外切纤维素酶的研究与应用进展

纤维素酶主要包括三大类：外切纤维素酶、内切纤维素酶和β-葡萄糖苷酶。其中,外切纤维素酶因具有活性高、耐受性好、来源广等特点而被广泛应用于各种工业生产中。基于此,主要阐述了外切纤维素酶的分类、来源以及生化特性,介绍了外切纤维素酶的筛选及其水解产物分析的相关新技术,并综述了其在造纸业、食品加工业、制药业、纺织业、能源再生等工业领域中的应用进展,以期推动外切纤维素酶的深入研究及工业化应用。     

Preparation of cellulase concoction using differential adsorption phenomenon

Controlled depolymerization of cellulose is essential for the production of valuable cellooligosaccharides and cellobiose from lignocellulosic biomass. However, enzymatic cellulose hydrolysis involves multiple synergistically acting enzymes, making difficult to control the depolymerization process and generate desired product. This work exploits the varying adsorption properties of the cellulase components to the cellulosic substrate and aims to control the enzyme activity. Cellulase adsorption was favored on pretreated cellulosic biomass as compared to synthetic cellulose. Preferential adsorption of exocellulases was observed over endocellulase, while β-glucosidases remained unadsorbed. Adsorbed enzyme fraction with bound exocellulases when used for hydrolysis generated cellobiose predominantly, while the unadsorbed enzymes in the liquid fraction produced cellooligosaccharides majorly, owing to its high endocellulases activity. Thus, the differential adsorption phenomenon of the cellulase components can be used for the controlling cellulose hydrolysis for the production of an array of sugars.