全文获取类型
收费全文 | 5035篇 |
免费 | 307篇 |
国内免费 | 241篇 |
出版年
2024年 | 12篇 |
2023年 | 62篇 |
2022年 | 128篇 |
2021年 | 136篇 |
2020年 | 143篇 |
2019年 | 192篇 |
2018年 | 194篇 |
2017年 | 145篇 |
2016年 | 139篇 |
2015年 | 183篇 |
2014年 | 247篇 |
2013年 | 408篇 |
2012年 | 212篇 |
2011年 | 284篇 |
2010年 | 188篇 |
2009年 | 260篇 |
2008年 | 262篇 |
2007年 | 299篇 |
2006年 | 256篇 |
2005年 | 234篇 |
2004年 | 221篇 |
2003年 | 175篇 |
2002年 | 163篇 |
2001年 | 121篇 |
2000年 | 106篇 |
1999年 | 140篇 |
1998年 | 80篇 |
1997年 | 83篇 |
1996年 | 71篇 |
1995年 | 69篇 |
1994年 | 65篇 |
1993年 | 50篇 |
1992年 | 48篇 |
1991年 | 34篇 |
1990年 | 17篇 |
1989年 | 19篇 |
1988年 | 17篇 |
1987年 | 9篇 |
1986年 | 11篇 |
1985年 | 16篇 |
1984年 | 13篇 |
1983年 | 10篇 |
1982年 | 13篇 |
1981年 | 14篇 |
1979年 | 6篇 |
1978年 | 7篇 |
1977年 | 5篇 |
1976年 | 4篇 |
1974年 | 3篇 |
1973年 | 5篇 |
排序方式: 共有5583条查询结果,搜索用时 15 毫秒
1.
2.
Monica Driscoll 《Developmental neurobiology》1992,23(9):1327-1351
In C. elegans, cell death can be readily studied at the cellular, genetic, and molecular levels. Two types of death have been characterized in this nematode: (1) programmed cell death, which occurs as a normal component in development; and (2) pathological cell death which occurs aberrantly as a consequence of mutation. Analysis of mutations that disrupt programmed cell death in various ways has defined a genetic pathway for programmed cell death which includes genes that perform such functions as the determination of which cells die, the execution of cell death, the engulfment of cell corpses, and the digestion of DNA from dead cells. Molecular analysis is providing insightinto the nature of the molecules that function in these aspects of programmed cell death. Characterization of some genes that mutate to induce abnormal cell death has defined a novel gene family called degenerins that encode putative membrane proteins. Dominant alleles of at least two degenerin genes, mec-4 and deg-1, can cause cellular swelling and late onset neurodegeneration of specific groups of cells. © 1992 John Wiley & Sons, Inc. 相似文献
3.
G. Franz M. Reindl S. C. Patel R. Beer I. Unterrichter T. Berger E. Schmutzhard W. Poewe & A. Kampfl 《Journal of neurochemistry》1999,73(4):1615-1625
Increasing evidence suggests that apolipoprotein D (apoD) could play a major role in mediating neuronal degeneration and regeneration in the CNS and the PNS. To investigate further the temporal pattern of apoD expression after experimental traumatic brain injury in the rat, male Sprague-Dawley rats were subjected to unilateral cortical impact injury. The animals were killed and examined for apoD mRNA and protein expression and for immunohistological analysis at intervals from 15 min to 14 days after injury. Increased apoD mRNA and protein levels were seen in the cortex and hippocampus ipsilateral to the injury site from 48 h to 14 days after the trauma. Immunohistological investigation demonstrated a differential pattern of apoD expression in the cortex and hippocampus, respectively: Increased apoD immunoreactivity in glial cells was detected from 2 to 3 days after the injury in cortex and hippocampus. In contrast, increased expression of apoD was seen in cortical and hippocampal neurons at later time points following impact injury. Concurrent histopathological examination using hematoxylin and eosin demonstrated dark, shrunken neurons in the cortex ipsilateral to the injury site. In contrast, no evidence of cell death was observed in the hippocampus ipsilateral to the injury site up to 14 days after the trauma. No evidence of increased apoD mRNA or protein expression or neuronal pathology by hematoxylin and eosin staining was detected in the contralateral cortex and hippocampus. Our results reveal induction of apoD expression in the cortex and hippocampus following traumatic brain injury in the rat. Our data also suggest that increased apoD expression may play an important role in cortical neuronal degeneration after brain injury in vivo. However, increased expression of apoD in the hippocampus may not necessarily be indicative of neuronal death. 相似文献
4.
