Effect of different larval rearing temperatures on the productivity (Ro) and morphology of the malaria vector Anopheles superpictus Grassi (Diptera: Culicidae) using geometric morphometrics

Temperature affects both the biology and morphology of mosquito vectors. Geometric morphometrics is a useful new tool for capturing and analyzing differences in shape and size in many morphological parameters, including wings. We have used this technique for capturing the differences in the wings of the malaria vector Anopheles superpictus, using cohorts reared at six different constant temperatures (15°, 20°, 25°, 27°, 30°, and 35° C) and also searched for potential correlations with the life tables of the species. We studied wing shape in both male and female adults, using 22 landmarks on the wing in relation to ecological parameters, including the development rate. The ecological zero was calculated as 9.93° C and the thermal constant as 296.34 day‐degrees. The rearing temperature affects egg, larval, and pupal development and also the total time from egg to adult. As rearing temperatures increased, longevity decreased in both sexes. In An. superpictus, R_o value and productivity correlated with the statistically significant gradual deformations in the wing shape related to size in both sexes. These deformations directly linked to differences in immature rearing temperatures. Analysis using PCA and UPGMA phenograms showed that although wings of females became narrower dorsoventrally as the temperature increased, they became broader in males. Comparisons of the wing landmarks indicated the medial part of the wing was most affected by larval rearing temperatures, showing relatively more deformations. Algorithmic values of the life tables were determined in correlation with the results of geometric morphometrics. Comparisons of centroid sizes in the cohorts showed that overall wing size became smaller in both sexes in response to higher rearing temperatures.     

Clinal variation in carapace shape in the South American freshwater crab,Aegla uruguayana (Anomura: Aeglidae)

South America has been influenced by different geoclimatic events ever since its separation from Africa. The inland water fauna has evolved in response to the changing landscape. Currently, there are indications of variations in populations, occurring to different degrees that would indicate a clinal pattern in morphology. Among South America's fauna, the freshwater anomuran, Aegla, is an enigmatic group as a result of its endemicity and is composed of only one genus. Of all the species in this family, Aegla uruguayana has the broadest distribution. Its native habitats have been influenced by several marine transgressions during the Miocene–Quaternary Periods; thus, it is likely that their current distribution has been more recent. Its habitat spreads across a number of isolated basins and sub‐basins that display distinct degrees of isolation/connection, making clinal variation patterns in the morphology of this species possible. The present study aimed to evaluate the pattern of carapace shape variation in A. uruguayana and how it relates to the isolation and/or connection of populations from different basins and sub‐basins, allowing the determination of any extant clinal patterns. The specimens studied belong to 25 separate populations, representing all areas in which the species currently exists. A total of 523 crabs were analyzed. We identified 13 landmarks and four semi‐landmarks in the carapace. The aeglids were divided into seven size intervals to avoid an allometry effect. In each size category, shape relationships analyzed by principal component analysis suggest a geographical pattern corresponding to the distribution of the populations studied. An evaluation of covariation between body shape and geographical coordinates reveals a strong pattern and shows that population distribution had a significant effect on species morphology. Additionally, according to covariance analysis, the variation in shape was not associated with the environmental variables studied. We observed a clinal pattern throughout the species distribution, which could be attributed to genetic drift. It is possible that this process is being amplified by the geographical isolation of the basins, differences in environmental characteristics, and low dispersal ability. © 2014 The Linnean Society of London, Biological Journal of the Linnean Society, 2014, 113 , 914–930.     

10 000 years later: evolution of body shape in Haida Gwaii three-spined stickleback

The body shape of 1303 adult male three-spined stickleback Gasterosteus aculeatus from 118 populations on Haida Gwaii archipelago off the mid-coast of British Columbia was investigated using discriminant function analysis on partial warp scores generated from 12 homologous landmarks on a digital image of each fish. Results demonstrated geographical differences in adult body shape that could be predicted by both abiotic and biotic factors of the habitat. Populations with derived shape (CV1−), including thick peduncles, posterior and closely spaced dorsal spines, anterior pelvis, small dorsal and anal fins, were found in small, shallow, stained ponds, and populations with less derived shape (CV1+), with small narrow peduncles, anterior and widely spaced dorsal spines, posterior pelvis, large dorsal and anal fins were found in large, deep, clear lakes. This relationship was replicated between geographic regions; divergent mtDNA haplotypes in lowland populations; between predation regimes throughout the archipelago, and in each geographical region and between predation regimes in lowland populations monomorphic for the Euro and North American mtDNA haplotype. There were large-bodied populations with derived shape (CV2−), including small heads and shallow elongate bodies in open water habitats of low productivity, and populations with smaller size and less derived shape (CV2+), with large heads and deeper bodies in higher productivity, structurally complex habitats. This relationship was replicated between geographic regions, and partially between divergent mtDNA haplotypes in lowland populations. Field tests for phenotypic plasticity of body shape suggest that <10% of the total variation in body shape among populations throughout the archipelago can be attributed to plasticity.     

