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1.
Probing the interactions between carboxylated multi‐walled carbon nanotubes and copper–zinc superoxide dismutase at a molecular level
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In order to evaluate the toxicity of multi‐walled carbon nanotubes (MWCNTs‐COOH) at a molecular level, the effect of MWCNTs‐COOH on antioxidant enzyme copper–zinc superoxide dismutase (Cu/ZnSOD) was investigated using fluorescence spectroscopy, UV/vis absorption spectroscopy, circular dichroism (CD) spectroscopy and isothermal titration calorimetry (ITC). By deducting the inner filter effect (IFE), the fluorescence emission spectra and synchronous fluorescence spectra indicated that there were interactions between MWCNTs‐COOH and Cu/ZnSOD. Moreover, the microenvironment of the amino acid residues in the enzyme was changed slightly. The UV/vis absorption and CD spectroscopic results showed appreciable conformational changes in Cu/ZnSOD. However, the results of a Cu/ZnSOD activity determination did not show any significant difference. In other words, MWCNTs‐COOH has no significant effect on enzyme activity. The ITC results showed that the binding of MWCNTs‐COOH to Cu/ZnSOD was a weak endothermic process, indicating that the predominant force of the binding was hydrophobic interaction. Moreover, it was essential to consider the IFE in fluorescence assays, which might affect the accuracy and precision of the results. The above results are helpful in evaluating the oxidative stress induced by MWCNTs‐COOH in vivo. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
2.
Shahin Negari Jeff Sulpher Francesca Pacello Keely Ingrey Andrea Battistoni B. Craig Lee 《Biometals》2008,21(3):249-258
The Cu,Zn superoxide dismutase (Cu,ZnSOD) from Haemophilus
ducreyi is the only enzyme of this class which binds a heme molecule at its dimer interface. To explore the role of the enzyme in
this heme-obligate bacterium, a sodC mutant was created by insertional inactivation. No difference in growth rate was observed during heme limitation. In contrast,
under heme rich conditions growth of the sodC mutant was impaired compared to the wild type strain. This growth defect was abolished by supplementation of exogenous catalase.
Genetic complementation of the sodC mutant in trans demonstrated that the enzymatic property or the heme-binding activity of the protein could repair the growth
defect of the sodC mutant. These results indicate that Cu,ZnSOD protects Haemophilus ducreyi from heme toxicity. 相似文献
3.
Paksi Z Jancsó A Pacello F Nagy N Battistoni A Gajda T 《Journal of inorganic biochemistry》2008,102(9):1700-1710
The Cu,Zn superoxide dismutase (Cu,ZnSOD) isolated from Haemophilus ducreyi possesses a His-rich N-terminal metal binding domain, which has been previously proposed to play a copper(II) chaperoning role. To analyze the metal binding ability and selectivity of the histidine-rich domain we have carried out thermodynamic and solution structural analysis of the copper(II) and zinc(II) complexes of a peptide corresponding to the first 11 amino acids of the enzyme (H2N-HGDHMHNHDTK-OH, L). This peptide has highly versatile metal binding ability and provides one and three high affinity binding sites for zinc(II) and copper(II), respectively. In equimolar solutions the MHL complexes are dominant in the neutral pH-range with protonated lysine ε-amino group. As a consequence of its multidentate nature, L binds zinc and copper with extraordinary high affinity (KD,Zn = 1.6 × 10−9 M and KD,Cu = 5.0 × 10−12 M at pH 7.4) and appears as the strongest zinc(II) and copper(II) chelator between the His-rich peptides so far investigated. These KD values support the already proposed role of the N-terminal His-rich region of H. ducreyi Cu,ZnSOD in copper recruitment under metal starvation, and indicate a similar function in the zinc(II) uptake, too. The kinetics of copper(II) transfer from L to the active site of Cu-free N-deleted H. ducreyi Cu,ZnSOD showed significant pH and copper-to-peptide ratio dependence, indicating specific structural requirements during the metal ion transfer to the active site. Interestingly, the complex CuHL has significant superoxide dismutase like activity, which may suggest multifunctional role of the copper(II)-bound N-terminal His-rich domain of H. ducreyi Cu,ZnSOD. 相似文献
4.
