endoplasmic reticulum prepared from these cells. Ca2+ uptake into cells was monitored with a Ca2+ electrode, whereas Ca2+ uptake into membrane vesicles was measured with45Ca2+. Using inhibitors of known action, such as the H+ ATPase inhibitors NBD-Cl and NEM, the Ca2+ ATPase inhibitor vanadate as well as the second messenger inositol 1,4,5-trisphosphate (IP3) and its analog inositol 1,4,5-trisphosphorothioate (IPS3), we could functionally differentiate two non-mitochondrial Ca2+ pools. Ca2+ uptake into the IP3-sensitive Ca2+ pool (IsCaP) occurs by a MgATP-dependent Ca2+ uptake mechanism that exchanges Ca2+ for H+ ions. In the absence of ATP Ca2+ uptake can occur to some extent at the expense of an H+ gradient that is established by a vacuolar-type MgATP-dependent H+ pump present in the same organelle. The other Ca2+ pool takes up Ca2+ by a vanadate-sensitive Ca2+ ATPase and is insensitive to IP3 (IisCaP). The IsCaP is filled at higher Ca2+ concentrations (10–6 mol/liter) which may occur during stimulation. The low steady-state [Ca2+] of 10–7 mol/liter is adjusted by the IisCaP.It is speculated that both Ca2+ pools can communicate with each other, the possible mechanism of which, however, is at present unknown. 相似文献
6.
Alexander A. Bulychev Tijmen van Voorthuysen Wim J. Vredenberg 《Physiologia plantarum》1996,98(2):605-611
Electrochemical data obtained with TMPD+ -sensitive electrodes indicate that ammonium-uncoupled chloroplasts retain TMPD (N,N,N',N'-tetramethyl- p -phenylenediamine) mainly in the reduced form during illumination, whereas uncoupled DCMU-treated chloroplasts accumulate TMPD in the oxidized form (TMPD+ ). This observation indicates that the reduced plastoquinol is the preferred electron donor for photosystem I (PSI) and TMPD can only compete efficiently when plastoquinone reduction is blocked. After adding DCMU the formation of a transmembrane gradient for TMPD+ is reflected by a slow-down of the electrogenic electron transport and by the emerging of the overshoot of the membrane current in the light-off response. A light-dependent increase in photoelectric current generated by chloroplasts in the presence of NH4 Cl and TMPD is observed and considered to be caused by a reversible release of current limitation in the interfacial conductance barriers in the lumen. 相似文献
7.
C. L. Devlin P. J. S. Smith 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1996,166(4):270-277
To determine possible sources of Ca2+ during excitation-contraction coupling in smooth muscle, a vibrating Ca2+-selective electrode was used to measure Ca2+ flux during the process of contraction. The smooth muscle model was the longitudinal muscle of the body wall of a sea cucumberSclerodactyla briareus. Because acetylcholine caused slow contractions of the muscle that were inhibited by Ca2+ channel blockers diltiazem and verapamil in earlier mechanical studies, we chose a vibrating Ca2+-selective electrode as our method to test the hypothesis that acetylcholine may be stimulating Ca2+ influx across the sarcolemma, providing a Ca2+ source during excitation-contraction coupling. Acetylcholine treatment stimulated a net Ca2+ efflux that was both dose and time dependent. We then tested two L-type Ca2+ channel blockers, diltiazem and verapamil, and two non-specific Ca2+ blockers, cobalt (Co2+) and lanthanum (La3+) on acetylcholine-induced Ca2+ flux. All four Ca2+ blockers tested potently inhibited Ca2+ efflux induced by physiological doses of acetylcholine. We propose that the acetylcholine-induced Ca2+ efflux was the result of, first, Ca2+ influx through voltage-sensitive L-type Ca2+ channels, then the rapid extrusion of Ca2+ by an outwardly directed carrier such as the Na–Ca exchanger as suggested by Li+ substitution experiments. The vibrating Ca2+ electrode has provided new insights on the active and complex role the sarcolemma plays in Ca2+ homeostasis and regulating Ca2+ redistribution during excitation-contraction coupling.Abbreviations
ACh
acetylcholine
-
E-C coupling
excitation-contraction coupling
-
LMBW
longitudinal muscle of the body wall 相似文献
8.
系统地研究了细胞色素c在多种氨基酸和多肽修饰电极上的电化学反应。并对影响加速细胞色素c电化学反应的因素进行了讨论。 相似文献
9.
Presynaptic modulation by eicosanoids in cortical synaptosomes 总被引:1,自引:0,他引:1
In continuing experiments to determine the ionic basis of inhibitory presynaptic modulation, rat cortical synaptosomes were employed and receptor-activated K+ efflux was determined with a K+ sensitive electrode. When synaptosomes were sub-optimally depolarized by veratridine, the addition of agents that activated purinergic, 2, muscarinic and opioid receptors all promoted K+ efflux. With 2-chloroadenosine as a model inhibitory presynaptic modulator, the increased K+ efflux evoked by this agent was blocked by the cyclooxygenase inhibitor indomethacin suggesting that arachidonic acid or its metabolites was an intermediary in opening the channel. When arachidonic acid and PGE2 were tested, both promoted K+ efflux that was inhibited by dendrotoxin and mast cell degranulating peptide, two agents that are known to inhibit a delayed rectifier K+ current. Our results suggest that via eicosanoid second messengers, inhibitory presynaptic modulators open a sub-class of K channels that hyperpolarize nerve terminals, therefore less Ca2+ would enter per nerve impulse and thus the evoked release of neurotransmitters would be decreased.Abbreviations DTX
dendrotoxin
- MCDP
mast cell degranulating peptide
- NHGA
norhydroguairetic acid
- PGE2
prostaglandin E2 相似文献
10.
几种环境因素对玉米叶切块光诱导的影响 总被引:1,自引:1,他引:0
利用液相氧电极研究了暗处理、光强、CO2水平、缺钾和干旱对玉米叶切块光诱导的影响。发现长时间的暗处理、高光强、低CO2浓度、缺钾和干旱均使光合诱导期拉长。影响光合诱导期的外界逆境如低CO2浓度、缺钾和干旱也使玉米叶片的净光合速率下降,并对这些结果产生的原因作了分析。 相似文献