Three-dimensional geometric morphometrics of Arvicanthis: implications for systematics and taxonomy

Arvicanthis is an African murid, found throughout sub-Saharan Africa, Sudan and Egypt. Although in the past 10 years several studies have been carried out to assess its systematics, there is still a need for a general revision of the genus. In this study the morphometric relationships between 71 populations throughout the range were investigated. A three-dimensional geometric morphometric approach was used to assess differences in the size and shape of the skull. These were related to the different biogeographical domains characterizing the range of the genus and to molecular and karyotypic phylogenies. Results agree only in part with phylogeny, and show a close relationship with the environmental backgrounds of each species. It is therefore suggested that the adaptation of Arvicanthis to local environment has played an important role in the phenotypic evolution of the skull. This leads to problems in taxonomic definitions based on morphometrics, which should not be used without comparison with other independently derived characters such as the DNA and the karyotype.     

The biennial life strategy in a random environment

In a previous paper (J. Math. Biol. 26, 199–215 (1988)) we calculated the mean and variance of the long-run geometric growth rate of a discrete-time population model with two age classes in a random environment. The formula which was used in that paper as the starting point for the computation of the variance represents only the contribution of the one-period variances. Here we supplement these results by a calculation of the exact variance. All qualitative conclusions reached before are unaffected.     

Basicranial axis length v. skull length in analysis of carnivore skull shape

Skull length is the measurement most commonly used as a standard against which other aspects of cranial morphology are compared to derive an index of relative size or proportions. However, skull length is composed of two different functional components, facial skull and cerebral skull, which vary independently and have different scaling relationships with body size. An analysis of carnivore skull shape with measurements standardized against basicranium length produced very different results than an analysis using skull length as the standard. For example, expressions of relative size of cranial measurements were reduced by 13% in mustelids and increased by 20% in canids, reflecting removal of jaw length (short in mustelids and long in canids) from the comparative standard (basicranial axis length). Cranial measurements scale with higher allometric exponents against basicranial axis length than against skull length.     

Geographic differentiation between populations of Rhinocyllus conicus Frölich (Coleoptera: Curculionidae): concordance of allozyme and morphometric analysis

The weevil Rhinocyllu conicus develops only in the flowerheads of thistles {Asteracea; Carduineae) and therefore it is used as a biocontrol agent against thistle weed species. Populations out of 14 regions within its natural distribution area (from the Atlantic coast in the west to Israel in the east) were analysed by means of allozymes and morphometries. In both analyses the 14 populations were grouped into two concordant units. Based on nine scored loci (six of them polymorphic), mean Nei's genetic distance between the two groups was 0.073. In a reclassification test following DFA of morphometric data, 93.2% of all individuals were grouped back into the correct group. The two groups were considered to belong to a temperate and a Mediterranean subspecies respectively, the latter probably identical to the formerly described Rhinocyllus oblongus .     

Testing different 3D techniques using geometric morphometrics: Implications for cranial fluctuating asymmetry in humans

This study aimed to test the performance of 3D digitizer, CT scanner, and surface scanner in detecting cranial fluctuating asymmetry. Sets of 32 landmarks (6 in the midline and 13 bilateral) were acquired from 14 archeological crania using a 3D digitizer, and from 3D models generated from a CT scanner and surface scanner using Viewbox 4. Levels of shape variation were analyzed in MorphoJ using Procrustes analysis of variance and Principal component analysis. Intra-observer error accounted for 1.7%, 1.8%, and 4.5% of total shape variation for 3D digitizer, CT scanner, and surface scanner respectively. Fluctuating asymmetry accounted for 15%–16% of total shape variation. Variation between techniques accounted for 18% of total shape variation. We found a higher level of missing landmarks in our surface scan data than for both 3D digitizer and CT scanner data, and both 3D model-based techniques sometimes obscured taphonomic damage. All three 3D techniques are appropriate for measuring cranial fluctuating asymmetry. We advise against combining data collected with different techniques.