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1.
Summary We evaluated three methods for the analysis of functional response data by asking whether a given method could discriminate among functional responses and whether it could accurately identify regions of positive density-dependent predation. We evaluated comparative curve fitting with foraging models, linear least-squares analysis using the angular transformation, and logit analysis. Using data from nature and simulations, we found that the analyses of predation rates with the angular transformation and logit analysis were best at consistently determining the true functional response, i.e. the model used to generate simulated data. These methods also produced the most accurate estimates of the true regions of density dependence. Of these two methods, functional response data best fulfill the assumptions of logit analysis. Angularly transformed predation rates only approximate the assumptions of linear leastsquares analysis for predation rates between 0.1 and 0.9. Lack-of-fit statistics can reveal inadequate fit of a model to a data set where simple regression statistics might erroneously suggest a good match. 相似文献
2.
Dermorphin and a camel beta-endorphin (beta c-EP) analog in which residues 1-7 correspond to the dermorphin sequence ([Dermorphin1-7]-beta c-EP) have been investigated with respect to their receptor binding characteristics using human and camel beta-EP as reference peptides. Tritiated dihydromorphine, [D-Ala2, D-Leu5]-enkephalin, ethylketocyclazocine and human beta-endorphin were used as primary ligands in the rat brain membrane preparation for radioreceptor assay. Camel beta-endorphin was the most potent peptide in all experiments. [Dermorphin1-7]-beta c-EP is significantly less potent towards 3H-ethylketocyclazocine and 3H-[D-Ala2, D-Leu5]-enkephalin but is as potent towards 3H-dihydromorphine and 3H-human beta-endorphin. Dermorphin itself weakly displaces tritiated dihydromorphine, [D-Ala2, D-Leu5]-enkephalin and ethylketocyclazocine (potency relative to camel beta-EP, 1-4%) but it is more potent (9%) in competition with tritiated human beta-endorphin. Dermorphin and the [Dermorphin-1-7]-beta c-EP appear to interact preferentially with mu opiate receptors. 相似文献
3.
Maximum likelihood estimation via the ECM algorithm: A general framework 总被引:35,自引:0,他引:35
4.
Bushell ME Bell SL Scott MF Snell K Spier RE Wardell JN Sanders PG 《Biotechnology and bioengineering》1993,42(1):133-139
The use of partial cubic spline data interpolation for the calculation of volumetric metabolite exchange rates suggested the existence of three distinct metabolic phases during bioreactor culture of a hybridoma cell line. During phase 1, a rapid amino acid uptake rate and ammonia release rate were observed. The growth rate was low and glutamine synthetase activity fell. In phase 2, maximum growth rate and minimum glutamine assimilation and ammonium production rates were observed. Attempts to corroborate the apparent ammonia assimilation in this phase using (15)NH(4)Cl resulted in low incorporation rates into alanine and glutamine. Maximum glutamine synthetase activity took place during this period. Maximum antibody production rate was observed during phase 3 during which peaks in glutamine assimilation, ammonia release, and glutamine synthetase activity were observed. The apparent existence of the three phases prompted us to carry out Northern blot analysis of glutamine synthetase RNA at appropriate times during the process. This revealed a pattern of appearance and dis-appearance of mRNA consistent with the three phases indicated by the fermentation parameters. (c) 1993 John Wiley & Sons, Inc. 相似文献
5.
Glenn L. Millhauser Chris J. Stenland Kimberly A. Bolin Frank J. M. van de Ven 《Journal of biomolecular NMR》1996,7(4):331-334
Summary Alanine-rich peptides serve as models for exploring the factors that control helix structure in peptides and proteins. Scalar CH-NH couplings (3JHN) are an extremely useful measure of local helix content; however, the large alanine content in these peptides leads to significant signal overlap in the CH region of 1H 2D NMR spectra. Quantitative determination of all possible 3JHN values is, therefore, very challenging. Szyperski and co-workers [(1992) J. Magn. Reson., 99, 552–560] have recently developed a method for determining 3JHN from NOESY spectra. Because 3JHN may be determined from 2D peaks outside of the CH region, there is a much greater likelihood of identifying resolved resonances and measuring the associated coupling constants. It is demonstrated here that 3JHN can be obtained for every residue in the helical peptide Ac-(AAAAK)3A-NH2. The resulting 3JHN profile clearly identifies a helical structure in the middle of the peptide and further suggests that the respective helix termini unfold via distinct pathways.Abbreviations
3JHN
three-bond CH-NH scalar coupling constant
- NOE
nuclear Overhauser enhancement
- NOESY
two-dimensional nuclear Overhauser spectroscopy
- COSY
two-dimensional correlated spectroscopy
- DQF-COSY
two-dimensional double-quantum-filtered correlated spectroscopy
- TOCSY
two-dimensional total correlation spectroscopy
To whom correspondence should be addressed.Deceased March 5, 1996. 相似文献
6.
7.