Imre Tör? Francesca Pacello Andrea Battistoni Kristina Djinovi?-Carugo 《Journal of molecular biology》2009,386(2):406-87
The Cu,Zn superoxide dismutase from Haemophilus ducreyi is characterized by the unique ability to bind heme at its dimer interface. Here we report the high-resolution crystal structures of this protein in the heme-loaded (holo) and heme-free (apo) forms. Heme is asymmetrically bound between the two enzyme subunits, where heme iron is coordinated by two histidine residues, His64 and His 124, provided by the two subunits. Moreover, the binding of heme to the protein is ensured by stabilizing contacts between the prosthetic group and a limited number of other residues, most of which are not present in other bacterial enzyme variants. We show that the introduction of only three mutations at the dimer interface of the enzyme from Haemophilus parainfluenzae, a closely related bacterial species, is sufficient to induce heme-binding ability by this enzyme variant. Heme binding does not alter protein activity. Moreover, the binding of the prosthetic group does not induce any significant structural perturbation at the subunit level and requires only limited local structural rearrangements that widen the cleft at the dimer interface and cause a limited shift in the relative orientation between the subunits. The presence of a preformed heme-binding pocket and the significant solvent exposure of the cofactor to the solvent are compatible with the suggested protective role of the enzyme against heme toxicity or with its involvement in heme trafficking in the periplasmic space. 相似文献
5.
东方山羊豆Cu/ZnSOD基因的克隆及表达分析 总被引:2,自引:0,他引:2
超氧化物歧化酶是一种广泛存在于真核生物中的金属酶类,在植物的抗逆性中起到重要的作用。文章采用RACE方法,从东方山羊豆中克隆了Cu/ZnSOD基因,并对其进行了初步分析。该基因cDNA序列全长935 bp,开放阅读框600 bp,编码199个氨基酸,蛋白质分子量为20.35 kDa。通过实时荧光定量PCR结果分析,该基因在东方山羊豆叶中表达量最多,茎中次之,根中最少。在NaCl和PEG诱导下,Cu/ZnSOD基因表达量先上调后下降。NaCl诱导24 h后,该基因的表达量显著低于对照。ABA胁迫抑制了该基因的表达。亚细胞定位结果表明,Cu/ZnSOD蛋白定位于叶绿体中。实验结果证明,Cu/ZnSOD基因主要在东方山羊豆的绿色组织中表达,在抵抗渗透性胁迫方面起到一定作用。 相似文献
6.
The interaction of Cu,ZnSOD with H2O2 generates an oxidant at the active site that can then cause either the inactivation of this enzyme or the oxidation of a variety of exogenous substrates. We show that the rate of inactivation, imposed by 10-mM H2O2 at 25 degrees C and pH 7.2, is not influenced by 10-mM HCO3-; whereas the oxidation of 2,2'-azino-bis-[3-ethylbenzothiazoline sulfonate] (ABTS=) is virtually completely dependent upon HCO3-. The reduction of the active site Cu(II) by H2O2, which precedes inactivation of the enzyme, occurred at the same rate in phosphate buffer with or without bicarbonate added. These results indicate that HCO3- does not play a role in facilitating the interaction of H2O2 with the active site copper, but they can be accommodated by the proposal that HCO3- is oxidized to HCO3*, which then diffuses from that site and causes the oxidation of substrates, such as ABTS=, that are too large to traverse the solvent access channel to the Cu(II). 相似文献
7.
