Formaldehyde dehydrogenase from the recombinant yeast Hansenula polymorpha: isolation and bioanalytic application

A recombinant yeast clone, a derivative of the recipient Hansenula polymorpha strain NCYC 495, was chosen as an NAD and glutathione-dependent formaldehyde dehydrogenase overproducer. Optimal cultivation conditions for the highest yield of enzyme were established. A simple scheme for the isolation of formaldehyde dehydrogenase from the recombinant strain was proposed, and some characteristics of the purified enzyme were studied. An enzymatic method for formaldehyde assay based on formaldehyde dehydrogenase was developed and used for testing real samples.     

Three Novel Gibberellins Produced by Gibberella fujikuroi

Three novel gibberellins, GA₅₄ (ent-1α, 3α, 10-trihydroxy-20-norgibberell-16-ene-7, 19-dioic acid 19, 10-lactone), GA₅₅ (ent-1α, 3α, 10, 13-tetrahydroxy-20-norgibberell-16-ene-7, 19-dioic acid 19, 10-lactone) and GA₅₆ (ent-2β, 3α, 10, 13-tetrahydroxy-20-norgibberell-16-ene-7, 19-dioic acid 19, 10-lactone) were shown to occur in the culture broth of Gibberella fujikuroi. Their structures were determined mainly by mass spectrometrical comparison of the derivatives with those of authentic compounds prepared from known gibberellins.     

长白红景天天然种群遗传多样性及遗传分化

本研究利用水平淀粉凝胶电泳技术对分布于黑龙江省大海林业业局平顶山及吉林省的长白山北坡的四个海拔梯度的长白红景天的遗传多样性及遗传分化进行了初步分析,     

Heterologous expression of a deuterated membrane-integrated receptor and partial deuteration in methylotrophic yeasts

Methylotrophic yeast has previously been shown to be an excellent system for the cost-effective production of perdeuterated biomass and for the heterologous expression of membrane receptors. A protocol for the expression of 85% deuterated, functional human -opiate receptor was established. For partially deuterated biomass, deuteration level and distribution were determined for fatty acids, amino acids and carbohydrates. It was shown that prior to biosynthesis of lipids and amino acids (and of carbohydrates, to a lower extent), exchange occurs between water and methanol hydrogen atoms, so that 80%–90% randomly deuterated biomass and over-expressed proteins may be obtained using only deuterated water.     

越桔两种蛀干害虫的发生与防治

咖啡豹蠹蛾Zeuzera coffeae Nietner和樟泥色天牛Uraecha angusta(Pascoe)是江苏地区的美国兔眼越桔上2种主要蛀干害虫。前者在枝干中向顶端方向蛀食,排出圆粒状虫粪;后者向植株基部方向蛀食,排出粉末状虫粪,均造成枝干枯死。咖啡豹蠹蛾发生量较大,是主要虫种。防治这2种蛀干害虫,应采用保护利用天敌、改善果园生态环境、促使树体健壮生长等综合管理措施。     

天麻及其近缘植物酚性成分的高效液相色谱定量分析

本文应用高效液相色谱技术对天麻(Gastrodia elata Blume)及其近缘植物共16个样品的8种酚性成分进行定性定量分析。实验结果表明,该方法不仅可用于检验中药天麻的质量、亦可用于筛选新的天麻素药物资源,并为其化学分类提供一定的依据。