The rapid development of the concept of the “Internet of Things (IoT)” requires wearable devices with maintenance‐free batteries, and thermoelectric energy conversion based on large‐area flexible materials has attracted much attention. Among large‐area flexible materials, 2D materials, such as graphene and related materials, are promising for thermoelectric applications due to their excellent transport properties and large power factors. In this Review, both single‐crystalline and polycrystalline 2D materials are surveyed using the experimental reports on thermoelectric devices of graphene, black phosphorus, transition metal dichalcogenides, and other 2D materials. In particular, their carrier‐density dependent thermoelectric properties and power factors maximized by Fermi level tuning techniques are focused. The comparison of the relevant performances between 2D materials and commonly used thermoelectric materials reveals the significantly enhanced power factors in 2D materials. Moreover, the current progress in thermoelectric module applications using large‐area 2D material thin films is summarized, which consequently offers great potential for the use of 2D materials in large‐area flexible thermoelectric device applications. Finally, important remaining issues and future perspectives, such as preparation methods, thermal transports, device designs, and promising effects in 2D materials, are discussed. 相似文献
8.
Geon‐Hyoung An John Hong Sangyeon Pak Yuljae Cho Sanghyo Lee Bo Hou SeungNam Cha 《Liver Transplantation》2020,10(3)
Recent supercapacitors show a high power density with long‐term cycle life time in energy‐powering applications. A supercapacitor based on a single metal electrode accompanying multivalent cations, multiple charging/discharging kinetics, and high electrical conductivity is a promising energy‐storing system that replaces conventionally used oxide and sulfide materials. Here, a hierarchically nanostructured 2D‐Zn metal electrode‐ion supercapacitor (ZIC) is reported which significantly enhances the ion diffusion ability and overall energy storage performance. Those nanostructures can also be successfully plated on various flat‐type and fiber‐type current collectors by a controlled electroplating method. The ZIC exhibits excellent pseudocapacitive performance with a high energy density of 208 W h kg?1 and a power density from 500 W kg?1, which are significantly higher than those of previously reported supercapacitors with oxide and sulfide materials. Furthermore, the fiber‐type ZIC also shows high energy‐storing performance, outstanding mechanical flexibility, and waterproof characteristics, without any significant capacitance degradation during bending tests. These results highlight the promising possibility of nanostructured 2D Zn metal electrodes with the controlled electroplating method for future energy storage applications. 相似文献
9.
Mengran Wang Yuan Li Jing Fang Cesar J. Villa Yaobin Xu Shiqiang Hao Jie Li Yexiang Liu Chris Wolverton Xinqi Chen Vinayak P. Dravid Yanqing Lai 《Liver Transplantation》2020,10(3)
Carbon dots have been recognized as one of the most promising candidates for the oxygen reduction reaction (ORR) in alkaline media. However, the desired ORR performance in metal–air batteries is often limited by the moderate electrocatalytic activity and the lack of a method to realize good dispersion. To address these issues, herein a biomass‐deriving method is reported to achieve the in situ phosphorus doping (P‐doping) of carbon dots and their simultaneous decoration onto graphene matrix. The resultant product, namely P‐doped carbon dot/graphene (P‐CD/G) nanocomposites, can reach an ultrahigh P‐doping level for carbon nanomaterials. The P‐CD/G nanocomposites are found to exhibit excellent ORR activity, which is highly comparable to the commercial Pt/C catalysts. When used as the cathode materials for a primary liquid Al–air battery, the device shows an impressive power density of 157.3 mW cm?2 (comparing to 151.5 mW cm?2 of a similar Pt/C battery). Finally, an all‐solid‐state flexible Al–air battery is designed and fabricated based on our new nanocomposites. The device exhibits a stable discharge voltage of ≈1.2 V upon different bending states. This study introduces a unique biomass‐derived material system to replace the noble metal catalysts for future portable and wearable electronic devices. 相似文献
10.
Zhen Li Shengfan Wu Jie Zhang Ka Chun Lee Hang Lei Francis Lin Zilong Wang Zonglong Zhu Alex K. Y. Jen 《Liver Transplantation》2020,10(18)
Perovskite‐organic tandem solar cells are attracting more attention due to their potential for highly efficient and flexible photovoltaic device. In this work, efficient perovskite‐organic monolithic tandem solar cells integrating the wide bandgap perovskite (1.74 eV) and low bandgap organic active PBDB‐T:SN6IC‐4F (1.30 eV) layer, which serve as the top and bottom subcell, respectively, are developed. The resulting perovskite‐organic tandem solar cells with passivated wide‐bandgap perovskite show a remarkable power conversion efficiency (PCE) of 15.13%, with an open‐circuit voltage (Voc) of 1.85 V, a short‐circuit photocurrent (Jsc) of 11.52 mA cm?2, and a fill factor (FF) of 70.98%. Thanks to the advantages of low temperature fabrication processes and the flexibility properties of the device, a flexible tandem solar cell which obtain a PCE of 13.61%, with Voc of 1.80 V, Jsc of 11.07 mA cm?2, and FF of 68.31% is fabricated. Moreover, to demonstrate the achieved high Voc in the tandem solar cells for potential applications, a photovoltaic (PV)‐driven electrolysis system combing the tandem solar cell and water splitting electrocatalysis is assembled. The integrated device demonstrates a solar‐to‐hydrogen efficiency of 12.30% and 11.21% for rigid, and flexible perovskite‐organic tandem solar cell based PV‐driven electrolysis systems, respectively. 相似文献