Wiswedel I Keilhoff G Dörner L Navarro A Böckelmann R Bonnekoh B Gardemann A Gollnick H 《Free radical research》2007,41(9):1017-1027
UVB irradiation of human skin is known to induce pathophysiological processes as oxidative stress and inflammation. HaCaT keratinocytes represent a well-established in vitro model system to investigate the influence of UVB irradiation on cell cultures. It was the aim of these investigations to study the effects of moderate UVB doses on cellular and mitochondrial integrity of HaCaT keratinocytes, biomarkers of oxidative stress and antioxidant protection by superoxide dismutases. F2-isoprostane concentrations were UVB dose-dependently enhanced reaching a plateau at 50 mJ/cm2. Cell viability was reduced and apoptosis was enhanced with increasing UVB doses. The activities of the respiratory chain complexes were practically not altered at lower UVB doses, up to 50 mJ/cm2, whereas remarkable decreases, also for the levels of cardiolipin species, were seen at 100 mJ/cm2. As an adaptive response to the enhanced oxidative stress, protein levels of MnSOD increased about 3-fold at 50 mJ/cm2 and decreased at higher doses. From the data it can be concluded that keratinocytes are sufficiently protected at low UVB doses, whereas higher doses lead to irreversible cell damage. 相似文献
8.
Abdel-Ghany SE Burkhead JL Gogolin KA Andrés-Colás N Bodecker JR Puig S Peñarrubia L Pilon M 《FEBS letters》2005,579(11):2307-2312
In plant chloroplasts two superoxide dismutase (SOD) activities occur, FeSOD and Cu/ZnSOD, with reciprocal regulation in response to copper availability. This system presents a unique model to study the regulation of metal-cofactor delivery to an organelle. The Arabidopsis thaliana gene AtCCS encodes a functional homolog to yeast Ccs1p/Lys7p, a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD. AtCCS mRNA expression levels are upregulated in response to Cu-feeding and senescence. We propose that AtCCS expression is regulated to allow the most optimal use of Cu for photosynthesis. 相似文献
9.
Zwirska-Korczala K Adamczyk-Sowa M Polaniak R Sowa P Birkner E Drzazga Z Brzozowski T Konturek SJ 《Biological trace element research》2004,102(1-3):227-243
Effects of melatonin, extremely-low-frequency magnetic field (ELF-MF), and their combination on AT478 murine squamous cell
carcinoma line were studied. Manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (Cu/ZnSOD), and glutathione
peroxidase (GSH-Px) were used as markers of cells antioxidative status, and malondialdehyde (MDA) level was used as a marker
of lipid peroxidation. After melatonin treatment, antioxidative enzyme activities were increased and MDA level was decreased.
Application of ELF-MF on treated cells caused an increase of both superoxide dismutases activity and MDA level, but influence
of ELF-MF on GSH-Px activity was negligible. All enzyme activity in culture medium containing melatonin (10−3, 10−4, 10−5
M) after exposure to ELF-MF were significantly diminished compared to cells treated only with melatonin. Also MDA levels after
combined treatment with melatonin and ELF-MF were significantly decreased. Observed changes were statistically significant
(p<0.05). These results strongly suggest that ELF-MF attenuates antioxidative actions of melatonin on cellular level. 相似文献
10.
《Free radical research》2013,47(5):299-309
Copper, zinc superoxide dismutase (Cu, ZnSOD) and manganese superoxide dismutase (MnSOD) activities were measured in mouse large intestinal mucosa during dimethylhydrazine (DMH) carcinogenesis. Mice were divided into five groups. Group A was subcutaneously injected with DMH (20mg/kg) weekly and fed with a diet containing 0.2% cholic acid (C) and 0.8% indole (I). Group B was injected with DMH and given indole feeding. Group C was treated with DMH injection and cholic acid feeding. Group D was given DMH injection alone. Group E was an age-matched control group given 0.9% NaCl injection. The experiment last 21 weeks. The Cu, ZnSOD activity of intestinal mucosa in group A animals began to increase significantly at the 7th week of the experiment. In groups B, C and D, however, this enzyme was not elevated statistically until the 16th week, and then each of these groups kept an increased Cu, ZnSOD level the rest of the experimental period. MnSOD activity was elevated statistically in group C animals at the 7th week. The enzyme activity in group A and D animals increased at the 9th week, but the enzyme activity did not increase statistically until the 11th week in group B. After the 16th week of the experiment the increased activity of MnSOD in all experimental groups returned to the level of the control group. Large intestinal cancer tissues had increased Cu, ZnSOD activity and decreased MnSOD activity. 相似文献