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Karla Lopes Mandu de Campos Ana Paula Machado Flávia Gatto de Almeida Camila Mareti Bonin Thiago Theodoro Martins Prata Larissa Zatorre Almeida Cacilda Tezelli Junqueira Padovani Alda Maria Teixeira Ferreira Carlos Eurico dos Santos Fernandes Inês Aparecida Tozetti 《Memórias do Instituto Oswaldo Cruz》2014,109(3):352-355
Women infected with human papillomavirus (HPV) are at a higher risk of developing
cervical lesions. In the current study, self and clinician-collected vaginal and
cervical samples from women were processed to detect HPV DNA using polymerase chain
reaction (PCR) with PGMY09/11 primers. HPV genotypes were determined using
type-specific PCR. HPV DNA detection showed good concordance between self and
clinician-collected samples (84.6%; kappa = 0.72). HPV infection was found in 30%
women and genotyping was more concordant among high-risk HPV (HR-HPV) than low-risk
HPV (HR-HPV). HPV16 was the most frequently detected among the HR-HPV types. LR-HPV
was detected at a higher frequency in self-collected; however, HR-HPV types were more
frequently identified in clinician-collected samples than in self-collected samples.
HPV infections of multiple types were detected in 20.5% of clinician-collected
samples and 15.5% of self-collected samples. In this study, we demonstrated that the
HPV DNA detection rate in self-collected samples has good agreement with that of
clinician-collected samples. Self-collected sampling, as a primary prevention
strategy in countries with few resources, could be effective for identifying cases of
HR-HPV, being more acceptable. The use of this method would enhance the coverage of
screening programs for cervical cancer. 相似文献
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