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排序方式: 共有99条查询结果,搜索用时 15 毫秒
1.
Jean-Vincent Escalant Claude Teisson François Cote 《In vitro cellular & developmental biology. Plant》1994,30(4):181-186
Summary Somatic embryogenesis and plant regeneration of banana and plantain cultivars (Musa spp.) were obtained by culturing young male flowers. Multiplication and maintenance of embryogenic cultures were achieved
by culturing somatic embryos in a temporary immersion system (SIT). A multiplication rate of 40 allowed us to obtain more
than 6000 somatic embryos after 6 mo. of subculture. Plant recovery frequencies were 60 to 70%. This method was expanded to
different banana and plantain genomic groups. 相似文献
2.
Gregory Polyzois Artemis Niarchou Polyxeni Ntala Antonis Pantopoulos Mary Frangou 《Gerodontology》2013,30(2):150-156
doi: 10.1111/j.1741‐2358.2012.00657.x The effect of immersion cleansers on gloss, colour and sorption of acetal denture base material Objective: To study the effect of peroxide and hypochlorite cleansers on gloss, colour and sorption of acetal denture resins. Materials and methods: Pink acetal and thermoplastic acrylic resins were evaluated. Thirty‐five specimens 39 × 39 × 1.8 mm of each resin were prepared. Each group of specimens (n = 7) was then immersed into cleansers for 100 days. Group I immersed in tap water, Group II in Corega Extradent for 5 min, Group III in Corega Extradent for 8 h, Group IV in NitrAdine? Seniors for 15 min and Group V in NaOCl 5.25%. Gloss, colour and weight measurements were taken initially and after 100 days. Data subjected to two‐way anova and Tukey’s test at α = 0.05. Results: Acrylic resin showed reductions of glossiness from ?5 to ?15 and acetal from ?0.2 to ?6. Colour changes (ΔΕ*) ranged from 2.64 to 7.64 for acrylic and 2.77 to 26.54 for acetal resin. Sorption for acrylic ranged from 11.64 to 17.06 μg/mm3 and 9.18 to 24.79 μg/mm3 for acetal resin. The results of (ΔΕ*) and sorption showed an interaction between denture resins and cleansers. Conclusions: The gloss of acetal resin was less affected by water, peroxides and NaOCl 5.25% compared with acrylic resin. Acetal resin showed clinically acceptable (ΔΕ*) whereas acrylic resin unacceptable ones for water and peroxide solutions. The immersion of acetal resin in NaOCl 5.25% showed clinically unacceptable (ΔΕ*) and higher sorption and should be avoided or should be managed with care. 相似文献
3.
《Cryobiology》2019
Several types of cryostimulation have been recently proposed to rapidly lower skin temperature therefore gaining a possible neuro/muscular recovery after strenuous exercise or, more generally, in sports. Local cryostimulation may be a viable and relatively portable tool to obtain physiological benefits in previously-efforted muscular districts. However, cohesive and standardized cryo-exposure protocols are lacking as well as the righteous procedure to efficaciously combine duration, treatments and temperature in relation to desirable effects on muscular strength. In this randomized-controlled study, fifty young women were tested for maximum isometric handgrip strength, before and after exhausting contractions.Following the fatiguing protocol, the intervention group (cryo, n = 25, 24.7 ± 2.5 years, BMI 21.7 ± 1.8 kg/m2) underwent a 6-min local cryostimulation (−160 °C) on the extensor-flexor muscles of the dominant arm, while control-matched peers sat rested in a thermo-neutral room (22 ± 0.5 °C). Handgrip tests were repeated at baseline (T0), after cryostimulation (T1), and 15 min after T1 (T2). Throughout the protocol, the AUC of the strength performance was significantly higher in the cryo- compared to control group (P = 0.006). In particular, following fatigue and cryostimulation, the cryo group preserved higher strength at T1 with respect to controls (26.8 ± 2.8 vs 23.9 ± 2.8 kg, Bonferroni's post-hoc, P < 0.01). Likewise, ventral and dorsal temperature, recorded with a thermal camera, were lower in cryo- than control group (P < 0.0001).In conclusion, a brief session of local cryostimulation may acutely preserve maximal isometric force in young women following a fatiguing protocol. These findings may have implications in orchestrating strategies of district muscular recovery. 相似文献
4.
The polyethylene glycol (PEG) treatment of ciprofloxacin-Indion 234 complex was aimed to retard rapid ion exchange drug release
at gastric pH. Ciprofloxacin loading on Indion 234 was performed in a batch process, and the amount of K+ in Indion 234 displaced by drug with time was studied as equilibrium constant KDM. Drug-resin complex (DRC) was treated with aqueous PEG solution (0.5%–2% wt/vol) of different molecular weights (MWs) for
2 to 30 minutes. The PEG-treated ciprofloxacin-Indion 234 complex was evaluated for particle size, water absorption time,
and drug release at gastric pH. During drug loading on Indion 234, the equilibrium constant (KDM) increased rapidly up to 20 minutes with efficient drug loading. Increased time of immersion of the drug resinate in PEG
solutions significantly retained higher size particles upon dehydration. The larger DRC particles showed longer water absorption
times owing to compromised hydrating power. The untreated DRC showed insignificant drug release in deionized water; while
at gastric pH, ciprofloxacin release was complete in 90 minutes. A trend of increased residual particle size, proportionate
increase in water absorption time, and hence the retardation of release with time of immersion was evident in PEG-treated
DRC. The time of immersion of DRC in PEG-treated DRC. The time of immersion of DRC in PEG solution had predominant release
retardant effect, while the effect of molecular weight of PEG was insignificant. Thus, PEG treatment of DRC successfully retards
ciprofloxacin ion exchange release in acidic pH. 相似文献
5.
