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Tadao Kitagawa Motoi Yoshioka Masaaki Kashiwagi Toshio Okazaki 《Ichthyological Research》2001,48(2):127-135
Population structures of the delicate loach, Niwaella delicata, were inferred from morphology and restriction fragment length polymorphism (RFLP) analysis of part of the mitochondrial
DNA (mtDNA) of 25 populations, representing the species range in central Honshu Island. The existence of two types of morphological
variation corresponding to regional distributions, the "Pacific slope type" and "Sea of Japan slope type," has been known
in N. delicata. Our morphological reexamination of the two types revealed some discrepancies in their distribution pattern. Therefore, we
reclassified two new color types corresponded to their distribution areas as "gathered spots type (G type)" and "scattered
spots type (S type)," respectively. The present classification of G and S types is closely related to the mtDNA divergence
pattern. The current analysis also indicated that each G and S type population was further divided into two genetic groups,
corresponding to geographic proximity. In spite of marked morphological differentiation, the genetic diversity between G and
S type populations (1.153%) was comparable only to that reported for intraspecific levels in most freshwater fishes. Moreover,
in the population of which the color patterns of all fish were characterized to the S type, mtDNA haplotypes corresponding
to G and S types were sympatrically detected. This result indicates secondary contact between the two type populations and
the possibility that they are not reproductively isolated.
Received: June 11, 1999 / Revised: September 30, 2000 / Accepted: January 16, 2001 相似文献
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采用水分抗逆性指标主成分分析法,选择4年实生苗,测定和评价了连续30 d水涝和干旱胁迫的生理响应.结果表明:(1)水涝处理的叶片生长旺盛,光泽度和观赏性无明显变化,叶片相对含水量、MDA含量、SOD活性、POD活性、CAT活性的变化差异在5%以内,土壤含水量增加3.47%,细胞膜透性减少1.59%,短期水涝对美丽红山茶伤害不明显;(2)随着干旱时间延长,新叶卷缩、老叶脱落,土壤含水量、叶片相对含水量随时间呈负相关缓慢减少,细胞膜透性呈正相关缓慢增长,但复水10 d 后可恢复正常生长,无明显生理变化响应;(3)SOD活性和MDA含量分别增加了0.66%和5.31%,POD活性随干旱时间上下波动而增加6.47%, CAT活性随干旱时间延长而增加,20 d增加33.33%达最大值20.8 U??g-1??min-1.在抗性生理中,维持膜稳定性的叶片含水量、POD和CAT的变化对抗旱能力大小起主要作用. 相似文献
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Masao Kimura 《Animal genetics》1978,9(3):183-186
Hemoglobin, non-enzymatic and enzymatic muscle proteins of the loach Cobitis delicata were examined electrophoretically. Eleven proteins were shown to be under control by 20 loci. Only two loci were defined as polymorphic and 14 loci were completely monomorphic. Estimated average heterozygosity over all 20 loci was 0.011 in the loach population. 相似文献
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