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1.
Stephen C. Fry 《Physiologia plantarum》1989,75(4):532-536
The plant growth promoter, auxin, may loosen the primary cell wall by increasing the activity of extracellular cellulases – a group of enzymes that cleave hemicellulose chains in the walls of both monocotyledons and dicotyledons. Evidence is reviewed that suggests that these hemicellulose chains tether adjacent microfibrils, and that by cleaving such chains the cellulases facilitate cell expansion. On the basis of this structural arrangement a mechanism for elastic and plastic wall extension is proposed. 相似文献
2.
G. -J. A. Vidugiris A. V. Gudavičius V. J. Razumas J. J. Kulys 《European biophysics journal : EBJ》1989,17(1):19-23
Surface enhanced Raman scattering (SERS) of some enzymes (alkaline phosphatase, horseradish peroxidase and lactoperoxidase) and some amino acids (tryptophan, tyrosine and phenylalanine) on silver electrodes has been studied. The spectral band intensities of certain amino acids and amino acid residues were determined by their orientation on the surface and depended on the electrode potential (E).Abbreviations SERS
surface enhanced Raman scattering
- Trp
tryptophan
- Tyr
tyrosine
- Phe
phenylalanine
- E
electrode potential
- ORC
oxidation-reduction cycle 相似文献
3.
Histochemical studies on localisation of lipids, lipase and phosphomonoeste-rases in the crown skin of male baya during non-breeding
and breeding seasons were carried out. The results indicated a high turnover rate for lipid synthesis and its utilisation,
increased acid and alkaline phosphatase activities in the crown skin of breeding male baya as compared to that in non-breeding
season. It is surmised that crown skin of male baya becomes metabolically hyperactive during breeding season aiding processes
such as cell growth and proliferation, keratinization and production of coloured feathers related to breeding. 相似文献
4.
5.
Application of mefluidide (N-[2,4-dimethyl-5-([(trifluoromethyl)sulfonyl]amino) phenyl]acetamide) inhibits plant development in perennial grasses. This study examined the effect of mefluidide on the morphological development and digestibility of sorghum. In the greenhouse, 5.9 × 10–5 g active ingredient (a.i.) plant–1 applied at the seedling, eight-leaf and boot stages reduced mean plant height 70%, 59%, and 2%, respectively. Heights were also reduced 14%, 15% and 35% by 5.9 × 10–8, 5.9 × 10–7 and 5.9 × 10–6 gram a.i. plant–1 applied at the eight-leaf stage. Field application of 0.26 or 0.52 kg ha–1 mefluidide at either the eight-leaf or flagleaf stage reduced mean plant height of all cultivars. Basal tiller numbers increased 319% 28 d, and dry matter production was reduced 65% 42 d following mefluidide application at the eight-leaf stage. Treated stems were 34% higher and treated leaves were 7% higher in cellulase dry matter digestibility than control plants following mefluidide application at the eight-leaf stage. These results indicate that mefluidide application to vegetative stages in sorghum may enhance the forage value of the plants while it inhibits normal plant growth. 相似文献
6.
Lignocellulose biotransformation with immobilized cellulase,d-glucose oxidase and fungal peroxidases
Three enzymes, cellulase [see 1,4-(1,3;1,4)-β-d-glucan 4-glucanohydrolase, EC 3.2.1.4], d-glucose oxidase (β-d-glucose: oxygen 1-oxidoreductase, EC 1.1.3.4) and peroxidase (donor:hydrogen peroxide oxidoreductase, EC 1.11.1.7) immobilized on glass beads, have been incubated with lignocellulose. Fungal peroxidases from Trametes versicolor and Inonotus radiatus when mixed with cellulase and d-glucose oxidase were able to liberate phenolic compounds and d-glucose from lignocellulose. Three lignin monomers were identified. When the immobilized enzymes were incubated individually with lignocellulose they did not degrade lignin. 相似文献
7.
A. Shteyer D. Gazit I. Binderman I. A. Bab 《In vitro cellular & developmental biology. Plant》1987,23(1):15-20
Summary Cells of the dental papilla are capable of odontoblastic, fibroblastic, and endothelial differentiation and formation of dentin
and the dental pulp. In the present study dental papilla cells, obtained from human tooth buds (HDP cells), were cultured
in vitro through 3 to 7 passages. After exposure to prostaglandin E2 there was a marked decrease in intracellular cyclic AMP (cAMP) levels as compared to hormone-free controls. Parathyroid hormone
and calcitonin had stimulatory effects with 1 and 2 log increases in cAMP, respectively. The HDP cells showed moderate activity
of alkaline phosphatase, 1 log higher than that of hamster kidney fibroblasts (BHK 13) and 1 log lower than that of osteoblastic
osteosarcoma cells (ROS 17/2). When cultured for 4 or 8 wk in diffusion chambers (DC) implanted in athymic mice, many of the
HDP cells underwent odontoblastic morphodifferentiation with very long, single processes extending into the matrix. This matrix
contained banded and unbanded collagen fibers. Neither light nor electron microscopy of the DC content revealed mineral deposits.
These results suggest that HDP cells have an intrinsic potential for partial odontoblastic differentiation; inductive signals
like those originating from odontogenic epithelium are probably essential for the completion of hard tissue formation. 相似文献
8.
Efficient preparation of spheroplasts fromCandida utilis, Saccharomyces cerevisiae, andSchizosaccharomyces pombe, using a purified mixture of enzymes fromTrichoderma harzianum, is described. Limitations of other methods, and differences between yeasts are demonstrated. 相似文献
9.
Psychoactive drugs like chlorpromazine (CPZ), imipramine, lithium and amphetamine in one way or another affect behaviour. The drug responses are presumably mediated by inducing a change in the activity of membrane bound enzymes. CPZ is very potent in inhibiting the alkaline phosphatase activity in rat brain. The combined effect of CPZ with other drugs shows that CPZ and imipramine together inhibit the enzyme activity significantly greater than the individual inhibition either by CPZ or by imipramine alone. Effective inhibition of the alkaline phosphatase activity with a single drug or combined drugs may lead to a change in neuronal permeability through glucocorticoids thereby affecting mood. 相似文献
10.
A thermophilic Bacillus stearothermophilus strain AP-4 excreting a thermostable alkaline protease, was isolated from a local compost. Maximum activity of protease (250 U/ml) was after 36 h growth in broth at pH 9.0 and at 55°C. The protease was optimally active at pH 9.0 and 55°C and was stable in 5 mm CaCl2. The enzyme was completely inactivated by PMSF, EDTA and -mercaptoethanol. It is therefore a metal ion-dependent, alkaline, serine protease.R. Dhandapani and R. Vijayaragavan are with the Centre for Plant Molecular Biology & Biotechnology, Tamil Nadu Agricultural University, Coimbatore 641 003, India 相似文献