Abstract In contrast to floating on the surface of distilled water, ants were immediately submerged after being placed in the potassium oleate (PO) water solution, which led to immobilization within minutes. However, some workers survived after being immersed in 0.03% PO water solution at 25°C for up to 640 min. Elevated temperature of the PO water solution is needed to kill ants within a shorter time frame. At 50°C and 0.13% PO concentration, total mortality was achieved with 10-min immersion for all ants, including brood and adult ants. Soil has a negative effect on the effectiveness of potassium oleate; however, such negative effect can be overcome by increasing either treatment temperature or duration of the treatment. In addition to immobilizing and lethal effect, PO repels ants. PO may have the potential to be incorporated into immersion treatment for the quarantine of imported fire ants to reduce the use of synthetic contact insecticides. 相似文献
6.
The present article describes two new applications of plastic-based cell culture systems in the plant biotechnology domain.
Different types of bioreactors are used at Nestlé R&D Center-Tours for large scale culture of plants cells to produce metabolites
or recombinant proteins and for mass propagation of selected plant varieties by somatic embryogenesis. Particularly, recent
studies are directed to cut down the production costs of these two processes by developing disposable cell culture systems.
For large scale culture, two novel flexible plastic-based disposable bioreactors have been developed from 10 to 100 l working
volumes, validated with several plant species (“Wave and Undertow” and “Slug Bubble” bioreactors). Vegetative propagation
of elite plant varieties is achieved through somatic embryogenesis in liquid medium. A pilot scale process has been recently
set up for the industrial propagation of Coffea canephora (Robusta coffee). The current production capacity is 2.5–3.0 million embryos per year. The pre-germination of the embryos
was previously conducted by temporary immersion in liquid medium in 10-l glass bioreactors. An improved process has been developed
using a 10-l disposable bioreactor consisting in a bag containing a rigid plastic box (“Box-in-Bag” bioreactor), insuring,
amongst other advantages, a higher light transmittance to the biomass due to its horizontal design. 相似文献
7.
8.
9.
Susan J. Murch Chunzhao Liu Rosaura M. Romero Praveen K. Saxena 《Plant Cell, Tissue and Organ Culture》2004,78(1):63-68
Crescentia cujete L. is a widely distributed medicinal tree with a diverse range of phytochemicals used as medicinal compounds. Seedlings of
wild-harvested C. cujete were established in vitro and used as the starting material for the establishment of axenic cultures. Shoots were proliferated from nodal segments
and were maintained over a period of more than 2 years by sequential subculture on a medium containing 1.0 μmol l−1 kinetin. De novo regeneration was induced on petiole sections cultured onto a medium containing thidiazuron in combination with 2,4-dichlorophenoxyacetic
acid. Axenic cultures were also used to test the efficiency of three different cultivation systems for production of biomass
of C. cujete. Growth of plantlets in a temporary immersion bioreactor resulted in significant increases in biomass, leaf number, shoot
height and transplant efficiency. Plantlets grown in the bioreactors were acclimatized under greenhouse conditions. Together,
these experiments have established optimized parameters for propagation and growth of C. cujete plantlets in a sterile controlled environment for biochemical characterization and production of high-quality medicinal products. 相似文献
10.
H. A. M. Daanen F. J. G. Van de Linde T. T. Romet M. B. Ducharme 《European journal of applied physiology and occupational physiology》1997,76(6):538-543
The relationship between body temperature and the hunting response (intermittent supply of warm blood to cold exposed extremities)
was quantified for nine subjects by immersing one hand in 8°C water while their body was either warm, cool or comfortable.
Core and skin temperatures were manipulated by exposing the subjects to different ambient temperatures (30, 22, or 15°C),
by adjusting their clothing insulation (moderate, light, or none), and by drinking beverages at different temperatures (43,
37 and 0°C). The middle finger temperature (T
fi) response was recorded, together with ear canal (T
ear), rectal (T
re), and mean skin temperature (Tˉ
sk). The induced mean T
ear changes were −0.34 (0.08) and +0.29 (0.03)°C following consumption of the cold and hot beverage, respectively. Tˉ
sk ranged from 26.7 to 34.5°C during the tests. In the warm environment after a hot drink, the initial finger temperature (T
fi,base) was 35.3 (0.4)°C, the minimum finger temperature during immersion (T
fi,min) was 11.3 (0.5)°C, and 2.6 (0.4) hunting waves occurred in the 30-min immersion period. In the neutral condition (thermoneutral
room and beverage) T
fi,base was 32.1 (1.0)°C, T
fi,min was 9.6 (0.3)°C, and 1.6 (0.2) waves occurred. In the cold environment after a cold drink, these values were 19.3 (0.9)°C,
8.7 (0.2)°C, and 0.8 (0.2) waves, respectively. A colder body induced a decrease in the magnitude and frequency of the hunting
response. The total heat transferred from the hand to the water, as estimated by the area under the middle finger temperature
curve, was also dependent upon the induced increase or decrease in T
ear and Tˉ
sk. We conclude that the characteristics of the hunting temperature response curve of the finger are in part determined by core
temperature and Tˉ
sk. Both T
fi,min and the maximal finger temperature during immersion were higher when the core temperature was elevated; Tˉ
sk seemed to be an important determinant of the onset time of the cold-induced vasodilation response.
Accepted: 29 April 1997 相似文献