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1.
This paper discusses the properties of bacterial haem-containing and non-haem haloperoxidases, their involvement in the biosynthesis of halometabolites and their use in bioconversion. The very low peroxidase activity of bacterial non-haem haloperoxidases, their stability at high temperature and over a wide pH-range makes them particularly suited for use in the bromination of organic compounds. The chloroperoxidase from Pseudomonas pyrrocinia is the only haloperoxidase showing substrate specificity and regioselectivity. The genes of one chloro- and one bromoperoxidase could be cloned. The corresponding enzymes can now be produced in large amounts and at low costs. 相似文献
2.
Complete accounts of the natural products chemistry of Bonnemaisonia nootkana, B. asparagoides, B. hamifera and Trailliella intricata are described. In contrast to the chemistry of the closely related alga Asparagopsis, Bonnemaisonia spp. do not produce halomethanes, but instead an array of C7-C9 halogen-containing ketones, alcohols and carboxylic acids. Biomimetic syntheses of these compounds suggest they are precursors and products of in vivo Favorsky rearrangements. 相似文献
3.
The red marine algae Microcladia borealis, M. californica and M. coulteri produce several unusual halogenated monoterpenes including violacene, plocamene-B, plocamene-C, and plocamane-D. The isolation of these terpenes along with a study of their variation in each Microcladia at different locations are described. 相似文献
4.
William Fenical 《Journal of phycology》1975,11(3):245-259
The halogens—chlorine, bromine and iodine—play an important, role in the biochemical processes of marine red algae. Recent studies show that various species from at least 5 orders of the Rhodophyta possess the unique ability to synthesize organic halogen-containing compounds which are derived from seawater components. A variety of substances have been reported, with various structures from simple aliphatic halo-ketones and brominated phenols to more sophisticated mono-, sesqui- and diterpenes. While the biological functions of these compounds are not clearly understood, they appear to provide environmental advantage, probably involving predator avoidance responses and microflora antibiosis. 相似文献
5.
John J. Ross Nathan D. Tivendale Sandra E. Davidson James B. Reid Noel W. Davies Laura J. Quittenden Jason A. Smith 《Plant signaling & behavior》2012,7(12):1533-1536
Traditionally, schemes depicting auxin biosynthesis in plants have been notoriously complex. They have involved up to four possible pathways by which the amino acid tryptophan might be converted to the main active auxin, indole-3-acetic acid (IAA), while another pathway was suggested to bypass tryptophan altogether. It was also postulated that different plants use different pathways, further adding to the complexity. In 2011, however, it was suggested that one of the four tryptophan-dependent pathways, via indole-3-pyruvic acid (IPyA), is the main pathway in Arabidopsis thaliana,1 although concurrent operation of one or more other pathways has not been excluded. We recently showed that, for seeds of Pisum sativum (pea), it is possible to go one step further.2 Our new evidence indicates that the IPyA pathway is the only tryptophan-dependent IAA synthesis pathway operating in pea seeds. We also demonstrated that the main auxin in developing pea seeds, 4-chloroindole-3-acetic acid (4-Cl-IAA), which accumulates to levels far exceeding those of IAA, is synthesized via a chlorinated version of the IPyA pathway. 相似文献
6.
Non‐additive stabilization by halogenated amino acids reveals protein plasticity on a sub‐angstrom scale
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Azade S. Hosseini Christopher J. Pace Adam A. Esposito Jianmin Gao 《Protein science : a publication of the Protein Society》2017,26(10):2051-2058
It has been a long‐standing goal to understand the structure‐stability relationship of proteins, as optimal stability is essential for protein function and highly desirable for protein therapeutics. Halogenation has emerged as a minimally invasive strategy to probe the physical characteristics of proteins in solution, as well as enhance the structural stabilities of proteins for therapeutic applications. Although advances in synthetic chemistry and genetic code expansion have allowed for the rapid synthesis of proteins with diverse chemical sequences, much remains to be learned regarding the impact of these mutations on their structural integrity. In this contribution, we present a systematic study of three well‐folded model protein systems, in which their structural stabilities are assessed in response to various hydrogen‐to‐halogen atom mutations. Halogenation allows for the perturbation of proteins on a sub‐angstrom scale, offering unprecedented precision of protein engineering. The thermodynamic results from these model systems reveal that in certain cases, proteins can display modest steric tolerance to halogenation, yielding non‐additive consequences to protein stability. The observed sub‐angstrom sensitivity of protein stability highlights the delicate arrangement of a folded protein core structure. The stability data of various halogenated proteins presented herein should also provide guidelines for using halogenation as a strategy to improve the stability of protein therapeutics. 相似文献
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8.
Elnaz Khalili Samani Mohammad Reza Mofid Morteza Malakoutikhah 《Journal of peptide science》2020,26(2)
The aggregation of Aβ peptide into amyloid fibrils in the brain is associated with Alzheimer's disease (AD). Inhibition of Aβ aggregation seemed a potential treatment for AD. It was previously shown that a short fragment of Aβ peptide (KLVFF, 16‐20) bound Aβ inhibited its aggregation. In this work, using KLVFF peptide, we synthesized two peptide families and then evaluated their inhibitory capacities by conventional assays such as thioflavin T (ThT) fluorescence spectroscopy, turbidity measurement, and the 3‐(4,5‐dimethylthiazol‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H‐tetrazolium (MTS). The effect of peptide terminal groups on its inhibitory activity was first studied. Subsequently, the influence of halogenated amino acids on peptide anti‐aggregation properties was investigated. We found that iodinated peptide with amine in the N and amide in the C termini, respectively, was the best inhibitor of Aβ fibers formation. Halogenated peptides seemed to decrease the number of Aβ fibrils; however, they did not reduce Aβ cytotoxicity. The data obtained in this work seemed promising in developing potential peptide drugs for treatment of AD. 相似文献
9.
John Geigert Saul L. Neidleman Susanne K. DeWitt Demetrios J. Dalietos 《Phytochemistry》1984,23(2):287-290
Bromoperoxidases do not directly oxidize the chloride ion; nevertheless, in the presence of bromide ions, chloride ions and hydrogen peroxide, bromoperoxidases react with alkenes and alkynes to produce bromochloroderivatives. This same reaction is catalysed when seawater is the source of chloride and bromide ions. This suggests that bromonium ion-induced biosynthesis of chlorinated metabolites occurs in marine environments. The role of iodonium ions in the biosynthesis of chlorinated metabolites is also discussed. 相似文献
10.
Ethanol extraction of fresh Asparagopsis taxiformis and A. armata, followed by pentane partition, results in the isolation of a series of halomethanes, among them MeI, CHCl3, and CCl4. Under these extraction conditions, esterification readily occurs, allowing the isolation and identification of a series of polyhaloethyl acetates and acrylates. The recognition of acetone and polyhaloacetones in these extracts suggests biological halogenations occur which result in haloform reactions. 相似文献