全文获取类型
收费全文 | 1009篇 |
免费 | 97篇 |
国内免费 | 112篇 |
出版年
2024年 | 8篇 |
2023年 | 40篇 |
2022年 | 33篇 |
2021年 | 70篇 |
2020年 | 57篇 |
2019年 | 74篇 |
2018年 | 85篇 |
2017年 | 51篇 |
2016年 | 49篇 |
2015年 | 71篇 |
2014年 | 139篇 |
2013年 | 108篇 |
2012年 | 63篇 |
2011年 | 44篇 |
2010年 | 38篇 |
2009年 | 46篇 |
2008年 | 43篇 |
2007年 | 31篇 |
2006年 | 23篇 |
2005年 | 23篇 |
2004年 | 12篇 |
2003年 | 14篇 |
2002年 | 10篇 |
2001年 | 12篇 |
2000年 | 9篇 |
1999年 | 6篇 |
1998年 | 9篇 |
1997年 | 2篇 |
1996年 | 8篇 |
1995年 | 4篇 |
1994年 | 3篇 |
1993年 | 6篇 |
1992年 | 3篇 |
1991年 | 5篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 3篇 |
1978年 | 1篇 |
1977年 | 1篇 |
排序方式: 共有1218条查询结果,搜索用时 15 毫秒
1.
《Developmental cell》2021,56(21):2995-3005.e4
- Download : Download high-res image (168KB)
- Download : Download full-size image
2.
A main problem in the vegetative propagation of ornamental plants in vitro is the epigenetic instability of cells removed from their organized environment. With calluses of leaf explants of Kalanchoe blossfeldiana Poelln., cv. Yucatan, the role of plant growth regulators (PGRs) in the occurrence of fasciation was studied.In various combinations of auxins and cytokinins, the auxin 2,4-D (2,4-dichlorophenoxyacetic acid) gave only deformed, inseparable shoot primordia. The most rapid callus induction with regeneration of well-developed sprouts was obtained with the natural IAA (indoleacetic acid) and Z (zeatin).As a first symptom of fasciation, aberration in decussate phyllotaxis can be observed. At increasing concentrations of IAA + Z, this symptom gradually decreased but fasciation proper increased. The optimum concentration was at 1 M for both PGRs. Reduction of exposure to the PGRs from six to three weeks reduced the epigenetic instability. 相似文献
3.
J. E. Irvine G. T. A. Benda B. L. Legendre G. R. Machado Jr 《Plant Cell, Tissue and Organ Culture》1991,26(2):115-125
This study investigates the frequency of apparent and permanent expression of marker change following two types of tissue culture, conventional callus and direct regeneration cultures, and for two markers it relates this frequency to that following breeding. Each clone was used for only one marker. After conventional callus culture, plants of the sugarcane clone Arundoid B, a clone having a growth habit with shortened internodes and leaves, were freed of this marker at a rate of 1 in 172 plants. Marker remission in a second clone with a leaf blotch was enhanced in the presence of a mutagen. Callus culture alone gave a remission rate of 1/280 plants, while treatment of callus with ethyl methanesulfonate gave a remission rate of 1/42 plants. Of two markers subjected to vegetative and sexual transmission, the first, a leaf marker, was stable in callus culture with no remissions; crossing with non-marker parents produced progeny with 54% lacking the marker. The second, a stalk marker (multibud), showed epigenetic effects during two generations of vegetative propagation; plants lacking the multibud marker produced vegetative progeny in which the marker reappeared. Nine crosses to nonmarker parents produced progeny of which an average of 29% had the marker. The use of stalk chimeras as markers demonstrated that passage through conventional callus or direct regeneration culture resulted in the loss of the donor phenotype in all plants regenerated. Phenotypic variation in plants derived from callus culture appears to arise from several sources; chimeral segregants, epigenetic transients, and mutational variants. 相似文献
4.
5.