Antonis E. Koromilas 《Biochimica et Biophysica Acta (BBA)/General Subjects》2019,1863(3):644-649
Cells employ pro-survival and pro-adaptive pathways to cope with different forms of environmental stress. When stress is excessive, and the damage caused by it is unsustainable, cells engage pro-death pathways, which are in place to protect the host from the deleterious effects of harmed cells. Two important pathways that determine the balance between survival and death of stressed cells are the integrated stress response (ISR) and the mammalian target of rapamycin (mTOR), both of which converge at the level of mRNA translation. The two pathways have established avenues of communication to control their activity and determine the fate of stressed cells in a context-dependent manner. The functional interplay between the ISR and mTOR may have significant ramifications in the development and treatment of human diseases such as diabetes, neurodegeneration and cancer. 相似文献
5.
6.
《Developmental cell》2020,52(6):714-730.e5
- Download : Download high-res image (188KB)
- Download : Download full-size image
7.
8.
L. Chaerle F. De Boever M. Van Montagu & D. Van Der Straeten 《Plant, cell & environment》2001,24(1):15-25
Pending cell death was visualized by thermographic imaging in bacterio‐opsin transgenic tobacco plants. Cell death in these plants was characterized by a complex lesion phenotype. Isolated cell death lesions were preceded by a colocalized thermal effect, as previously observed at sites infected by tobacco mosaic virus (TMV) ( Chaerle et al. 1999 Nature Biotechnology 17, 813–816). However, in most cases, a coherent front of higher temperature, trailed by cell death, initiated at the leaf base and expanded over the leaf lamina. In contrast to the homogenous thermal front, cell death was first visible close to the veins, and subsequently appeared as discrete spots on the interveinal tissue, as cell death spread along the veins. Regions with visible cell death had a lower temperature because of water evaporation from damaged cells. In analogy with previous observations on the localized tobacco–TMV interaction ( Chaerle et al. 1999 ), the kinetics of thermographic and continuous gas exchange measurements indicated that stomatal closure preceded tissue collapse. Localized spontaneous cell death could also be presymptomatically visualized in the Arabidopsis lsd2 mutant. 相似文献
9.
Little is known about the biological functions of the phospholipase A2 receptor (PLA2R1) except that it has the ability to bind a few secreted phospholipases A2 (sPLA2′s). We have previously shown that PLA2R1 regulates senescence in normal human cells. In this study, we investigated the ability of PLA2R1 to control cancer cell growth. Analysis of expression in cancer cells indicates a marked PLA2R1 decrease in breast cancer cell lines compared to normal or nontransformed human mammary epithelial cells. Accordingly, PLA2R1 ectopic expression in PLA2R1-negative breast cancer cell lines led to apoptosis, whereas a prosenescence response was predominantly triggered in normal cells. PLA2R1 structure–function studies and the use of chemical inhibitors of sPLA2-related signaling pathways suggest that the effect of PLA2R1 is sPLA2-independent. Functional experiments demonstrate that PLA2R1 regulation of cell death is driven by a reactive oxygen species (ROS)-dependent mechanism. While screening for ROS-producing complexes involved in PLA2R1 biological responses, we identified a critical role for the mitochondrial electron transport chain in PLA2R1-induced ROS production and cell death. Taken together, this set of data provides evidence for an important role of PLA2R1 in controlling cancer cell death by influencing mitochondrial biology. 相似文献
10.
《Molecular cell》2020,77(4):748-760.e9
- Download : Download high-res image (218KB)
- Download : Download full-size image