排序方式: 共有20条查询结果,搜索用时 31 毫秒
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Jonathan C. Guito Joseph B. Prescott Catherine E. Arnold Brian R. Amman Amy J. Schuh Jessica R. Spengler Tara K. Sealy Jessica R. Harmon JoAnn D. Coleman-McCray Kirsten A. Kulcsar Elyse R. Nagle Raina Kumar Gustavo F. Palacios Mariano Sanchez-Lockhart Jonathan S. Towner 《Current biology : CB》2021,31(2):257-270.e5
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Filoviruses have to date been considered as consisting of one diverse genus (Ebola viruses) and one undifferentiated genus (Marburg virus). We reconsider this idea by means of detailed phylogenetic analyses of sequence data available for the Filoviridae: using coalescent simulations, we ascertain that two Marburg isolates (termed the "RAVN" strain) represent a quite-distinct lineage that should be considered in studies of biogeography and host associations, and may merit recognition at the level of species. In contrast, filovirus isolates recently obtained from bat tissues are not distinct from previously known strains, and should be considered as drawn from the same population. Implications for understanding the transmission geography and host associations of these viruses are discussed. 相似文献
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《Cell reports》2020,30(2):308-319.e5
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Kachko A. V. Cheusova T. B. Sorokin A. V. Kazachinskaya E. I. Cheshenko I. O. Belanov E. F. Bukreev A. A. Ivanova A. V. Razumov I. A. Ryabchikova E. I. Netesov S. V. 《Molecular Biology》2001,35(3):417-422
The full-length gene for Marburg virus (MV) nucleoprotein (NP) was cloned in prokaryotic pQE32 under the control of the T5 promoter and in eukaryotic pTM1 under the control of the T7 RNA polymerase promoter. Recombinant NP was synthesized in Escherichia coliand in human kidney cell line 293 cotransfected with recombinant vaccinia virus vTF7-3 expressing T7 RNA polymerase. On evidence of electron microscopy with immune detection, recombinant NP formed tubules of two types in E. coliand of a single type in cell line 293. ELISA and immunoblotting with polyclonal and monoclonal antibodies revealed common antigenic determinants in recombinant NP and natural MV NP. 相似文献
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T.J. Piercy S.J. Smither J.A. Steward L. Eastaugh M.S. Lever 《Journal of applied microbiology》2010,109(5):1531-1539
Aims: Filoviruses are associated with high morbidity and lethality rates in humans, are capable of human‐to‐human transmission, via infected material such as blood, and are believed to have low infectious doses for humans. Filoviruses are able to infect via the respiratory route and are lethal at very low doses in experimental animal models, but there is minimal information on how well the filoviruses survive within aerosol particles. There is also little known about how well filoviruses survive in liquids or on solid surfaces which is important in management of patients or samples that have been exposed to filoviruses. Methods and Results: Filoviruses were tested for their ability to survive in different liquids and on different solid substrates at different temperatures. The decay rates of filoviruses in a dynamic aerosol were also determined. Conclusions: Our study has shown that Lake Victoria marburgvirus (MARV) and Zaire ebolavirus (ZEBOV) can survive for long periods in different liquid media and can also be recovered from plastic and glass surfaces at low temperatures for over 3 weeks. The decay rates of ZEBOV and Reston ebolavirus (REBOV) plus MARV within a dynamic aerosol were calculated. ZEBOV and MARV had similar decay rates, whilst REBOV showed significantly better survival within an aerosol. Significance and Impact of the Study: Data on the survival of two ebolaviruses are presented for the first time. Extended data on the survival of MARV are presented. Data from this study extend the knowledge on the survival of filoviruses under different conditions and provide a basis with which to inform risk assessments and manage exposure to filoviruses. 相似文献
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Design and characterization of ebolavirus GP prehairpin intermediate mimics as drug targets 下载免费PDF全文
Tracy R Clinton Matthew T Weinstock Michael T Jacobsen Nicolas Szabo-Fresnais Maya J Pandya Frank G Whitby Andrew S Herbert Laura I Prugar Rena McKinnon Christopher P Hill Brett D Welch John M Dye Debra M Eckert Michael S Kay 《Protein science : a publication of the Protein Society》2015,24(4):446-463
Ebolaviruses are highly lethal filoviruses that cause hemorrhagic fever in humans and nonhuman primates. With no approved treatments or preventatives, the development of an anti-ebolavirus therapy to protect against natural infections and potential weaponization is an urgent global health need. Here, we describe the design, biophysical characterization, and validation of peptide mimics of the ebolavirus N-trimer, a highly conserved region of the GP2 fusion protein, to be used as targets to develop broad-spectrum inhibitors of ebolavirus entry. The N-trimer region of GP2 is 90% identical across all ebolavirus species and forms a critical part of the prehairpin intermediate that is exposed during viral entry. Specifically, we fused designed coiled coils to the N-trimer to present it as a soluble trimeric coiled coil as it appears during membrane fusion. Circular dichroism, sedimentation equilibrium, and X-ray crystallography analyses reveal the helical, trimeric structure of the designed N-trimer mimic targets. Surface plasmon resonance studies validate that the N-trimer mimic binds its native ligand, the C-peptide region of GP2. The longest N-trimer mimic also inhibits virus entry, thereby confirming binding of the C-peptide region during viral entry and the presence of a vulnerable prehairpin intermediate. Using phage display as a model system, we validate the suitability of the N-trimer mimics as drug screening targets. Finally, we describe the foundational work to use the N-trimer mimics as targets in mirror-image phage display, which will be used to identify d-peptide inhibitors of ebolavirus entry. 相似文献
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