Michael Turelli 《Evolution; international journal of organic evolution》1994,48(5):1500-1513
In many insect species, males infected with microbes related to Wolbachia pipientis are “incompatible” with uninfected females. Crosses between infected males and uninfected females produce significantly fewer adult progeny than the other three possible crosses. The incompatibility-inducing microbes are usually maternally transmitted. Thus, incompatibility tends to confer a reproductive advantage on infected females in polymorphic populations, allowing these infections to spread. This paper analyzes selection on parasite and host genes that affect such incompatibility systems. Selection among parasite variants does not act directly on the level of incompatibility with uninfected females. In fact, selection favors rare parasite variants that increase the production of infected progeny by infected mothers, even if these variants reduce incompatibility with uninfected females. However, productivity-reducing parasites that cause partial incompatibility with hosts harboring alternative variants can be favored once they become sufficiently abundant locally. Thus, they may spread spatially by a process analogous to the spread of underdominant chromosome rearrangements. The dynamics of modifier alleles in the host are more difficult to predict, because such alleles will occur in both infected and uninfected individuals. Nevertheless, the relative fecundity of infected females compared to uninfected females, the efficiency of maternal transmission and the mutual compatibility of infected individuals all tend to increase under within-population selection on both host and parasite genes. In addition, selection on host genes favors increased compatibility between infected males and uninfected females. Although vertical transmission tends to harmonize host and parasite evolution, competition among parasite variants will tend to maintain incompatibility. 相似文献
6.
7.
表观遗传调控是真核生物基因表达精细调控的重要组成部分,主要包括DNA甲基化、组蛋白修饰和染色质重塑。其中,染色质重塑因子可影响组蛋白修饰酶和转录因子与特定位点的结合,在基因表达调控中占有重要地位。INO80复合物是进化上保守的染色质重塑复合物,能利用ATP水解获得的能量促进核小体的滑动和驱逐。INO80复合物除了在DNA复制、修复中发挥重要功能外,还通过改变DNA可及性调控酿酒酵母的基因表达。本文综述了染色质重塑复合物的分类及组成,重点介绍了酿酒酵母多亚基复合物INO80在基因表达调控中的重要功能,包括驱逐RNA聚合酶Ⅱ、响应信号转导途径和改变基因表达水平等,并着重总结了其在酿酒酵母环境胁迫响应机理中的研究进展。深入研究INO80染色质重塑复合物的功能,可为理解真核生物精细代谢调控的机制,并进一步开发基于染色质重塑等表观调控水平的微生物代谢工程和合成生物学改造策略,提高菌株的环境胁迫耐受性和发酵性能提供基础。 相似文献
8.
9.
Steven T. Gardner Emily M. Bertucci Randall Sutton Andy Horcher Doug Aubrey Benjamin B. Parrott 《Molecular ecology resources》2023,23(1):131-144
Biological ageing is connected to life history variation across ecological scales and informs a basic understanding of age-related declines in organismal function. Altered DNA methylation dynamics are a conserved aspect of biological ageing and have recently been modelled to predict chronological age among vertebrate species. In addition to their utility in estimating individual age, differences between chronological and predicted ages arise due to acceleration or deceleration of epigenetic ageing, and these discrepancies are linked to disease risk and multiple life history traits. Although evidence suggests that patterns of DNA methylation can describe ageing in plants, predictions with epigenetic clocks have yet to be performed. Here, we resolve the DNA methylome across CpG, CHG, and CHH-methylation contexts in the loblolly pine tree (Pinus taeda) and construct epigenetic clocks capable of predicting ages in this species within 6% of its maximum lifespan. Although patterns of CHH-methylation showed little association with age, both CpG and CHG-methylation contexts were strongly associated with ageing, largely becoming hypomethylated with age. Among age-associated loci were those in close proximity to malate dehydrogenase, NADH dehydrogenase, and 18S and 26S ribosomal RNA genes. This study reports one of the first epigenetic clocks in plants and demonstrates the universality of age-associated DNA methylation dynamics which can inform conservation and management practices, as well as our ecological and evolutionary understanding of biological ageing in plants. 相似文献
10.